##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AocI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BbvCI BciVI BfaI BfrI BlnI
BsaAI BsaBI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoRV EcoT22I FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 1737 CGCG CG^CG
AccIII 1442 TCCGGA T^CCGGA
AcsI 485 GAATTC [GA]^AATT[CT]
AflIII 818 ACGTGT A^C[GA][CT]GT
Alw44I 652 GTGCAC G^TGCAC
AlwNI 604 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 779 GGGCCC GGGCC^C
ApaLI 652 GTGCAC G^TGCAC
ApoI 485 GAATTC [GA]^AATT[CT]
Asp700I 444 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 677 CGACAGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1663 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1216 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1216 GCTAAGC GC^T[ACGT]AGC
BsaWI 1442 TCCGGA [AT]^CCGG[AT]
BseAI 1442 TCCGGA T^CCGGA
BsiEI 13 CGGCCG CG[GA][CT]^CG
BspEI 1442 TCCGGA T^CCGGA
BsrBI 439 GAGCGG CCGCTC(-3/-3)
BstEII 213 GGTGACC G^GT[ACGT]ACC
BstPI 213 GGTGACC G^GT[ACGT]ACC
BstUI 1737 CGCG CG^CG
BstXI 1719 CCACATTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1216 GCTAAGC GC^T[ACGT]AGC
EagI 13 CGGCCG C^GGCCG
EarI 829 GAAGAG CTCTTC(1/4)
EclXI 13 CGGCCG C^GGCCG
Eco52I 13 CGGCCG C^GGCCG
EcoO65I 213 GGTGACC G^GT[ACGT]ACC
EcoRI 485 GAATTC G^AATTC
Ksp632I 829 GAAGAG CTCTTC(1/4)
MroI 1442 TCCGGA T^CCGGA
MvnI 1737 CGCG CG^CG
NspI 1608 GCATGT [GA]CATG^[CT]
PleI 1446 GACTC GAGTC(4/5)
SacI 1647 GAGCTC GAGCT^C
SapI 829 GAAGAGC GCTCTTC(1/4)
StuI 186 AGGCCT AGG^CCT
TaqI 418 TCGA T^CGA
TthHB8I 418 TCGA T^CGA
XmnI 444 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 766 GGCGCC G[GA]^CG[CT]C
1324 GGCGCC
AfaI 1369 GTAC GT^AC
1779 GTAC
AlwI 288 GGATC GGATC(4/5)
752 GGATC
BalI 389 TGGCCA TGG^CCA
792 TGGCCA
BbeI 766 GGCGCC GGCGC^C
1324 GGCGCC
BbsI 546 GAAGAC GAAGAC(2/6)
1393 GTCTTC
BclI 377 TGATCA T^GATCA
1698 TGATCA
BglII 101 AGATCT A^GATCT
623 AGATCT
BpuAI 546 GAAGAC GAAGAC(2/6)
1393 GTCTTC
BsaHI 766 GGCGCC G[GA]^CG[CT]C
1324 GGCGCC
BsaI 1549 GAGACC GGTCTC(1/5)
582 GGTCTC
BsmAI 1549 GAGAC GTCTC(1/5)
583 GTCTC
BspMI 1424 ACCTGC ACCTGC(4/8)
123 GCAGGT
BsrDI 37 GCAATG GCAATG(2/0)
1465 GCAATG
CfoI 767 GCGC GCG^C
1325 GCGC
EcoNI 1173 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1491 CCTGGCAGAGG
FauI 1153 CCCGC CCCGC(4/6)
1738 GCGGG
FbaI 377 TGATCA T^GATCA
1698 TGATCA
HaeII 766 GGCGCC [GA]GCGC^[CT]
1324 GGCGCC
HhaI 767 GCGC GCG^C
1325 GCGC
Hin1I 766 GGCGCC G[GA]^CG[CT]C
1324 GGCGCC
HinP1I 767 GCGC G^CGC
1325 GCGC
KasI 766 GGCGCC G^GCGCC
1324 GGCGCC
MaeII 818 ACGT A^CGT
1559 ACGT
MluNI 389 TGGCCA TGG^CCA
792 TGGCCA
MscI 389 TGGCCA TGG^CCA
792 TGGCCA
NarI 766 GGCGCC GG^CGCC
1324 GGCGCC
PpuMI 1081 GGGTCCC [GA]G^G[AT]CC[CT]
1421 GGGACCT
RsaI 1369 GTAC GT^AC
1779 GTAC
SfoI 766 GGCGCC GGC^GCC
1324 GGCGCC
TfiI 18 GATTC G^A[AT]TC
399 GATTC
Tsp509I 486 AATT ^AATT
933 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AluI AspHI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI Tsp45I TspRI Van91I XhoII