##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsiWI
BspDI BspHI BsrBI BsrFI BssHII
BssSI Bst1107I BstBI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KspI MamI MfeI MluI MunI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SapI SbfI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 1460 GGTACC G^GTACC
AccI 369 GTCGAC GT^[AC][GT]AC
AccII 113 CGCG CG^CG
AccIII 125 TCCGGA T^CCGGA
AcsI 1612 AAATTT [GA]^AATT[CT]
AflIII 1105 ACATGT A^C[GA][CT]GT
AlwNI 271 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1211 CCTGAGG CC^T[ACGT]AGG
ApoI 1612 AAATTT [GA]^AATT[CT]
Asp718I 1460 GGTACC G^GTACC
BlpI 1307 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1307 GCTGAGC GC^T[ACGT]AGC
BsaI 1230 GAGACC GGTCTC(1/5)
BsaWI 125 TCCGGA [AT]^CCGG[AT]
BseAI 125 TCCGGA T^CCGGA
BsgI 1534 GTGCAG GTGCAG(16/14)
BsiEI 548 CGGCCG CG[GA][CT]^CG
BsmBI 106 GAGACG CGTCTC(1/5)
BsmI 359 GAATGC GAATGC(1/-1)
Bsp1407I 1108 TGTACA T^GTACA
BspEI 125 TCCGGA T^CCGGA
BspLU11I 1105 ACATGT A^CATGT
BsrDI 323 CATTGC GCAATG(2/0)
BsrGI 1108 TGTACA T^GTACA
BstAPI 1064 GCACTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1254 GGTGACC G^GT[ACGT]ACC
BstPI 1254 GGTGACC G^GT[ACGT]ACC
BstUI 113 CGCG CG^CG
BstYI 905 GGATCT [GA]^GATC[CT]
Bsu36I 1211 CCTGAGG CC^T[ACGT]AGG
CelII 1307 GCTGAGC GC^T[ACGT]AGC
CfoI 112 GCGC GCG^C
EagI 548 CGGCCG C^GGCCG
EarI 348 GAAGAG CTCTTC(1/4)
EclXI 548 CGGCCG C^GGCCG
Eco52I 548 CGGCCG C^GGCCG
Eco81I 1211 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1254 GGTGACC G^GT[ACGT]ACC
HhaI 112 GCGC GCG^C
HinP1I 112 GCGC G^CGC
KpnI 1460 GGTACC GGTAC^C
Ksp632I 348 GAAGAG CTCTTC(1/4)
MaeII 75 ACGT A^CGT
MflI 905 GGATCT [GA]^GATC[CT]
MroI 125 TCCGGA T^CCGGA
MseI 1646 TTAA T^TAA
MvnI 113 CGCG CG^CG
NdeI 1476 CATATG CA^TATG
PpuMI 1078 GGGACCT [GA]G^G[AT]CC[CT]
SacI 318 GAGCTC GAGCT^C
SalI 369 GTCGAC G^TCGAC
SfiI 253 GGCCCCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 442 CCCGGG CCC^GGG
SmlI 712 CTCAAG C^T[CT][GA]AG
SphI 664 GCATGC GCATG^C
SspBI 1108 TGTACA T^GTACA
TaqI 370 TCGA T^CGA
Tru9I 1646 TTAA T^TAA
TthHB8I 370 TCGA T^CGA
XhoII 905 GGATCT [GA]^GATC[CT]
XmaCI 442 CCCGGG C^CCGGG
XmaI 442 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
ApaI 1277 GGGCCC GGGCC^C
1403 GGGCCC
Asp700I 134 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
941 GAAGACCTTC
AspHI 318 GAGCTC G[AT]GC[AT]^C
1321 GAGCAC
AvaI 101 CTCGGG C^[CT]CG[GA]G
442 CCCGGG
AvaII 482 GGTCC G^G[AT]CC
1079 GGACC
BalI 227 TGGCCA TGG^CCA
505 TGGCCA
BbsI 941 GAAGAC GAAGAC(2/6)
1218 GAAGAC
BfaI 1408 CTAG C^TAG
1587 CTAG
BglI 254 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
710 GCCTCAAGGGC
BpmI 727 CTGGAG CTGGAG(16/14)
1227 CTGGAG
BpuAI 941 GAAGAC GAAGAC(2/6)
1218 GAAGAC
BsiHKAI 318 GAGCTC G[AT]GC[AT]^C
1321 GAGCAC
BsoBI 101 CTCGGG C^[CT]CG[GA]G
442 CCCGGG
BspMI 862 ACCTGC ACCTGC(4/8)
1081 ACCTGC
HincII 369 GTCGAC GT[CT]^[GA]AC
582 GTCAAC
HindII 369 GTCGAC GT[CT]^[GA]AC
582 GTCAAC
MaeI 1408 CTAG C^TAG
1587 CTAG
MluNI 227 TGGCCA TGG^CCA
505 TGGCCA
MscI 227 TGGCCA TGG^CCA
505 TGGCCA
MslI 1133 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1377 CACTACGATG
MspA1I 27 CCGCTG C[AC]G^C[GT]G
416 CAGCGG
NcoI 811 CCATGG C^CATGG
1432 CCATGG
PflMI 1281 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1394 CCAGTACATGG
PstI 168 CTGCAG CTGCA^G
1027 CTGCAG
ScaI 856 AGTACT AGT^ACT
1641 AGTACT
SfcI 168 CTGCAG C^T[GA][CT]AG
1027 CTGCAG
SspI 1123 AATATT AAT^ATT
1601 AATATT
TfiI 330 GAATC G^A[AT]TC
953 GAATC
Van91I 1281 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1394 CCAGTACATGG
XcmI 334 CCAAGAAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
811 CCATGGTTCGTCTGG
XmnI 134 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
941 GAAGACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI BanI
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EaeI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StuI StyI
TseI Tsp45I Tsp509I TspRI