################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbrPI BcgI BciVI BfrI BlnI BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmBI Bsp1407I BspEI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1039 GTCTAC GT^[AC][GT]AC AcyI 1321 GGCGCC G[GA]^CG[CT]C AocI 1896 CCTCAGG CC^T[ACGT]AGG BalI 136 TGGCCA TGG^CCA BamHI 641 GGATCC G^GATCC BbeI 1321 GGCGCC GGCGC^C BbsI 837 GAAGAC GAAGAC(2/6) BclI 350 TGATCA T^GATCA BfaI 1942 CTAG C^TAG BglII 1733 AGATCT A^GATCT BlpI 33 GCTCAGC GC^T[ACGT]AGC Bpu1102I 33 GCTCAGC GC^T[ACGT]AGC BpuAI 837 GAAGAC GAAGAC(2/6) BsaHI 1321 GGCGCC G[GA]^CG[CT]C BsgI 360 GTGCAG GTGCAG(16/14) BsmI 773 GAATGC GAATGC(1/-1) BspHI 1358 TCATGA T^CATGA BsrFI 1115 ACCGGC [GA]^CCGG[CT] BssSI 936 CACGAG CACGAG(-5/-1) BstAPI 1770 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1859 GGTGACC G^GT[ACGT]ACC BstPI 1859 GGTGACC G^GT[ACGT]ACC Bsu36I 1896 CCTCAGG CC^T[ACGT]AGG CelII 33 GCTCAGC GC^T[ACGT]AGC Cfr10I 1115 ACCGGC [GA]^CCGG[CT] DraIII 354 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1896 CCTCAGG CC^T[ACGT]AGG EcoNI 1413 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1859 GGTGACC G^GT[ACGT]ACC FbaI 350 TGATCA T^GATCA Hin1I 1321 GGCGCC G[GA]^CG[CT]C KasI 1321 GGCGCC G^GCGCC MaeI 1942 CTAG C^TAG MaeII 326 ACGT A^CGT MluNI 136 TGGCCA TGG^CCA MscI 136 TGGCCA TGG^CCA MseI 1307 TTAA T^TAA NarI 1321 GGCGCC GG^CGCC NspI 668 GCATGT [GA]CATG^[CT] PflMI 1407 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1358 TCATGA T^CATGA SfoI 1321 GGCGCC GGC^GCC SmaI 620 CCCGGG CCC^GGG Tru9I 1307 TTAA T^TAA Tsp509I 436 AATT ^AATT Van91I 1407 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 620 CCCGGG C^CCGGG XmaI 620 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BsaWI 769 ACCGGA [AT]^CCGG[AT] 1343 ACCGGA BsmAI 1347 GAGAC GTCTC(1/5) 1938 GTCTC BspDI 409 ATCGAT AT^CGAT 853 ATCGAT BsrBI 973 CCGCTC CCGCTC(-3/-3) 913 GAGCGG ClaI 409 ATCGAT AT^CGAT 853 ATCGAT EaeI 136 TGGCCA [CT]^GGCC[GA] 244 TGGCCG HgaI 313 GCGTC GACGC(5/10) 1074 GCGTC MslI 252 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 768 CACCGGAATG XcmI 281 CCACCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1541 CCAACGAGTGCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII