##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbrPI BbvCI BcgI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI Eam1105I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI HincII HindII HpaI KpnI
KspI MamI MfeI MflI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tth111I TthHB8I XbaI
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 1588 GAATTT [GA]^AATT[CT]
Aor51HI 81 AGCGCT AGC^GCT
ApoI 1588 GAATTT [GA]^AATT[CT]
AviII 1856 TGCGCA TGC^GCA
BbeI 975 GGCGCC GGCGC^C
BglI 298 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1670 TACGTG [CT]AC^GT[GA]
BsmI 1030 GAATGC GAATGC(1/-1)
BspMI 1784 GCAGGT ACCTGC(4/8)
BsrBI 252 GAGCGG CCGCTC(-3/-3)
BssSI 996 CTCGTG CACGAG(-5/-1)
BstAPI 850 GCATGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 491 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 387 GACCGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 81 AGCGCT AGC^GCT
FspI 1856 TGCGCA TGC^GCA
HgaI 646 GCGTC GACGC(5/10)
HindIII 1287 AAGCTT A^AGCTT
KasI 975 GGCGCC G^GCGCC
NarI 975 GGCGCC GG^CGCC
NotI 416 GCGGCCGC GC^GGCCGC
NspI 464 GCATGT [GA]CATG^[CT]
PpuMI 379 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 1716 CAGCTG CAG^CTG
SexAI 1501 ACCTGGT A^CC[AT]GGT
SfoI 975 GGCGCC GGC^GCC
SmaI 411 CCCGGG CCC^GGG
SmlI 780 CTCAAG C^T[CT][GA]AG
TfiI 153 GATTC G^A[AT]TC
Tsp509I 1589 AATT ^AATT
XmaCI 411 CCCGGG C^CCGGG
XmaI 411 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 21 GACGTC GACGT^C
257 GACGTC
AccII 289 CGCG CG^CG
959 CGCG
AflIII 67 ACGTGT A^C[GA][CT]GT
1671 ACGTGT
ApaI 314 GGGCCC GGGCC^C
872 GGGCCC
BalI 796 TGGCCA TGG^CCA
1384 TGGCCA
BbsI 1179 GAAGAC GAAGAC(2/6)
728 GTCTTC
BfaI 927 CTAG C^TAG
1624 CTAG
BpuAI 1179 GAAGAC GAAGAC(2/6)
728 GTCTTC
BspHI 637 TCATGA T^CATGA
904 TCATGA
BstUI 289 CGCG CG^CG
959 CGCG
BstXI 664 CCATCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1701 CCAGCTCTTTGG
MaeI 927 CTAG C^TAG
1624 CTAG
MluNI 796 TGGCCA TGG^CCA
1384 TGGCCA
MscI 796 TGGCCA TGG^CCA
1384 TGGCCA
MvnI 289 CGCG CG^CG
959 CGCG
PstI 885 CTGCAG CTGCA^G
1581 CTGCAG
RcaI 637 TCATGA T^CATGA
904 TCATGA
SapI 435 GAAGAGC GCTCTTC(1/4)
1829 GAAGAGC
StuI 425 AGGCCT AGG^CCT
892 AGGCCT
XcmI 664 CCATCTTCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1492 CCACGGACAACCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaI AvaII BanI BanII BbvI
BclI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI Tru9I TseI
Tsp45I TspRI Van91I