##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AlwI
AocI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbvCI
BciVI BclI BfrI BglII BlnI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
ClaI CpoI DraIII DrdI Eam1105I
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI ScaI SexAI SfiI SfuI
SnaBI SpeI SphI SspBI SspI
StuI SwaI Tsp509I Tth111I Van91I
XbaI XcmI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 1363 GTAC GT^AC
AlwNI 523 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
BbsI 1093 GTCTTC GAAGAC(2/6)
BcgI 120 CGAGGAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 538 CTAG C^TAG
BlpI 1119 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1119 GCTCAGC GC^T[ACGT]AGC
BpuAI 1093 GTCTTC GAAGAC(2/6)
BspLU11I 815 ACATGT A^CATGT
BstXI 778 CCACGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1119 GCTCAGC GC^T[ACGT]AGC
DraI 1261 TTTAAA TTT^AAA
EagI 260 CGGCCG C^GGCCG
EclXI 260 CGGCCG C^GGCCG
Eco52I 260 CGGCCG C^GGCCG
KspI 263 CCGCGG CCGC^GG
MaeI 538 CTAG C^TAG
MseI 1262 TTAA T^TAA
MslI 378 CATGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 815 ACATGT [GA]CATG^[CT]
PvuII 1101 CAGCTG CAG^CTG
RsaI 1363 GTAC GT^AC
SacII 263 CCGCGG CCGC^GG
SapI 987 GCTCTTC GCTCTTC(1/4)
SbfI 1068 CCTGCAGG CCTGCA^GG
Sse8387I 1068 CCTGCAGG CCTGCA^GG
TfiI 808 GAATC G^A[AT]TC
Tru9I 1262 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 401 ACACGT A^C[GA][CT]GT
815 ACATGT
BseRI 121 GAGGAG GAGGAG(10/8)
505 GAGGAG
BsgI 682 CTGCAC GTGCAG(16/14)
1018 CTGCAC
BsiEI 260 CGGCCG CG[GA][CT]^CG
1077 CGACCG
BsrBI 985 CCGCTC CCGCTC(-3/-3)
195 GAGCGG
BsrI 332 CCAGT ACTGG(1/-1)
339 CCAGT
DpnII 915 GATC ^GATC
1352 GATC
EarI 988 CTCTTC CTCTTC(1/4)
838 GAAGAG
HphI 156 GGTGA GGTGA(8/7)
381 GGTGA
Ksp632I 988 CTCTTC CTCTTC(1/4)
838 GAAGAG
MboI 915 GATC ^GATC
1352 GATC
NcoI 185 CCATGG C^CATGG
377 CCATGG
NdeII 915 GATC ^GATC
1352 GATC
PaeR7I 118 CTCGAG C^TCGAG
559 CTCGAG
PstI 468 CTGCAG CTGCA^G
1069 CTGCAG
SacI 115 GAGCTC GAGCT^C
1117 GAGCTC
Sau3AI 915 GATC ^GATC
1352 GATC
SfaNI 127 GATGC GCATC(5/9)
654 GCATC
XhoI 118 CTCGAG C^TCGAG
559 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AluI Alw44I
ApaI ApaLI AspHI AvaI AvaII
BanI BanII BbeI BbrPI BbvI
BcnI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BssHII BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NgoMIV NlaIII NlaIV PleI PmaCI
PmlI PpuMI Sau96I ScrFI SfcI
SfoI SgrAI SmaI SmlI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XmaCI XmaI