################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BsiWI BsmBI BsmI BspDI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 1378 GTCTAC GT^[AC][GT]AC AccIII 612 TCCGGA T^CCGGA Alw44I 1348 GTGCAC G^TGCAC ApaLI 1348 GTGCAC G^TGCAC Asp700I 1326 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 29 GGCGCC GGCGC^C BbrPI 84 CACGTG CAC^GTG BbsI 1028 GAAGAC GAAGAC(2/6) BbvCI 1519 GCTGAGG CCTCAGC(-5/-2) BcgI 53 CGAGCACCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 588 GCTCAGC GC^T[ACGT]AGC Bpu1102I 588 GCTCAGC GC^T[ACGT]AGC BpuAI 1028 GAAGAC GAAGAC(2/6) BsaAI 84 CACGTG [CT]AC^GT[GA] BsaWI 612 TCCGGA [AT]^CCGG[AT] BseAI 612 TCCGGA T^CCGGA BsgI 1496 CTGCAC GTGCAG(16/14) Bsp1407I 386 TGTACA T^GTACA BspEI 612 TCCGGA T^CCGGA BspHI 482 TCATGA T^CATGA BspLU11I 1448 ACATGT A^CATGT BsrDI 1478 CATTGC GCAATG(2/0) BsrGI 386 TGTACA T^GTACA BstEII 1083 GGTGACC G^GT[ACGT]ACC BstPI 1083 GGTGACC G^GT[ACGT]ACC CelII 588 GCTCAGC GC^T[ACGT]AGC DraIII 34 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 1083 GGTGACC G^GT[ACGT]ACC KasI 29 GGCGCC G^GCGCC KspI 594 CCGCGG CCGC^GG MroI 612 TCCGGA T^CCGGA NarI 29 GGCGCC GG^CGCC PaeR7I 273 CTCGAG C^TCGAG PleI 286 GACTC GAGTC(4/5) PmaCI 84 CACGTG CAC^GTG PmlI 84 CACGTG CAC^GTG RcaI 482 TCATGA T^CATGA SacI 586 GAGCTC GAGCT^C SacII 594 CCGCGG CCGC^GG SfiI 740 GGCCCACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 29 GGCGCC GGC^GCC SphI 1404 GCATGC GCATG^C SspBI 386 TGTACA T^GTACA TfiI 577 GAATC G^A[AT]TC XhoI 273 CTCGAG C^TCGAG XmnI 1326 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 595 CGCG CG^CG 621 CGCG AcyI 29 GGCGCC G[GA]^CG[CT]C 48 GACGCC AfaI 387 GTAC GT^AC 1446 GTAC AflIII 83 ACACGT A^C[GA][CT]GT 1448 ACATGT AocI 629 CCTCAGG CC^T[ACGT]AGG 1643 CCTGAGG Aor51HI 559 AGCGCT AGC^GCT 1127 AGCGCT BamHI 837 GGATCC G^GATCC 873 GGATCC BsaHI 29 GGCGCC G[GA]^CG[CT]C 48 GACGCC BspMI 176 ACCTGC ACCTGC(4/8) 1372 GCAGGT BsrI 159 CCAGT ACTGG(1/-1) 376 CCAGT BssSI 390 CACGAG CACGAG(-5/-1) 744 CACGAG BstUI 595 CGCG CG^CG 621 CGCG BstYI 837 GGATCC [GA]^GATC[CT] 873 GGATCC Bsu36I 629 CCTCAGG CC^T[ACGT]AGG 1643 CCTGAGG DrdI 432 GACGCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1165 GACCGCTACGTC EarI 1330 CTCTTC CTCTTC(1/4) 1575 GAAGAG Eco47III 559 AGCGCT AGC^GCT 1127 AGCGCT Eco81I 629 CCTCAGG CC^T[ACGT]AGG 1643 CCTGAGG EcoNI 1314 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1584 CCTGGGCAAGG Hin1I 29 GGCGCC G[GA]^CG[CT]C 48 GACGCC HinfI 286 GACTC G^A[ACGT]TC 577 GAATC Ksp632I 1330 CTCTTC CTCTTC(1/4) 1575 GAAGAG MflI 837 GGATCC [GA]^GATC[CT] 873 GGATCC MvnI 595 CGCG CG^CG 621 CGCG NspI 1404 GCATGC [GA]CATG^[CT] 1448 ACATGT PpuMI 1151 GGGTCCT [GA]G^G[AT]CC[CT] 1465 GGGTCCC RsaI 387 GTAC GT^AC 1446 GTAC SfaNI 248 GCATC GCATC(5/9) 1706 GCATC TaqI 146 TCGA T^CGA 274 TCGA TspRI 160 CAGTG CA[GC]TG[ACGT][ACGT]^ 1205 CAGTG TthHB8I 146 TCGA T^CGA 274 TCGA XcmI 191 CCAAGCTGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1196 CCAGCGAGACAGTGG XhoII 837 GGATCC [GA]^GATC[CT] 873 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AciI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PstI PvuII Sau3AI Sau96I ScrFI SfcI SmlI StuI StyI TseI Tsp45I