##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbvCI BciVI
BclI BfrI BlnI BsaAI BsaWI
BseAI BseRI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BspMI Bst1107I
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraIII DrdI
Eam1105I EarI Eco81I EcoO65I EcoRI
EcoRV FbaI FokI FseI FspI
HpaI KpnI Ksp632I MaeIII MfeI
MluI MroI MunI NcoI NheI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspI StuI
SwaI Tsp45I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AcsI 1818 AAATTT [GA]^AATT[CT]
AcyI 96 GGCGCC G[GA]^CG[CT]C
AlwI 1602 GGATC GGATC(4/5)
Aor51HI 118 AGCGCT AGC^GCT
ApoI 1818 AAATTT [GA]^AATT[CT]
AseI 1361 ATTAAT AT^TAAT
AsnI 1361 ATTAAT AT^TAAT
BbeI 96 GGCGCC GGCGC^C
BcgI 1466 GCATGAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1287 CTAG C^TAG
BglII 1136 AGATCT A^GATCT
BlpI 155 GCTGAGC GC^T[ACGT]AGC
BpmI 1339 CTCCAG CTGGAG(16/14)
Bpu1102I 155 GCTGAGC GC^T[ACGT]AGC
BsaBI 1358 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 96 GGCGCC G[GA]^CG[CT]C
BsaI 88 GAGACC GGTCTC(1/5)
BsmAI 88 GAGAC GTCTC(1/5)
BsrBI 427 GAGCGG CCGCTC(-3/-3)
BsrDI 860 GCAATG GCAATG(2/0)
BssHII 483 GCGCGC G^CGCGC
BssSI 424 CACGAG CACGAG(-5/-1)
BstAPI 1494 GCACAAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1474 TTCGAA TT^CGAA
BstYI 1136 AGATCT [GA]^GATC[CT]
CelII 155 GCTGAGC GC^T[ACGT]AGC
EagI 78 CGGCCG C^GGCCG
EclXI 78 CGGCCG C^GGCCG
Eco47III 118 AGCGCT AGC^GCT
Eco52I 78 CGGCCG C^GGCCG
EcoNI 647 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 918 CCTTGG C^C[AT][AT]GG
EcoT22I 1528 ATGCAT ATGCA^T
HgaI 170 GACGC GACGC(5/10)
Hin1I 96 GGCGCC G[GA]^CG[CT]C
HincII 1038 GTTGAC GT[CT]^[GA]AC
HindII 1038 GTTGAC GT[CT]^[GA]AC
HindIII 1806 AAGCTT A^AGCTT
KasI 96 GGCGCC G^GCGCC
KspI 495 CCGCGG CCGC^GG
MaeI 1287 CTAG C^TAG
MaeII 849 ACGT A^CGT
MamI 1358 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1136 AGATCT [GA]^GATC[CT]
MslI 310 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 559 GCCGGC GCC^GGC
NarI 96 GGCGCC GG^CGCC
NgoMIV 559 GCCGGC G^CCGGC
NotI 77 GCGGCCGC GC^GGCCGC
NruI 106 TCGCGA TCG^CGA
NsiI 1528 ATGCAT ATGCA^T
NspV 1474 TTCGAA TT^CGAA
PleI 1041 GACTC GAGTC(4/5)
PshBI 1361 ATTAAT AT^TAAT
PvuI 1045 CGATCG CGAT^CG
SacII 495 CCGCGG CCGC^GG
SfiI 490 GGCCCCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 96 GGCGCC GGC^GCC
SfuI 1474 TTCGAA TT^CGAA
StyI 918 CCTTGG C^C[AT][AT]GG
TfiI 65 GAATC G^A[AT]TC
XhoII 1136 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspHI 389 GAGCAC G[AT]GC[AT]^C
1344 GTGCTC
BalI 246 TGGCCA TGG^CCA
537 TGGCCA
BanII 178 GAGCCC G[GA]GC[CT]^C
331 GAGCCC
BbsI 191 GTCTTC GAAGAC(2/6)
1739 GTCTTC
BpuAI 191 GTCTTC GAAGAC(2/6)
1739 GTCTTC
BsgI 287 CTGCAC GTGCAG(16/14)
1492 CTGCAC
BsiEI 78 CGGCCG CG[GA][CT]^CG
1045 CGATCG
BsiHKAI 389 GAGCAC G[AT]GC[AT]^C
1344 GTGCTC
BsmI 418 GAATGC GAATGC(1/-1)
1422 GCATTC
BsrFI 160 GCCGGT [GA]^CCGG[CT]
559 GCCGGC
Cfr10I 160 GCCGGT [GA]^CCGG[CT]
559 GCCGGC
DdeI 156 CTGAG C^T[ACGT]AG
1112 CTTAG
DraI 1196 TTTAAA TTT^AAA
1551 TTTAAA
DraII 137 AGGTCCC [GA]G^G[ACGT]CC[CT]
526 GGGTCCC
EcoO109I 137 AGGTCCC [GA]G^G[ACGT]CC[CT]
526 GGGTCCC
HinfI 65 GAATC G^A[ACGT]TC
1041 GACTC
MluNI 246 TGGCCA TGG^CCA
537 TGGCCA
MscI 246 TGGCCA TGG^CCA
537 TGGCCA
NdeI 239 CATATG CA^TATG
1666 CATATG
PpuMI 137 AGGTCCC [GA]G^G[AT]CC[CT]
526 GGGTCCC
PstI 575 CTGCAG CTGCA^G
651 CTGCAG
PvuII 1 CAGCTG CAG^CTG
572 CAGCTG
SfaNI 432 GCATC GCATC(5/9)
945 GCATC
SfcI 575 CTGCAG C^T[GA][CT]AG
651 CTGCAG
SphI 439 GCATGC GCATG^C
567 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanI BbvI BcnI BglI
BmyI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DpnII DsaI EaeI EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
RsaI Sau3AI Sau96I ScrFI SspBI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I Van91I