##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AviII AvrII BbeI BbrPI
BcgI BfaI BfrI BglI BglII
BlnI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BsrBI BsrDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FseI FspI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MluI MroI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AfaI 142 GTAC GT^AC
AhdI 2049 GACTAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1132 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 430 GGGCCC GGGCC^C
Asp700I 808 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2049 GACTAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 296 GAGCTC G[AT]GC[AT]^C
AspI 1942 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1813 TGGCCA TGG^CCA
BamHI 424 GGATCC G^GATCC
BanI 512 GGTGCC G^G[CT][GA]CC
BbvCI 1924 CCTCAGC CCTCAGC(-5/-2)
BciVI 765 GGATAC GTATCC(6/5)
BclI 484 TGATCA T^GATCA
BlpI 118 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 118 GCTGAGC GC^T[ACGT]AGC
BseRI 1112 GAGGAG GAGGAG(10/8)
BsiHKAI 296 GAGCTC G[AT]GC[AT]^C
Bsp1407I 141 TGTACA T^GTACA
BspMI 747 ACCTGC ACCTGC(4/8)
BsrGI 141 TGTACA T^GTACA
BstAPI 352 GCAGCATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 118 GCTGAGC GC^T[ACGT]AGC
CfoI 43 GCGC GCG^C
DraII 496 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 1542 CCATGG C^C[GA][CT]GG
Eam1105I 2049 GACTAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 496 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1327 GAATTC G^AATTC
EcoT22I 2095 ATGCAT ATGCA^T
FbaI 484 TGATCA T^GATCA
HaeII 42 GGCGCT [GA]GCGC^[CT]
HhaI 43 GCGC GCG^C
HinP1I 43 GCGC G^CGC
MfeI 870 CAATTG C^AATTG
MluNI 1813 TGGCCA TGG^CCA
MscI 1813 TGGCCA TGG^CCA
MseI 900 TTAA T^TAA
MunI 870 CAATTG C^AATTG
NcoI 1542 CCATGG C^CATGG
NdeI 1878 CATATG CA^TATG
NsiI 2095 ATGCAT ATGCA^T
PaeR7I 1696 CTCGAG C^TCGAG
PflFI 1942 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 6 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 496 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 142 GTAC GT^AC
SacI 296 GAGCTC GAGCT^C
SapI 826 GAAGAGC GCTCTTC(1/4)
SexAI 174 ACCTGGT A^CC[AT]GGT
SfcI 166 CTACAG C^T[GA][CT]AG
SspBI 141 TGTACA T^GTACA
TfiI 2009 GATTC G^A[AT]TC
Tru9I 900 TTAA T^TAA
Tth111I 1942 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 6 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 6 CCAGCTGGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1696 CTCGAG C^TCGAG
XmnI 808 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AocI 500 CCTGAGG CC^T[ACGT]AGG
650 CCTGAGG
AvaI 856 CTCGGG C^[CT]CG[GA]G
1696 CTCGAG
BsaAI 788 TACGTG [CT]AC^GT[GA]
1040 TACGTG
BsaWI 903 ACCGGA [AT]^CCGG[AT]
941 TCCGGT
BsmFI 438 GGGAC GGGAC(10/14)
2041 GGGAC
BsmI 71 GCATTC GAATGC(1/-1)
2097 GCATTC
BsoBI 856 CTCGGG C^[CT]CG[GA]G
1696 CTCGAG
BspLU11I 957 ACATGT A^CATGT
1713 ACATGT
BsrFI 268 ACCGGC [GA]^CCGG[CT]
1667 GCCGGT
Bsu36I 500 CCTGAGG CC^T[ACGT]AGG
650 CCTGAGG
Cfr10I 268 ACCGGC [GA]^CCGG[CT]
1667 GCCGGT
EaeI 1477 TGGCCG [CT]^GGCC[GA]
1813 TGGCCA
Eco81I 500 CCTGAGG CC^T[ACGT]AGG
650 CCTGAGG
EcoT14I 1542 CCATGG C^C[AT][AT]GG
1816 CCAAGG
SfaNI 154 GATGC GCATC(5/9)
1230 GATGC
StyI 1542 CCATGG C^C[AT][AT]GG
1816 CCAAGG
TaqI 306 TCGA T^CGA
1697 TCGA
TthHB8I 306 TCGA T^CGA
1697 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwI
ApoI AvaII BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaI
BsaJI BsgI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII Sau3AI Sau96I
ScrFI SmlI TseI Tsp45I Tsp509I
TspRI XhoII