##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BlnI BlpI Bpu1102I
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmFI BsmI
BsoBI BspDI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstPI BstXI Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI Hin1I
HindIII HpaII KasI KpnI KspI
MluI MroI MspA1I MspI MwoI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SpeI
SphI Sse8387I StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AflIII 1063 ACATGT A^C[GA][CT]GT
Asp700I 962 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 128 GGACC G^G[AT]CC
BalI 1255 TGGCCA TGG^CCA
BanII 922 GGGCTC G[GA]GC[CT]^C
BbsI 561 GAAGAC GAAGAC(2/6)
BbvCI 935 GCTGAGG CCTCAGC(-5/-2)
BglII 827 AGATCT A^GATCT
BmyI 922 GGGCTC G[AGT]GC[ACT]^C
BpmI 1090 CTCCAG CTGGAG(16/14)
BpuAI 561 GAAGAC GAAGAC(2/6)
BsaAI 1439 TACGTA [CT]AC^GT[GA]
BsaBI 45 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 1247 CCAGATGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1247 CCAGATGTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 1011 CGTCTC CGTCTC(1/5)
Bsp1286I 922 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 1501 TGTACA T^GTACA
BspLU11I 1063 ACATGT A^CATGT
BsrGI 1501 TGTACA T^GTACA
Bst1107I 171 GTATAC GTA^TAC
BstBI 802 TTCGAA TT^CGAA
BstZ17I 171 GTATAC GTA^TAC
CfoI 33 GCGC GCG^C
Cfr13I 128 GGACC G^G[ACGT]CC
DraI 1479 TTTAAA TTT^AAA
DsaI 556 CCATGG C^C[GA][CT]GG
EaeI 1255 TGGCCA [CT]^GGCC[GA]
EcoT14I 556 CCATGG C^C[AT][AT]GG
HhaI 33 GCGC GCG^C
HinP1I 33 GCGC G^CGC
HpaI 903 GTTAAC GTT^AAC
MamI 45 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 271 CAATTG C^AATTG
MluNI 1255 TGGCCA TGG^CCA
MscI 1255 TGGCCA TGG^CCA
MslI 1058 CATTAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 271 CAATTG C^AATTG
NcoI 556 CCATGG C^CATGG
NruI 686 TCGCGA TCG^CGA
NspI 1063 ACATGT [GA]CATG^[CT]
NspV 802 TTCGAA TT^CGAA
PflMI 1247 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1485 GAAGAGC GCTCTTC(1/4)
Sau96I 128 GGACC G^G[ACGT]CC
SfuI 802 TTCGAA TT^CGAA
SnaBI 1439 TACGTA TAC^GTA
SspBI 1501 TGTACA T^GTACA
StyI 556 CCATGG C^C[AT][AT]GG
TaqI 803 TCGA T^CGA
Tsp45I 1412 GTGAC ^GT[GC]AC
TthHB8I 803 TCGA T^CGA
Van91I 1247 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 413 CCAGTACGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 962 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 153 GTAGAC GT^[AC][GT]AC
171 GTATAC
AccII 32 CGCG CG^CG
687 CGCG
AhdI 743 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1028 GACTTTCTGTC
AlwI 291 GATCC GGATC(4/5)
44 GGATC
AspEI 743 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1028 GACTTTCTGTC
BbvI 441 GCAGC GCAGC(8/12)
552 GCAGC
BfaI 54 CTAG C^TAG
831 CTAG
BsaI 582 GAGACC GGTCTC(1/5)
652 GGTCTC
BsaJI 322 CCAGGG C^C[ACGT][ACGT]GG
556 CCATGG
BspMI 815 GCAGGT ACCTGC(4/8)
1291 GCAGGT
BssKI 322 CCAGG ^CC[ACGT]GG
350 CCTGG
BstNI 322 CCAGG CC^[AT]GG
350 CCTGG
BstUI 32 CGCG CG^CG
687 CGCG
BstYI 290 AGATCC [GA]^GATC[CT]
827 AGATCT
Cac8I 518 GCTGGC GC[ACGT]^[ACGT]GC
1490 GCCTGC
Eam1105I 743 GACAATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1028 GACTTTCTGTC
EcoRII 322 CCAGG ^CC[AT]GG
350 CCTGG
Fnu4HI 441 GCAGC GC^[ACGT]GC
552 GCAGC
HaeIII 521 GGCC GG^CC
1256 GGCC
HincII 317 GTCAAC GT[CT]^[GA]AC
903 GTTAAC
HindII 317 GTCAAC GT[CT]^[GA]AC
903 GTTAAC
ItaI 441 GCAGC GC^[ACGT]GC
552 GCAGC
MaeI 54 CTAG C^TAG
831 CTAG
MaeII 809 ACGT A^CGT
1440 ACGT
MaeIII 1183 GTAAC ^GT[ACGT]AC
1412 GTGAC
MflI 290 AGATCC [GA]^GATC[CT]
827 AGATCT
MvaI 322 CCAGG CC^[AT]GG
350 CCTGG
MvnI 32 CGCG CG^CG
687 CGCG
NlaIV 473 GGTTCC GG[ACGT]^[ACGT]CC
1301 GGCTCC
PleI 1088 GACTC GAGTC(4/5)
852 GAGTC
ScrFI 322 CCAGG CC^[ACGT]GG
350 CCTGG
SfcI 395 CTACAG C^T[GA][CT]AG
775 CTACAG
SmlI 987 CTTGAG C^T[CT][GA]AG
1318 CTCAAG
TseI 441 GCAGC G^C[AT]GC
552 GCAGC
XhoII 290 AGATCC [GA]^GATC[CT]
827 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BsmAI BsrI BstF5I DdeI DpnII
EarI FokI HinfI HphI Ksp632I
MboI MboII MnlI MseI NdeII
NlaIII RsaI Sau3AI SfaNI SspI
TfiI Tru9I Tsp509I TspRI