##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspEI AvrII BamHI BbvCI BciVI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsiWI Bsp1407I BspDI BspHI
BsrFI BsrGI Bst1107I BstBI BstZ17I
Bsu36I CelII Cfr10I ClaI DraI
DrdI Eam1105I Eco47III Eco81I EcoRI
EcoRV EcoT14I EcoT22I FseI HpaI
MfeI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshBI PvuI RcaI SapI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SspBI SspI StyI SwaI
TfiI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AatII 613 GACGTC GACGT^C
AccIII 1388 TCCGGA T^CCGGA
AclI 1824 AACGTT AA^CGTT
Asp700I 1658 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 775 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 698 TGCGCA TGC^GCA
BbrPI 2218 CACGTG CAC^GTG
BclI 1010 TGATCA T^GATCA
BfaI 1217 CTAG C^TAG
BsaBI 957 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1496 GGTCTC GGTCTC(1/5)
BsaWI 1388 TCCGGA [AT]^CCGG[AT]
BseAI 1388 TCCGGA T^CCGGA
BsgI 355 GTGCAG GTGCAG(16/14)
BsiEI 64 CGGCCG CG[GA][CT]^CG
BsmI 1865 GCATTC GAATGC(1/-1)
BspEI 1388 TCCGGA T^CCGGA
BspLU11I 170 ACATGT A^CATGT
BsrBI 2209 GAGCGG CCGCTC(-3/-3)
BsrDI 689 GCAATG GCAATG(2/0)
BssHII 2022 GCGCGC G^CGCGC
BssSI 2107 CACGAG CACGAG(-5/-1)
BstEII 2242 GGTCACC G^GT[ACGT]ACC
BstPI 2242 GGTCACC G^GT[ACGT]ACC
CpoI 571 CGGTCCG CG^G[AT]CCG
EagI 64 CGGCCG C^GGCCG
EclXI 64 CGGCCG C^GGCCG
Eco52I 64 CGGCCG C^GGCCG
EcoO65I 2242 GGTCACC G^GT[ACGT]ACC
FbaI 1010 TGATCA T^GATCA
FspI 698 TGCGCA TGC^GCA
HindIII 1716 AAGCTT A^AGCTT
MaeI 1217 CTAG C^TAG
MamI 957 GATATGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 314 ACGCGT A^CGCGT
MroI 1388 TCCGGA T^CCGGA
PflFI 775 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2218 CACGTG CAC^GTG
PmlI 2218 CACGTG CAC^GTG
PshAI 1565 GACACTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1824 AACGTT AA^CGTT
PvuII 1743 CAGCTG CAG^CTG
RsrII 571 CGGTCCG CG^G[AT]CCG
SalI 1562 GTCGAC G^TCGAC
SbfI 483 CCTGCAGG CCTGCA^GG
ScaI 371 AGTACT AGT^ACT
SmaI 1173 CCCGGG CCC^GGG
SphI 746 GCATGC GCATG^C
Sse8387I 483 CCTGCAGG CCTGCA^GG
Tth111I 775 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1173 CCCGGG C^CCGGG
XmaI 1173 CCCGGG C^CCGGG
XmnI 1658 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1334 GGTACC G^GTACC
1707 GGTACC
AccI 109 GTCTAC GT^[AC][GT]AC
1562 GTCGAC
AcsI 1659 AAATTT [GA]^AATT[CT]
2290 AAATTC
AlwNI 1339 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1514 CAGCCTCTG
ApoI 1659 AAATTT [GA]^AATT[CT]
2290 AAATTC
Asp718I 1334 GGTACC G^GTACC
1707 GGTACC
BalI 1502 TGGCCA TGG^CCA
1721 TGGCCA
BbeI 4 GGCGCC GGCGC^C
2081 GGCGCC
BcgI 701 GCAGCGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1793 GCAGGGGCGTCG
BpmI 385 CTGGAG CTGGAG(16/14)
826 CTGGAG
BsaAI 112 TACGTG [CT]AC^GT[GA]
2218 CACGTG
BstAPI 186 GCACTTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1865 GCATTCGTTGC
BstXI 197 CCATCCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1049 CCAACAACGTGG
DraIII 1934 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2258 CACCCTGTG
EarI 1326 GAAGAG CTCTTC(1/4)
1593 GAAGAG
EcoNI 342 CCTCTATAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1537 CCTTCCCCAGG
KasI 4 GGCGCC G^GCGCC
2081 GGCGCC
KpnI 1334 GGTACC GGTAC^C
1707 GGTACC
Ksp632I 1326 GAAGAG CTCTTC(1/4)
1593 GAAGAG
KspI 532 CCGCGG CCGC^GG
801 CCGCGG
MluNI 1502 TGGCCA TGG^CCA
1721 TGGCCA
MscI 1502 TGGCCA TGG^CCA
1721 TGGCCA
MseI 1124 TTAA T^TAA
2288 TTAA
NarI 4 GGCGCC GG^CGCC
2081 GGCGCC
NspI 170 ACATGT [GA]CATG^[CT]
746 GCATGC
PflMI 510 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1472 CCACCAGATGG
SacII 532 CCGCGG CCGC^GG
801 CCGCGG
SfoI 4 GGCGCC GGC^GCC
2081 GGCGCC
StuI 76 AGGCCT AGG^CCT
2068 AGGCCT
Tru9I 1124 TTAA T^TAA
2288 TTAA
Tsp509I 1660 AATT ^AATT
2291 AATT
Van91I 510 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1472 CCACCAGATGG
XcmI 649 CCAGGAGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2090 CCAGGGCCAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BmyI BpuAI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I TspRI TthHB8I XhoII