##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BbrPI BcgI BciVI
BfrI BlnI BsaAI BsaBI BsaI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspEI BspLU11I BsrDI BsrGI BssHII
Bst1107I BstBI BstZ17I CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 1039 GTCTAC GT^[AC][GT]AC
AcyI 1321 GGCGCC G[GA]^CG[CT]C
AocI 1896 CCTCAGG CC^T[ACGT]AGG
BalI 136 TGGCCA TGG^CCA
BamHI 641 GGATCC G^GATCC
BbeI 1321 GGCGCC GGCGC^C
BbsI 837 GAAGAC GAAGAC(2/6)
BclI 350 TGATCA T^GATCA
BfaI 1942 CTAG C^TAG
BglII 1733 AGATCT A^GATCT
BlpI 33 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 33 GCTCAGC GC^T[ACGT]AGC
BpuAI 837 GAAGAC GAAGAC(2/6)
BsaHI 1321 GGCGCC G[GA]^CG[CT]C
BsgI 360 GTGCAG GTGCAG(16/14)
BsmI 773 GAATGC GAATGC(1/-1)
BspHI 1358 TCATGA T^CATGA
BsrFI 1115 ACCGGC [GA]^CCGG[CT]
BssSI 936 CACGAG CACGAG(-5/-1)
BstAPI 1770 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1859 GGTGACC G^GT[ACGT]ACC
BstPI 1859 GGTGACC G^GT[ACGT]ACC
Bsu36I 1896 CCTCAGG CC^T[ACGT]AGG
CelII 33 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1115 ACCGGC [GA]^CCGG[CT]
DraIII 354 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1896 CCTCAGG CC^T[ACGT]AGG
EcoNI 1413 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1859 GGTGACC G^GT[ACGT]ACC
FbaI 350 TGATCA T^GATCA
Hin1I 1321 GGCGCC G[GA]^CG[CT]C
KasI 1321 GGCGCC G^GCGCC
MaeI 1942 CTAG C^TAG
MaeII 326 ACGT A^CGT
MluNI 136 TGGCCA TGG^CCA
MscI 136 TGGCCA TGG^CCA
MseI 1307 TTAA T^TAA
NarI 1321 GGCGCC GG^CGCC
NspI 668 GCATGT [GA]CATG^[CT]
PflMI 1407 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1358 TCATGA T^CATGA
SfoI 1321 GGCGCC GGC^GCC
SmaI 620 CCCGGG CCC^GGG
Tru9I 1307 TTAA T^TAA
Tsp509I 436 AATT ^AATT
Van91I 1407 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 620 CCCGGG C^CCGGG
XmaI 620 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 9 enzymes) #
###################################
BsaWI 769 ACCGGA [AT]^CCGG[AT]
1343 ACCGGA
BsmAI 1347 GAGAC GTCTC(1/5)
1938 GTCTC
BspDI 409 ATCGAT AT^CGAT
853 ATCGAT
BsrBI 973 CCGCTC CCGCTC(-3/-3)
913 GAGCGG
ClaI 409 ATCGAT AT^CGAT
853 ATCGAT
EaeI 136 TGGCCA [CT]^GGCC[GA]
244 TGGCCG
HgaI 313 GCGTC GACGC(5/10)
1074 GCGTC
MslI 252 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
768 CACCGGAATG
XcmI 281 CCACCATGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1541 CCAACGAGTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII