################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI Asp700I Asp718I AspEI AviII BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BsaAI BsaHI BseAI BsgI BsiWI BsmBI BspEI BsrBI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI CpoI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PinAI PmaCI PmeI PmlI PshAI PstI PvuI RsrII SacII SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 19 GTCGAC GT^[AC][GT]AC AclI 1720 AACGTT AA^CGTT AflIII 1083 ACATGT A^C[GA][CT]GT Alw44I 888 GTGCAC G^TGCAC ApaLI 888 GTGCAC G^TGCAC AvaI 1073 CTCGAG C^[CT]CG[GA]G AvrII 1240 CCTAGG C^CTAGG BalI 846 TGGCCA TGG^CCA BamHI 238 GGATCC G^GATCC BbsI 964 GTCTTC GAAGAC(2/6) BlnI 1240 CCTAGG C^CTAGG BlpI 1425 GCTCAGC GC^T[ACGT]AGC BpmI 2067 CTCCAG CTGGAG(16/14) Bpu1102I 1425 GCTCAGC GC^T[ACGT]AGC BpuAI 964 GTCTTC GAAGAC(2/6) BsaBI 197 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 755 GAGGAG GAGGAG(10/8) BsmI 1792 GAATGC GAATGC(1/-1) BsoBI 1073 CTCGAG C^[CT]CG[GA]G Bsp1407I 2560 TGTACA T^GTACA BspDI 1142 ATCGAT AT^CGAT BspLU11I 1083 ACATGT A^CATGT BsrGI 2560 TGTACA T^GTACA BssSI 330 CACGAG CACGAG(-5/-1) BstAPI 251 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2035 CCAGAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1425 GCTCAGC GC^T[ACGT]AGC ClaI 1142 ATCGAT AT^CGAT DraIII 330 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 22 GACGACTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1536 GAATTC G^AATTC EcoRV 2278 GATATC GAT^ATC HphI 1781 GGTGA GGTGA(8/7) MamI 197 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 846 TGGCCA TGG^CCA MscI 846 TGGCCA TGG^CCA MslI 2358 CATTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PaeR7I 1073 CTCGAG C^TCGAG PflMI 1140 CCATCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2090 AGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 1720 AACGTT AA^CGTT SalI 19 GTCGAC G^TCGAC ScaI 87 AGTACT AGT^ACT SspBI 2560 TGTACA T^GTACA Van91I 1140 CCATCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 1073 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 2070 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG 2620 CAGCTGCTG AspI 25 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 70 GACCCCGTC BclI 645 TGATCA T^GATCA 838 TGATCA BcnI 1893 CCCGG CC^[GC]GG 2094 CCCGG BglI 1179 GCCAGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2152 GCCCTGGGGGC BglII 857 AGATCT A^GATCT 2057 AGATCT BsaWI 77 TCCGGT [AT]^CCGG[AT] 993 TCCGGT BsiEI 995 CGGTCG CG[GA][CT]^CG 1210 CGACCG BspMI 466 GCAGGT ACCTGC(4/8) 1881 GCAGGT BsrFI 869 GCCGGT [GA]^CCGG[CT] 1956 GCCGGT Cac8I 462 GCTGGC GC[ACGT]^[ACGT]GC 724 GCCAGC Cfr10I 869 GCCGGT [GA]^CCGG[CT] 1956 GCCGGT DraII 2090 AGGTCCC [GA]G^G[ACGT]CC[CT] 2158 GGGGCCT EaeI 144 CGGCCA [CT]^GGCC[GA] 846 TGGCCA EcoO109I 2090 AGGTCCC [GA]G^G[ACGT]CC[CT] 2158 GGGGCCT FbaI 645 TGATCA T^GATCA 838 TGATCA NciI 1893 CCCGG CC^[GC]GG 2094 CCCGG NdeI 1189 CATATG CA^TATG 1283 CATATG NspI 313 ACATGC [GA]CATG^[CT] 1083 ACATGT PacI 601 TTAATTAA TTAAT^TAA 2508 TTAATTAA PflFI 25 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 70 GACCCCGTC PleI 25 GACTC GAGTC(4/5) 456 GAGTC SapI 1174 GAAGAGC GCTCTTC(1/4) 2029 GAAGAGC SfcI 289 CTGTAG C^T[GA][CT]AG 832 CTACAG SmlI 451 CTCAAG C^T[CT][GA]AG 1073 CTCGAG TfiI 168 GATTC G^A[AT]TC 1037 GAATC TspRI 835 CAGTG CA[GC]TG[ACGT][ACGT]^ 2125 CAGTG Tth111I 25 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC 70 GACCCCGTC XbaI 854 TCTAGA T^CTAGA 874 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AseI AsnI AspHI AvaII BanII BbvI BfaI BmyI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PshBI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SspI StyI TaqI Tru9I TseI Tsp45I Tsp509I TthHB8I XhoII