##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI BamHI
BbrPI BbsI BfrI BglII BpuAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrDI BssSI Bst1107I BstAPI
BstBI BstYI BstZ17I Bsu36I ClaI
CpoI DraIII DrdI Eam1105I Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI HpaI KpnI KspI MamI
MfeI MflI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SnaBI SpeI Sse8387I SspI
SwaI Van91I XbaI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 59 GGCGCC G[GA]^CG[CT]C
ApaI 128 GGGCCC GGGCC^C
AspI 1656 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1864 TGCGCA TGC^GCA
AvrII 2055 CCTAGG C^CTAGG
BalI 1879 TGGCCA TGG^CCA
BbeI 59 GGCGCC GGCGC^C
BcgI 583 GCAAGATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 470 GGATAC GTATCC(6/5)
BclI 1264 TGATCA T^GATCA
BfaI 2056 CTAG C^TAG
BlnI 2055 CCTAGG C^CTAGG
BlpI 1992 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1992 GCTCAGC GC^T[ACGT]AGC
BsaAI 1029 TACGTG [CT]AC^GT[GA]
BsaHI 59 GGCGCC G[GA]^CG[CT]C
BsiEI 73 CGGCCG CG[GA][CT]^CG
Bsp1407I 1093 TGTACA T^GTACA
BsrGI 1093 TGTACA T^GTACA
BstXI 467 CCAGGATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1992 GCTCAGC GC^T[ACGT]AGC
DraI 2184 TTTAAA TTT^AAA
EagI 73 CGGCCG C^GGCCG
EclXI 73 CGGCCG C^GGCCG
Eco52I 73 CGGCCG C^GGCCG
FbaI 1264 TGATCA T^GATCA
FspI 1864 TGCGCA TGC^GCA
Hin1I 59 GGCGCC G[GA]^CG[CT]C
HincII 2046 GTTGAC GT[CT]^[GA]AC
HindII 2046 GTTGAC GT[CT]^[GA]AC
KasI 59 GGCGCC G^GCGCC
MaeI 2056 CTAG C^TAG
MluNI 1879 TGGCCA TGG^CCA
MscI 1879 TGGCCA TGG^CCA
NarI 59 GGCGCC GG^CGCC
PflFI 1656 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1410 GAGTC GAGTC(4/5)
PpuMI 1698 AGGTCCC [GA]G^G[AT]CC[CT]
SapI 495 GAAGAGC GCTCTTC(1/4)
SfoI 59 GGCGCC GGC^GCC
SgrAI 61 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 2163 CTTGAG C^T[CT][GA]AG
SspBI 1093 TGTACA T^GTACA
StuI 541 AGGCCT AGG^CCT
TaqI 216 TCGA T^CGA
Tth111I 1656 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 216 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcsI 1269 AAATTC [GA]^AATT[CT]
2181 AAATTT
AlwI 1667 GGATC GGATC(4/5)
2006 GGATC
ApoI 1269 AAATTC [GA]^AATT[CT]
2181 AAATTT
AvaI 668 CCCGGG C^[CT]CG[GA]G
802 CCCGGG
BbvCI 1196 CCTCAGC CCTCAGC(-5/-2)
570 GCTGAGG
BglI 1139 GCCCAAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1424 GCCCTCCGGGC
BsmBI 1650 GAGACG CGTCTC(1/5)
1731 GAGACG
BsoBI 668 CCCGGG C^[CT]CG[GA]G
802 CCCGGG
BsrBI 656 CCGCTC CCGCTC(-3/-3)
1005 CCGCTC
BsrFI 62 GCCGGC [GA]^CCGG[CT]
1473 GCCGGC
Cfr10I 62 GCCGGC [GA]^CCGG[CT]
1473 GCCGGC
EarI 1572 CTCTTC CTCTTC(1/4)
495 GAAGAG
HgaI 999 GACGC GACGC(5/10)
380 GCGTC
HindIII 285 AAGCTT A^AGCTT
727 AAGCTT
Ksp632I 1572 CTCTTC CTCTTC(1/4)
495 GAAGAG
MseI 2185 TTAA T^TAA
2198 TTAA
NaeI 62 GCCGGC GCC^GGC
1473 GCCGGC
NcoI 1822 CCATGG C^CATGG
2062 CCATGG
NgoMIV 62 GCCGGC G^CCGGC
1473 GCCGGC
NspI 1465 GCATGC [GA]CATG^[CT]
1867 GCATGC
SacI 1596 GAGCTC GAGCT^C
1740 GAGCTC
SmaI 668 CCCGGG CCC^GGG
802 CCCGGG
SphI 1465 GCATGC GCATG^C
1867 GCATGC
Tru9I 2185 TTAA T^TAA
2198 TTAA
XmaCI 668 CCCGGG C^CCGGG
802 CCCGGG
XmaI 668 CCCGGG C^CCGGG
802 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssHII BssKI
BstEII BstF5I BstNI BstPI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp45I Tsp509I TspRI XcmI