##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI AocI Aor51HI
ApaI AscI Asp700I Asp718I AspEI
AviII BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BsaAI BsaHI
BseAI BsgI BsiWI BsmBI BspEI
BsrBI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CfoI CpoI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PinAI
PmaCI PmeI PmlI PshAI PstI
PvuI RsrII SacII SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI XcmI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 19 GTCGAC GT^[AC][GT]AC
AclI 1720 AACGTT AA^CGTT
AflIII 1083 ACATGT A^C[GA][CT]GT
Alw44I 888 GTGCAC G^TGCAC
ApaLI 888 GTGCAC G^TGCAC
AvaI 1073 CTCGAG C^[CT]CG[GA]G
AvrII 1240 CCTAGG C^CTAGG
BalI 846 TGGCCA TGG^CCA
BamHI 238 GGATCC G^GATCC
BbsI 964 GTCTTC GAAGAC(2/6)
BlnI 1240 CCTAGG C^CTAGG
BlpI 1425 GCTCAGC GC^T[ACGT]AGC
BpmI 2067 CTCCAG CTGGAG(16/14)
Bpu1102I 1425 GCTCAGC GC^T[ACGT]AGC
BpuAI 964 GTCTTC GAAGAC(2/6)
BsaBI 197 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 755 GAGGAG GAGGAG(10/8)
BsmI 1792 GAATGC GAATGC(1/-1)
BsoBI 1073 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2560 TGTACA T^GTACA
BspDI 1142 ATCGAT AT^CGAT
BspLU11I 1083 ACATGT A^CATGT
BsrGI 2560 TGTACA T^GTACA
BssSI 330 CACGAG CACGAG(-5/-1)
BstAPI 251 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2035 CCAGAAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1425 GCTCAGC GC^T[ACGT]AGC
ClaI 1142 ATCGAT AT^CGAT
DraIII 330 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 22 GACGACTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1536 GAATTC G^AATTC
EcoRV 2278 GATATC GAT^ATC
HphI 1781 GGTGA GGTGA(8/7)
MamI 197 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 846 TGGCCA TGG^CCA
MscI 846 TGGCCA TGG^CCA
MslI 2358 CATTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 1073 CTCGAG C^TCGAG
PflMI 1140 CCATCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2090 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1720 AACGTT AA^CGTT
SalI 19 GTCGAC G^TCGAC
ScaI 87 AGTACT AGT^ACT
SspBI 2560 TGTACA T^GTACA
Van91I 1140 CCATCGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1073 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 2070 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
2620 CAGCTGCTG
AspI 25 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
70 GACCCCGTC
BclI 645 TGATCA T^GATCA
838 TGATCA
BcnI 1893 CCCGG CC^[GC]GG
2094 CCCGG
BglI 1179 GCCAGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2152 GCCCTGGGGGC
BglII 857 AGATCT A^GATCT
2057 AGATCT
BsaWI 77 TCCGGT [AT]^CCGG[AT]
993 TCCGGT
BsiEI 995 CGGTCG CG[GA][CT]^CG
1210 CGACCG
BspMI 466 GCAGGT ACCTGC(4/8)
1881 GCAGGT
BsrFI 869 GCCGGT [GA]^CCGG[CT]
1956 GCCGGT
Cac8I 462 GCTGGC GC[ACGT]^[ACGT]GC
724 GCCAGC
Cfr10I 869 GCCGGT [GA]^CCGG[CT]
1956 GCCGGT
DraII 2090 AGGTCCC [GA]G^G[ACGT]CC[CT]
2158 GGGGCCT
EaeI 144 CGGCCA [CT]^GGCC[GA]
846 TGGCCA
EcoO109I 2090 AGGTCCC [GA]G^G[ACGT]CC[CT]
2158 GGGGCCT
FbaI 645 TGATCA T^GATCA
838 TGATCA
NciI 1893 CCCGG CC^[GC]GG
2094 CCCGG
NdeI 1189 CATATG CA^TATG
1283 CATATG
NspI 313 ACATGC [GA]CATG^[CT]
1083 ACATGT
PacI 601 TTAATTAA TTAAT^TAA
2508 TTAATTAA
PflFI 25 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
70 GACCCCGTC
PleI 25 GACTC GAGTC(4/5)
456 GAGTC
SapI 1174 GAAGAGC GCTCTTC(1/4)
2029 GAAGAGC
SfcI 289 CTGTAG C^T[GA][CT]AG
832 CTACAG
SmlI 451 CTCAAG C^T[CT][GA]AG
1073 CTCGAG
TfiI 168 GATTC G^A[AT]TC
1037 GAATC
TspRI 835 CAGTG CA[GC]TG[ACGT][ACGT]^
2125 CAGTG
Tth111I 25 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
70 GACCCCGTC
XbaI 854 TCTAGA T^CTAGA
874 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaII
BanII BbvI BfaI BmyI BsaI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI BstYI Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PshBI PvuII RcaI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SspI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TthHB8I XhoII