##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbeI BcgI
BciVI BclI BfrI BglI BlnI
BsaHI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BsrBI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI Hin1I
HpaI KasI MluI MroI NarI
NdeI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI Tth111I Van91I XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 2026 GGTACC G^GTACC
AflIII 523 ACATGT A^C[GA][CT]GT
Aor51HI 869 AGCGCT AGC^GCT
ApaI 1714 GGGCCC GGGCC^C
Asp718I 2026 GGTACC G^GTACC
AspHI 2380 GTGCTC G[AT]GC[AT]^C
BamHI 1909 GGATCC G^GATCC
BbrPI 1866 CACGTG CAC^GTG
BbvCI 1739 CCTCAGC CCTCAGC(-5/-2)
BglII 815 AGATCT A^GATCT
BlpI 319 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 319 GCTCAGC GC^T[ACGT]AGC
BsaAI 1866 CACGTG [CT]AC^GT[GA]
BsaBI 156 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 137 CGGTCG CG[GA][CT]^CG
BsiHKAI 2380 GTGCTC G[AT]GC[AT]^C
BsmI 2436 GAATGC GAATGC(1/-1)
Bsp1407I 526 TGTACA T^GTACA
BspHI 467 TCATGA T^CATGA
BspLU11I 523 ACATGT A^CATGT
BspMI 22 ACCTGC ACCTGC(4/8)
BsrGI 526 TGTACA T^GTACA
BssHII 10 GCGCGC G^CGCGC
BstXI 1259 CCAGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 319 GCTCAGC GC^T[ACGT]AGC
DraI 1642 TTTAAA TTT^AAA
DraIII 1981 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 869 AGCGCT AGC^GCT
EcoNI 2411 CCTGTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 371 GAATTC G^AATTC
HincII 1307 GTCGAC GT[CT]^[GA]AC
HindII 1307 GTCGAC GT[CT]^[GA]AC
HindIII 1635 AAGCTT A^AGCTT
KpnI 2026 GGTACC GGTAC^C
MamI 156 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 253 CAATTG C^AATTG
MunI 253 CAATTG C^AATTG
NaeI 179 GCCGGC GCC^GGC
NgoMIV 179 GCCGGC G^CCGGC
NheI 248 GCTAGC G^CTAGC
PaeR7I 68 CTCGAG C^TCGAG
PmaCI 1866 CACGTG CAC^GTG
PmlI 1866 CACGTG CAC^GTG
PpuMI 1750 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 2423 CTGCAG CTGCA^G
RcaI 467 TCATGA T^CATGA
SalI 1307 GTCGAC G^TCGAC
SapI 603 GCTCTTC GCTCTTC(1/4)
ScaI 1318 AGTACT AGT^ACT
SmaI 830 CCCGGG CCC^GGG
SspBI 526 TGTACA T^GTACA
SspI 1213 AATATT AAT^ATT
StuI 2419 AGGCCT AGG^CCT
XbaI 1059 TCTAGA T^CTAGA
XcmI 1606 CCAGTTGCACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 68 CTCGAG C^TCGAG
XmaCI 830 CCCGGG C^CCGGG
XmaI 830 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 1307 GTCGAC GT^[AC][GT]AC
2159 GTAGAC
AlwNI 322 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
846 CAGCATCTG
BalI 1901 TGGCCA TGG^CCA
1977 TGGCCA
BanI 726 GGTGCC G^G[CT][GA]CC
2026 GGTACC
BfaI 249 CTAG C^TAG
1060 CTAG
BsgI 1863 CTGCAC GTGCAG(16/14)
2341 CTGCAC
BsrDI 841 CATTGC GCAATG(2/0)
1994 CATTGC
BssSI 339 CTCGTG CACGAG(-5/-1)
1126 CTCGTG
HaeII 669 GGCGCT [GA]GCGC^[CT]
869 AGCGCT
KspI 41 CCGCGG CCGC^GG
83 CCGCGG
MaeI 249 CTAG C^TAG
1060 CTAG
MaeII 301 ACGT A^CGT
1867 ACGT
MluNI 1901 TGGCCA TGG^CCA
1977 TGGCCA
MscI 1901 TGGCCA TGG^CCA
1977 TGGCCA
NspI 523 ACATGT [GA]CATG^[CT]
808 ACATGC
PvuII 568 CAGCTG CAG^CTG
1457 CAGCTG
SacII 41 CCGCGG CCGC^GG
83 CCGCGG
SfcI 1200 CTACAG C^T[GA][CT]AG
2423 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaI AvaII BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII