##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI BbeI
BbrPI BbsI BcgI BclI BfrI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsgI BsiEI BsiWI BspDI
BspEI BspLU11I BspMI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV FbaI FseI HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MunI MvnI
NarI NcoI NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcsI 630 AAATTT [GA]^AATT[CT]
AcyI 1608 GACGCC G[GA]^CG[CT]C
Alw44I 303 GTGCAC G^TGCAC
AlwNI 292 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 303 GTGCAC G^TGCAC
ApoI 630 AAATTT [GA]^AATT[CT]
AviII 660 TGCGCA TGC^GCA
BalI 515 TGGCCA TGG^CCA
BamHI 357 GGATCC G^GATCC
BciVI 1137 GTATCC GTATCC(6/5)
BglII 1095 AGATCT A^GATCT
BsaHI 1608 GACGCC G[GA]^CG[CT]C
BsaWI 86 TCCGGT [AT]^CCGG[AT]
BseRI 2003 CTCCTC GAGGAG(10/8)
Bsp1407I 1382 TGTACA T^GTACA
BspHI 197 TCATGA T^CATGA
BsrBI 888 GAGCGG CCGCTC(-3/-3)
BsrFI 1748 GCCGGC [GA]^CCGG[CT]
BsrGI 1382 TGTACA T^GTACA
BstXI 1303 CCACTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1748 GCCGGC [GA]^CCGG[CT]
DraI 1120 TTTAAA TTT^AAA
DrdI 1342 GACAATACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 913 ATGCAT ATGCA^T
FspI 660 TGCGCA TGC^GCA
Hin1I 1608 GACGCC G[GA]^CG[CT]C
MluNI 515 TGGCCA TGG^CCA
MscI 515 TGGCCA TGG^CCA
NaeI 1748 GCCGGC GCC^GGC
NgoMIV 1748 GCCGGC G^CCGGC
NsiI 913 ATGCAT ATGCA^T
NspI 911 ACATGC [GA]CATG^[CT]
PaeR7I 1846 CTCGAG C^TCGAG
PflMI 1738 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1665 AGGACCT [GA]G^G[AT]CC[CT]
PstI 904 CTGCAG CTGCA^G
RcaI 197 TCATGA T^CATGA
SacI 1790 GAGCTC GAGCT^C
SfiI 173 GGCCCCTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1846 CTCGAG C^T[CT][GA]AG
SspBI 1382 TGTACA T^GTACA
Van91I 1738 CCAACTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2007 TCTAGA T^CTAGA
XcmI 15 CCAGAGACCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1846 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AocI 285 CCTGAGG CC^T[ACGT]AGG
838 CCTCAGG
AspHI 303 GTGCAC G[AT]GC[AT]^C
1790 GAGCTC
AvrII 1489 CCTAGG C^CTAGG
1919 CCTAGG
BanI 243 GGTGCC G^G[CT][GA]CC
844 GGCACC
BanII 49 GGGCTC G[GA]GC[CT]^C
1790 GAGCTC
BbvCI 68 CCTCAGC CCTCAGC(-5/-2)
79 CCTCAGC
BcnI 102 CCGGG CC^[GC]GG
164 CCGGG
BglI 37 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
174 GCCCCTCGGGC
BlnI 1489 CCTAGG C^CTAGG
1919 CCTAGG
BsiHKAI 303 GTGCAC G[AT]GC[AT]^C
1790 GAGCTC
BsmBI 250 GAGACG CGTCTC(1/5)
1606 GAGACG
BsmI 480 GCATTC GAATGC(1/-1)
738 GCATTC
BstAPI 291 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
906 GCAGTACATGC
Bsu36I 285 CCTGAGG CC^T[ACGT]AGG
838 CCTCAGG
EarI 376 GAAGAG CTCTTC(1/4)
1389 GAAGAG
Eco81I 285 CCTGAGG CC^T[ACGT]AGG
838 CCTCAGG
HaeII 4 GGCGCT [GA]GCGC^[CT]
447 AGCGCC
HgaI 261 GACGC GACGC(5/10)
1608 GACGC
HincII 1449 GTCAAC GT[CT]^[GA]AC
1824 GTCAAC
HindII 1449 GTCAAC GT[CT]^[GA]AC
1824 GTCAAC
HphI 1534 GGTGA GGTGA(8/7)
947 TCACC
Ksp632I 376 GAAGAG CTCTTC(1/4)
1389 GAAGAG
NciI 102 CCGGG CC^[GC]GG
164 CCGGG
NdeI 392 CATATG CA^TATG
664 CATATG
PleI 643 GAGTC GAGTC(4/5)
823 GAGTC
TaqI 1338 TCGA T^CGA
1847 TCGA
Tsp45I 1277 GTGAC ^GT[GC]AC
1950 GTGAC
TthHB8I 1338 TCGA T^CGA
1847 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BbvI BfaI BmyI BpmI
BsaI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII