##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AspI BamHI BbeI
BbvCI BcgI BciVI BclI BfrI
BglI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII Hin1I HincII
HindII HindIII HpaI KasI MfeI
MluI MroI MunI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1530 GGTACC G^GTACC
AccI 741 GTAGAC GT^[AC][GT]AC
AflIII 1813 ACATGT A^C[GA][CT]GT
Asp700I 1695 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1530 GGTACC G^GTACC
AvaI 90 CTCGAG C^[CT]CG[GA]G
AviII 323 TGCGCA TGC^GCA
AvrII 96 CCTAGG C^CTAGG
BanI 1530 GGTACC G^G[CT][GA]CC
BbsI 181 GAAGAC GAAGAC(2/6)
BlnI 96 CCTAGG C^CTAGG
BlpI 382 GCTGAGC GC^T[ACGT]AGC
BpmI 1593 CTGGAG CTGGAG(16/14)
Bpu1102I 382 GCTGAGC GC^T[ACGT]AGC
BpuAI 181 GAAGAC GAAGAC(2/6)
BsaBI 1223 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 118 ACCGGA [AT]^CCGG[AT]
BsgI 960 CTGCAC GTGCAG(16/14)
BsoBI 90 CTCGAG C^[CT]CG[GA]G
BspLU11I 1813 ACATGT A^CATGT
BsrBI 1058 CCGCTC CCGCTC(-3/-3)
BsrDI 1841 CATTGC GCAATG(2/0)
BsrFI 451 ACCGGC [GA]^CCGG[CT]
BstXI 699 CCATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 382 GCTGAGC GC^T[ACGT]AGC
CfoI 324 GCGC GCG^C
Cfr10I 451 ACCGGC [GA]^CCGG[CT]
DraI 1753 TTTAAA TTT^AAA
DraIII 1338 CACGTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1567 CCGCGG C^C[GA][CT]GG
FspI 323 TGCGCA TGC^GCA
HhaI 324 GCGC GCG^C
HinP1I 324 GCGC G^CGC
KpnI 1530 GGTACC GGTAC^C
KspI 1567 CCGCGG CCGC^GG
MamI 1223 GATGACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 1567 CCGCGG C[AC]G^C[GT]G
NdeI 1317 CATATG CA^TATG
PaeR7I 90 CTCGAG C^TCGAG
SacI 1005 GAGCTC GAGCT^C
SacII 1567 CCGCGG CCGC^GG
XcmI 1927 CCACTTCCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 90 CTCGAG C^TCGAG
XmnI 1695 GAAACTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
ApaI 68 GGGCCC GGGCC^C
1361 GGGCCC
BalI 2199 TGGCCA TGG^CCA
2317 TGGCCA
BcnI 31 CCCGG CC^[GC]GG
66 CCGGG
BglII 956 AGATCT A^GATCT
1973 AGATCT
BsmAI 666 GAGAC GTCTC(1/5)
1437 GAGAC
BstYI 956 AGATCT [GA]^GATC[CT]
1973 AGATCT
EaeI 2199 TGGCCA [CT]^GGCC[GA]
2317 TGGCCA
HgaI 647 GACGC GACGC(5/10)
47 GCGTC
MflI 956 AGATCT [GA]^GATC[CT]
1973 AGATCT
MluNI 2199 TGGCCA TGG^CCA
2317 TGGCCA
MscI 2199 TGGCCA TGG^CCA
2317 TGGCCA
NciI 31 CCCGG CC^[GC]GG
66 CCGGG
NheI 334 GCTAGC G^CTAGC
2038 GCTAGC
NspI 126 GCATGT [GA]CATG^[CT]
1813 ACATGT
SapI 1002 GAAGAGC GCTCTTC(1/4)
493 GCTCTTC
ScaI 574 AGTACT AGT^ACT
1884 AGTACT
SpeI 973 ACTAGT A^CTAGT
1887 ACTAGT
XhoII 956 AGATCT [GA]^GATC[CT]
1973 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI AvaII BanII BbrPI
BbvI BfaI BmyI BsaAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BssSI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PflMI PleI PmaCI
PmlI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I