##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AclI AcsI AflII
AgeI AhdI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BciVI BclI BfaI BfrI
BglII BlnI BsaBI BsaI BsiWI
BsmBI BsmI BspDI BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
CpoI DraI Eam1105I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MaeI
MamI MfeI MluI MseI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SbfI ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI Tru9I Tsp509I Tth111I Van91I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 1378 GTCTAC GT^[AC][GT]AC
AccIII 612 TCCGGA T^CCGGA
Alw44I 1348 GTGCAC G^TGCAC
ApaLI 1348 GTGCAC G^TGCAC
Asp700I 1326 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 29 GGCGCC GGCGC^C
BbrPI 84 CACGTG CAC^GTG
BbsI 1028 GAAGAC GAAGAC(2/6)
BbvCI 1519 GCTGAGG CCTCAGC(-5/-2)
BcgI 53 CGAGCACCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 588 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 588 GCTCAGC GC^T[ACGT]AGC
BpuAI 1028 GAAGAC GAAGAC(2/6)
BsaAI 84 CACGTG [CT]AC^GT[GA]
BsaWI 612 TCCGGA [AT]^CCGG[AT]
BseAI 612 TCCGGA T^CCGGA
BsgI 1496 CTGCAC GTGCAG(16/14)
Bsp1407I 386 TGTACA T^GTACA
BspEI 612 TCCGGA T^CCGGA
BspHI 482 TCATGA T^CATGA
BspLU11I 1448 ACATGT A^CATGT
BsrDI 1478 CATTGC GCAATG(2/0)
BsrGI 386 TGTACA T^GTACA
BstEII 1083 GGTGACC G^GT[ACGT]ACC
BstPI 1083 GGTGACC G^GT[ACGT]ACC
CelII 588 GCTCAGC GC^T[ACGT]AGC
DraIII 34 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1083 GGTGACC G^GT[ACGT]ACC
KasI 29 GGCGCC G^GCGCC
KspI 594 CCGCGG CCGC^GG
MroI 612 TCCGGA T^CCGGA
NarI 29 GGCGCC GG^CGCC
PaeR7I 273 CTCGAG C^TCGAG
PleI 286 GACTC GAGTC(4/5)
PmaCI 84 CACGTG CAC^GTG
PmlI 84 CACGTG CAC^GTG
RcaI 482 TCATGA T^CATGA
SacI 586 GAGCTC GAGCT^C
SacII 594 CCGCGG CCGC^GG
SfiI 740 GGCCCACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 29 GGCGCC GGC^GCC
SphI 1404 GCATGC GCATG^C
SspBI 386 TGTACA T^GTACA
TfiI 577 GAATC G^A[AT]TC
XhoI 273 CTCGAG C^TCGAG
XmnI 1326 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 595 CGCG CG^CG
621 CGCG
AcyI 29 GGCGCC G[GA]^CG[CT]C
48 GACGCC
AfaI 387 GTAC GT^AC
1446 GTAC
AflIII 83 ACACGT A^C[GA][CT]GT
1448 ACATGT
AocI 629 CCTCAGG CC^T[ACGT]AGG
1643 CCTGAGG
Aor51HI 559 AGCGCT AGC^GCT
1127 AGCGCT
BamHI 837 GGATCC G^GATCC
873 GGATCC
BsaHI 29 GGCGCC G[GA]^CG[CT]C
48 GACGCC
BspMI 176 ACCTGC ACCTGC(4/8)
1372 GCAGGT
BsrI 159 CCAGT ACTGG(1/-1)
376 CCAGT
BssSI 390 CACGAG CACGAG(-5/-1)
744 CACGAG
BstUI 595 CGCG CG^CG
621 CGCG
BstYI 837 GGATCC [GA]^GATC[CT]
873 GGATCC
Bsu36I 629 CCTCAGG CC^T[ACGT]AGG
1643 CCTGAGG
DrdI 432 GACGCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1165 GACCGCTACGTC
EarI 1330 CTCTTC CTCTTC(1/4)
1575 GAAGAG
Eco47III 559 AGCGCT AGC^GCT
1127 AGCGCT
Eco81I 629 CCTCAGG CC^T[ACGT]AGG
1643 CCTGAGG
EcoNI 1314 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1584 CCTGGGCAAGG
Hin1I 29 GGCGCC G[GA]^CG[CT]C
48 GACGCC
HinfI 286 GACTC G^A[ACGT]TC
577 GAATC
Ksp632I 1330 CTCTTC CTCTTC(1/4)
1575 GAAGAG
MflI 837 GGATCC [GA]^GATC[CT]
873 GGATCC
MvnI 595 CGCG CG^CG
621 CGCG
NspI 1404 GCATGC [GA]CATG^[CT]
1448 ACATGT
PpuMI 1151 GGGTCCT [GA]G^G[AT]CC[CT]
1465 GGGTCCC
RsaI 387 GTAC GT^AC
1446 GTAC
SfaNI 248 GCATC GCATC(5/9)
1706 GCATC
TaqI 146 TCGA T^CGA
274 TCGA
TspRI 160 CAGTG CA[GC]TG[ACGT][ACGT]^
1205 CAGTG
TthHB8I 146 TCGA T^CGA
274 TCGA
XcmI 191 CCAAGCTGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1196 CCAGCGAGACAGTGG
XhoII 837 GGATCC [GA]^GATC[CT]
873 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrBI BsrFI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PstI PvuII Sau3AI Sau96I ScrFI
SfcI SmlI StuI StyI TseI
Tsp45I