##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BbrPI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FbaI FseI FspI
HgaI HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MslI MunI MvnI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 20 GGCGCC G[GA]^CG[CT]C
AocI 887 CCTGAGG CC^T[ACGT]AGG
ApaI 596 GGGCCC GGGCC^C
AspI 893 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 20 GGCGCC GGCGC^C
BbsI 659 GTCTTC GAAGAC(2/6)
BbvCI 2 GCTGAGG CCTCAGC(-5/-2)
BciVI 512 GGATAC GTATCC(6/5)
BglII 456 AGATCT A^GATCT
BpuAI 659 GTCTTC GAAGAC(2/6)
BsaHI 20 GGCGCC G[GA]^CG[CT]C
BspHI 1135 TCATGA T^CATGA
BstAPI 710 GCAAAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1388 CCAGCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 887 CCTGAGG CC^T[ACGT]AGG
DraI 1971 TTTAAA TTT^AAA
EaeI 286 TGGCCG [CT]^GGCC[GA]
Eco81I 887 CCTGAGG CC^T[ACGT]AGG
EcoNI 1580 CCTTAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1507 GAATTC G^AATTC
EcoT22I 1608 ATGCAT ATGCA^T
HaeII 20 GGCGCC [GA]GCGC^[CT]
Hin1I 20 GGCGCC G[GA]^CG[CT]C
HincII 449 GTTGAC GT[CT]^[GA]AC
HindII 449 GTTGAC GT[CT]^[GA]AC
KasI 20 GGCGCC G^GCGCC
MaeII 1217 ACGT A^CGT
NaeI 17 GCCGGC GCC^GGC
NarI 20 GGCGCC GG^CGCC
NgoMIV 17 GCCGGC G^CCGGC
NsiI 1608 ATGCAT ATGCA^T
PflFI 893 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1729 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 987 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1098 CTGCAG CTGCA^G
RcaI 1135 TCATGA T^CATGA
SapI 12 GAAGAGC GCTCTTC(1/4)
SfcI 1098 CTGCAG C^T[GA][CT]AG
SfoI 20 GGCGCC GGC^GCC
SmaI 1304 CCCGGG CCC^GGG
SphI 1103 GCATGC GCATG^C
Tth111I 893 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1729 CCAAGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1698 CCAGCTGGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1304 CCCGGG C^CCGGG
XmaI 1304 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 1507 GAATTC [GA]^AATT[CT]
1953 GAATTT
ApoI 1507 GAATTC [GA]^AATT[CT]
1953 GAATTT
AvaII 988 GGACC G^G[AT]CC
1901 GGTCC
BfaI 530 CTAG C^TAG
1813 CTAG
BsaI 1628 GAGACC GGTCTC(1/5)
1750 GAGACC
BspMI 879 ACCTGC ACCTGC(4/8)
1791 GCAGGT
BsrFI 17 GCCGGC [GA]^CCGG[CT]
84 ACCGGC
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
84 ACCGGC
EarI 12 GAAGAG CTCTTC(1/4)
786 GAAGAG
FauI 127 GCGGG CCCGC(4/6)
985 GCGGG
HphI 1232 TCACC GGTGA(8/7)
1458 TCACC
Ksp632I 12 GAAGAG CTCTTC(1/4)
786 GAAGAG
MaeI 530 CTAG C^TAG
1813 CTAG
NdeI 807 CATATG CA^TATG
1943 CATATG
NspI 1103 GCATGC [GA]CATG^[CT]
1853 ACATGC
PleI 857 GACTC GAGTC(4/5)
383 GAGTC
SacI 255 GAGCTC GAGCT^C
318 GAGCTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII