##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AccI AflII AgeI AscI
AseI AsnI Asp700I Asp718I AvrII
BamHI BbrPI BbvCI BciVI BclI
BfaI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsiWI BspDI BspHI
BsrDI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EcoO65I EcoRI
EcoRV FbaI FseI HincII HindII
HpaI KpnI MaeI MamI MfeI
MluI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI PvuII RcaI
RsrII SalI SapI SbfI ScaI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I StuI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AatII 1696 GACGTC GACGT^C
AccIII 487 TCCGGA T^CCGGA
AclI 1724 AACGTT AA^CGTT
AcsI 1707 AAATTT [GA]^AATT[CT]
AflIII 283 ACATGT A^C[GA][CT]GT
AhdI 279 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1501 GTGCAC G^TGCAC
AocI 256 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2229 AGCGCT AGC^GCT
ApaLI 1501 GTGCAC G^TGCAC
ApoI 1707 AAATTT [GA]^AATT[CT]
AspEI 279 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1854 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 593 TGCGCA TGC^GCA
BbeI 132 GGCGCC GGCGC^C
BglI 2192 GCCAGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1372 AGATCT A^GATCT
BsaWI 487 TCCGGA [AT]^CCGG[AT]
BseAI 487 TCCGGA T^CCGGA
BsgI 862 GTGCAG GTGCAG(16/14)
BsiEI 14 CGGCCG CG[GA][CT]^CG
BsmBI 2056 CGTCTC CGTCTC(1/5)
BspEI 487 TCCGGA T^CCGGA
BspLU11I 283 ACATGT A^CATGT
BspMI 596 GCAGGT ACCTGC(4/8)
BstXI 2281 CCACACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 256 CCTCAGG CC^T[ACGT]AGG
DraIII 2408 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 14 CGGCCG C^GGCCG
Eam1105I 279 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 668 GAAGAG CTCTTC(1/4)
EclXI 14 CGGCCG C^GGCCG
Eco47III 2229 AGCGCT AGC^GCT
Eco52I 14 CGGCCG C^GGCCG
Eco81I 256 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1554 ATGCAT ATGCA^T
FspI 593 TGCGCA TGC^GCA
KasI 132 GGCGCC G^GCGCC
Ksp632I 668 GAAGAG CTCTTC(1/4)
MroI 487 TCCGGA T^CCGGA
NarI 132 GGCGCC GG^CGCC
NsiI 1554 ATGCAT ATGCA^T
PaeR7I 1368 CTCGAG C^TCGAG
PflFI 1854 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 920 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 570 GAGTC GAGTC(4/5)
Psp1406I 1724 AACGTT AA^CGTT
SexAI 925 ACCTGGT A^CC[AT]GGT
SfoI 132 GGCGCC GGC^GCC
SmlI 1368 CTCGAG C^T[CT][GA]AG
SphI 1556 GCATGC GCATG^C
SspI 1348 AATATT AAT^ATT
TaqI 1369 TCGA T^CGA
Tth111I 1854 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1369 TCGA T^CGA
Van91I 920 CCACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 787 CCAAAGAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1368 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
ApaI 2208 GGGCCC GGGCC^C
2374 GGGCCC
AvaII 995 GGACC G^G[AT]CC
1392 GGACC
BalI 643 TGGCCA TGG^CCA
1033 TGGCCA
BanI 132 GGCGCC G^G[CT][GA]CC
2325 GGTGCC
BcgI 106 CGAGGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
596 GCAGGTTCGTCG
BsmI 560 GAATGC GAATGC(1/-1)
1575 GAATGC
BssSI 2026 CACGAG CACGAG(-5/-1)
1498 CTCGTG
BstAPI 581 GCAGAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
656 GCAGAAACTGC
DraI 1937 TTTAAA TTT^AAA
2445 TTTAAA
EcoNI 518 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1878 CCTGACGGAGG
HindIII 324 AAGCTT A^AGCTT
379 AAGCTT
KspI 61 CCGCGG CCGC^GG
868 CCGCGG
MluNI 643 TGGCCA TGG^CCA
1033 TGGCCA
MscI 643 TGGCCA TGG^CCA
1033 TGGCCA
MspA1I 61 CCGCGG C[AC]G^C[GT]G
868 CCGCGG
NspI 283 ACATGT [GA]CATG^[CT]
1556 GCATGC
PpuMI 994 AGGACCT [GA]G^G[AT]CC[CT]
1391 GGGACCT
PstI 663 CTGCAG CTGCA^G
1717 CTGCAG
SacI 510 GAGCTC GAGCT^C
1237 GAGCTC
SacII 61 CCGCGG CCGC^GG
868 CCGCGG
SmaI 2049 CCCGGG CCC^GGG
2183 CCCGGG
XmaCI 2049 CCCGGG C^CCGGG
2183 CCCGGG
XmaI 2049 CCCGGG C^CCGGG
2183 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrBI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII