##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BanI BbeI BbvCI BcgI BcnI
BfrI BglI BglII BsaHI BsaI
BsaWI BseAI BsiEI BsmBI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I CfoI Cfr10I
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FauI FseI FspI HaeII HapII
HhaI Hin1I HinP1I HpaI HpaII
KasI KpnI KspI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AocI 1510 CCTGAGG CC^T[ACGT]AGG
Asp700I 2214 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 159 GAGCTC G[AT]GC[AT]^C
AvaI 465 CTCGGG C^[CT]CG[GA]G
AvrII 1348 CCTAGG C^CTAGG
BalI 452 TGGCCA TGG^CCA
BamHI 893 GGATCC G^GATCC
BanII 159 GAGCTC G[GA]GC[CT]^C
BbrPI 1554 CACGTG CAC^GTG
BciVI 874 GTATCC GTATCC(6/5)
BclI 1785 TGATCA T^GATCA
BlnI 1348 CCTAGG C^CTAGG
BpmI 2147 CTCCAG CTGGAG(16/14)
BsiHKAI 159 GAGCTC G[AT]GC[AT]^C
BsiWI 85 CGTACG C^GTACG
BsmI 370 GAATGC GAATGC(1/-1)
BsoBI 465 CTCGGG C^[CT]CG[GA]G
Bsp1407I 587 TGTACA T^GTACA
BspDI 1691 ATCGAT AT^CGAT
BspLU11I 275 ACATGT A^CATGT
BsrDI 45 CATTGC GCAATG(2/0)
BsrGI 587 TGTACA T^GTACA
Bsu36I 1510 CCTGAGG CC^T[ACGT]AGG
ClaI 1691 ATCGAT AT^CGAT
DraI 1635 TTTAAA TTT^AAA
DraII 1121 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 81 GACACGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2018 CCATGG C^C[GA][CT]GG
EaeI 452 TGGCCA [CT]^GGCC[GA]
Eco81I 1510 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1121 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 980 GAATTC G^AATTC
EcoT22I 2059 ATGCAT ATGCA^T
FbaI 1785 TGATCA T^GATCA
HincII 751 GTTGAC GT[CT]^[GA]AC
HindII 751 GTTGAC GT[CT]^[GA]AC
MluNI 452 TGGCCA TGG^CCA
MscI 452 TGGCCA TGG^CCA
MspA1I 869 CAGCTG C[AC]G^C[GT]G
NcoI 2018 CCATGG C^CATGG
NdeI 542 CATATG CA^TATG
NsiI 2059 ATGCAT ATGCA^T
PflMI 1155 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1554 CACGTG CAC^GTG
PmlI 1554 CACGTG CAC^GTG
PvuII 869 CAGCTG CAG^CTG
SacI 159 GAGCTC GAGCT^C
SmlI 908 CTCAAG C^T[CT][GA]AG
SphI 206 GCATGC GCATG^C
SspBI 587 TGTACA T^GTACA
Van91I 1155 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 402 CCACAACCCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2214 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 1003 GGACC G^G[AT]CC
1714 GGACC
BlpI 1891 GCTGAGC GC^T[ACGT]AGC
2083 GCTAAGC
BmyI 159 GAGCTC G[AGT]GC[ACT]^C
614 GGGCAC
Bpu1102I 1891 GCTGAGC GC^T[ACGT]AGC
2083 GCTAAGC
BsaAI 83 CACGTA [CT]AC^GT[GA]
1554 CACGTG
BsiYI 1155 CCATGTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1647 CCCGAATGAGG
BslI 1155 CCATGTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1647 CCCGAATGAGG
Bsp1286I 159 GAGCTC G[AGT]GC[ACT]^C
614 GGGCAC
BspMI 1129 GCAGGT ACCTGC(4/8)
1135 GCAGGT
CelII 1891 GCTGAGC GC^T[ACGT]AGC
2083 GCTAAGC
EarI 917 CTCTTC CTCTTC(1/4)
1843 GAAGAG
HgaI 1915 GACGC GACGC(5/10)
1204 GCGTC
HindIII 112 AAGCTT A^AGCTT
1181 AAGCTT
Ksp632I 917 CTCTTC CTCTTC(1/4)
1843 GAAGAG
NspI 206 GCATGC [GA]CATG^[CT]
275 ACATGT
PstI 24 CTGCAG CTGCA^G
988 CTGCAG
StuI 687 AGGCCT AGG^CCT
1878 AGGCCT
TspRI 1194 CACTG CA[GC]TG[ACGT][ACGT]^
1412 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI BbsI
BbvI BfaI BpuAI BsaBI BsaJI
BseRI BsgI BsmAI BsmFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MamI
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TthHB8I XhoII