##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwI Aor51HI
ApaLI AscI Asp700I AspEI AspI
AvaI AvrII BamHI BbsI BcgI
BclI BfrI BglII BlnI BpmI
BpuAI BsaBI BseAI BsgI BsiWI
BsoBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssSI Bst1107I BstEII
BstPI BstXI BstYI BstZ17I ClaI
CpoI DraIII DrdI Eam1105I Eco47III
EcoNI EcoO65I EcoRV FbaI FseI
HindIII HpaI MamI MfeI MflI
MluI MroI MunI NcoI NruI
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SgrAI SmaI
SmlI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AseI 2450 ATTAAT AT^TAAT
AsnI 2450 ATTAAT AT^TAAT
AviII 244 TGCGCA TGC^GCA
BalI 1419 TGGCCA TGG^CCA
BbeI 303 GGCGCC GGCGC^C
BbrPI 110 CACGTG CAC^GTG
BbvCI 277 GCTGAGG CCTCAGC(-5/-2)
BciVI 2000 GTATCC GTATCC(6/5)
BglI 171 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 620 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 620 GCTCAGC GC^T[ACGT]AGC
BsaAI 110 CACGTG [CT]AC^GT[GA]
BsaI 2532 GGTCTC GGTCTC(1/5)
BspMI 760 GCAGGT ACCTGC(4/8)
BsrDI 1782 CATTGC GCAATG(2/0)
BsrFI 229 GCCGGC [GA]^CCGG[CT]
BstAPI 874 GCAGATATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 802 TTCGAA TT^CGAA
CelII 620 GCTCAGC GC^T[ACGT]AGC
Cfr10I 229 GCCGGC [GA]^CCGG[CT]
DpnII 1045 GATC ^GATC
DraII 1901 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1901 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 899 CCAAGG C^C[AT][AT]GG
FspI 244 TGCGCA TGC^GCA
KasI 303 GGCGCC G^GCGCC
KspI 285 CCGCGG CCGC^GG
MboI 1045 GATC ^GATC
MluNI 1419 TGGCCA TGG^CCA
MscI 1419 TGGCCA TGG^CCA
NaeI 229 GCCGGC GCC^GGC
NarI 303 GGCGCC GG^CGCC
NdeII 1045 GATC ^GATC
NgoMIV 229 GCCGGC G^CCGGC
NheI 909 GCTAGC G^CTAGC
NotI 1 GCGGCCGC GC^GGCCGC
NspV 802 TTCGAA TT^CGAA
PflMI 1126 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 110 CACGTG CAC^GTG
PmlI 110 CACGTG CAC^GTG
PshBI 2450 ATTAAT AT^TAAT
SacII 285 CCGCGG CCGC^GG
Sau3AI 1045 GATC ^GATC
SfoI 303 GGCGCC GGC^GCC
SfuI 802 TTCGAA TT^CGAA
SpeI 1561 ACTAGT A^CTAGT
StyI 899 CCAAGG C^C[AT][AT]GG
Van91I 1126 CCATTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 8 CCACGCGACGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 49 GGTACC G^GTACC
528 GGTACC
AlwNI 248 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1432 CAGGAGCTG
AocI 197 CCTGAGG CC^T[ACGT]AGG
1714 CCTGAGG
ApaI 25 GGGCCC GGGCC^C
289 GGGCCC
Asp718I 49 GGTACC G^GTACC
528 GGTACC
AspHI 618 GTGCTC G[AT]GC[AT]^C
649 GAGCAC
AvaII 1329 GGTCC G^G[AT]CC
2266 GGACC
BcnI 348 CCCGG CC^[GC]GG
378 CCCGG
BsaWI 33 ACCGGA [AT]^CCGG[AT]
653 ACCGGA
BseRI 400 GAGGAG GAGGAG(10/8)
1608 GAGGAG
BsiEI 2 CGGCCG CG[GA][CT]^CG
231 CGGCCG
BsiHKAI 618 GTGCTC G[AT]GC[AT]^C
649 GAGCAC
BsmBI 43 CGTCTC CGTCTC(1/5)
316 GAGACG
BsmI 1062 GAATGC GAATGC(1/-1)
840 GCATTC
BspLU11I 663 ACATGT A^CATGT
1981 ACATGT
Bsu36I 197 CCTGAGG CC^T[ACGT]AGG
1714 CCTGAGG
EagI 2 CGGCCG C^GGCCG
231 CGGCCG
EarI 1030 GAAGAG CTCTTC(1/4)
1663 GAAGAG
EclXI 2 CGGCCG C^GGCCG
231 CGGCCG
Eco52I 2 CGGCCG C^GGCCG
231 CGGCCG
Eco81I 197 CCTGAGG CC^T[ACGT]AGG
1714 CCTGAGG
EcoRI 724 GAATTC G^AATTC
2102 GAATTC
EcoT22I 1069 ATGCAT ATGCA^T
2195 ATGCAT
HincII 772 GTTGAC GT[CT]^[GA]AC
2118 GTCAAC
HindII 772 GTTGAC GT[CT]^[GA]AC
2118 GTCAAC
KpnI 49 GGTACC GGTAC^C
528 GGTACC
Ksp632I 1030 GAAGAG CTCTTC(1/4)
1663 GAAGAG
MaeII 111 ACGT A^CGT
1392 ACGT
NciI 348 CCCGG CC^[GC]GG
378 CCCGG
NdeI 2192 CATATG CA^TATG
2261 CATATG
NsiI 1069 ATGCAT ATGCA^T
2195 ATGCAT
PstI 758 CTGCAG CTGCA^G
1457 CTGCAG
PvuII 1617 CAGCTG CAG^CTG
2240 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI ApoI BanI BanII
BbvI BfaI BmyI BsaHI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DraI DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NlaIII
NlaIV NspI PleI RsaI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I