##################################
# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvrII BamHI
BanII BbeI BcgI BclI BfrI
BglII BlnI BsaBI BsaI BsiHKAI
BsiWI BsmAI BsmBI Bsp1407I BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HgaI KasI
KpnI MamI MfeI MflI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PmeI PshBI PvuI
RcaI RsrII SacI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp509I Tth111I XbaI
XcmI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1136 GACGTC GACGT^C
AccI 1169 GTCGAC GT^[AC][GT]AC
AclI 223 AACGTT AA^CGTT
AcyI 1136 GACGTC G[GA]^CG[CT]C
AflIII 831 ACGCGT A^C[GA][CT]GT
AlwI 639 GGATC GGATC(4/5)
Asp700I 1027 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 785 CTCGAG C^[CT]CG[GA]G
AviII 570 TGCGCA TGC^GCA
BbrPI 497 CACGTG CAC^GTG
BbvCI 1826 GCTGAGG CCTCAGC(-5/-2)
BciVI 406 GTATCC GTATCC(6/5)
BlpI 121 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 121 GCTGAGC GC^T[ACGT]AGC
BsaHI 1136 GACGTC G[GA]^CG[CT]C
BsiEI 1171 CGACCG CG[GA][CT]^CG
BsmI 150 GCATTC GAATGC(1/-1)
BsoBI 785 CTCGAG C^[CT]CG[GA]G
BspDI 419 ATCGAT AT^CGAT
BspMI 1203 GCAGGT ACCTGC(4/8)
CelII 121 GCTGAGC GC^T[ACGT]AGC
ClaI 419 ATCGAT AT^CGAT
DraI 355 TTTAAA TTT^AAA
DrdI 683 GACTACAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 568 CCTGCGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1974 CCTTGG C^C[AT][AT]GG
FspI 570 TGCGCA TGC^GCA
HaeII 1635 AGCGCC [GA]GCGC^[CT]
Hin1I 1136 GACGTC G[GA]^CG[CT]C
HindIII 1010 AAGCTT A^AGCTT
HpaI 197 GTTAAC GTT^AAC
KspI 479 CCGCGG CCGC^GG
MluI 831 ACGCGT A^CGCGT
MslI 1645 CATTATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 522 GCCGGC GCC^GGC
NgoMIV 522 GCCGGC G^CCGGC
PaeR7I 785 CTCGAG C^TCGAG
PflMI 630 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 497 CACGTG CAC^GTG
PmlI 497 CACGTG CAC^GTG
Psp1406I 223 AACGTT AA^CGTT
PstI 1370 CTGCAG CTGCA^G
SacII 479 CCGCGG CCGC^GG
SalI 1169 GTCGAC G^TCGAC
ScaI 1272 AGTACT AGT^ACT
StyI 1974 CCTTGG C^C[AT][AT]GG
Van91I 630 CCATCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 785 CTCGAG C^TCGAG
XmnI 1027 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccIII 303 TCCGGA T^CCGGA
312 TCCGGA
AlwNI 1488 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1601 CAGCGGCTG
BalI 696 TGGCCA TGG^CCA
1641 TGGCCA
BbsI 968 GAAGAC GAAGAC(2/6)
578 GTCTTC
BfaI 13 CTAG C^TAG
1840 CTAG
BpuAI 968 GAAGAC GAAGAC(2/6)
578 GTCTTC
BsaAI 497 CACGTG [CT]AC^GT[GA]
1211 TACGTG
BsaWI 303 TCCGGA [AT]^CCGG[AT]
312 TCCGGA
BseAI 303 TCCGGA T^CCGGA
312 TCCGGA
BsmFI 432 GGGAC GGGAC(10/14)
1104 GGGAC
BspEI 303 TCCGGA T^CCGGA
312 TCCGGA
DpnII 640 GATC ^GATC
1687 GATC
DsaI 479 CCGCGG C^C[GA][CT]GG
1263 CCGTGG
MaeI 13 CTAG C^TAG
1840 CTAG
MboI 640 GATC ^GATC
1687 GATC
MluNI 696 TGGCCA TGG^CCA
1641 TGGCCA
MroI 303 TCCGGA T^CCGGA
312 TCCGGA
MscI 696 TGGCCA TGG^CCA
1641 TGGCCA
NdeII 640 GATC ^GATC
1687 GATC
PshAI 1189 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1888 GACCAGAGTC
SapI 328 GAAGAGC GCTCTTC(1/4)
1535 GAAGAGC
Sau3AI 640 GATC ^GATC
1687 GATC
SmlI 47 CTTGAG C^T[CT][GA]AG
785 CTCGAG
StuI 43 AGGCCT AGG^CCT
2014 AGGCCT
TfiI 2033 GATTC G^A[AT]TC
2148 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BanI BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboII MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau96I ScrFI SfaNI SfcI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I