##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BciVI BclI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiWI BsmI BspDI BspEI BspMI
BsrBI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HgaI KpnI MamI MfeI MluI
MroI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmlI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 583 GTAGAC GT^[AC][GT]AC
AcyI 203 GGCGCC G[GA]^CG[CT]C
AflII 1309 CTTAAG C^TTAAG
AflIII 1480 ACATGT A^C[GA][CT]GT
Aor51HI 809 AGCGCT AGC^GCT
ApaI 278 GGGCCC GGGCC^C
AspHI 1628 GAGCAC G[AT]GC[AT]^C
AvaI 1121 CTCGGG C^[CT]CG[GA]G
BalI 985 TGGCCA TGG^CCA
BanII 278 GGGCCC G[GA]GC[CT]^C
BbeI 203 GGCGCC GGCGC^C
BcgI 248 GCAACGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1309 CTTAAG C^TTAAG
BsaHI 203 GGCGCC G[GA]^CG[CT]C
BsaI 653 GGTCTC GGTCTC(1/5)
BsgI 435 CTGCAC GTGCAG(16/14)
BsiEI 337 CGGCCG CG[GA][CT]^CG
BsiHKAI 1628 GAGCAC G[AT]GC[AT]^C
BsmBI 482 CGTCTC CGTCTC(1/5)
BsoBI 1121 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1763 TGTACA T^GTACA
BspHI 1658 TCATGA T^CATGA
BspLU11I 1480 ACATGT A^CATGT
BsrFI 236 ACCGGC [GA]^CCGG[CT]
BsrGI 1763 TGTACA T^GTACA
Cfr10I 236 ACCGGC [GA]^CCGG[CT]
DraI 1576 TTTAAA TTT^AAA
EagI 337 CGGCCG C^GGCCG
EclXI 337 CGGCCG C^GGCCG
Eco47III 809 AGCGCT AGC^GCT
Eco52I 337 CGGCCG C^GGCCG
EcoRI 1039 GAATTC G^AATTC
EcoT14I 1506 CCTTGG C^C[AT][AT]GG
Hin1I 203 GGCGCC G[GA]^CG[CT]C
HincII 675 GTTAAC GT[CT]^[GA]AC
HindII 675 GTTAAC GT[CT]^[GA]AC
HindIII 530 AAGCTT A^AGCTT
HpaI 675 GTTAAC GTT^AAC
HphI 1335 GGTGA GGTGA(8/7)
KasI 203 GGCGCC G^GCGCC
MaeIII 932 GTGAC ^GT[ACGT]AC
MluNI 985 TGGCCA TGG^CCA
MscI 985 TGGCCA TGG^CCA
MslI 1243 CACTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 203 GGCGCC GG^CGCC
NspI 1480 ACATGT [GA]CATG^[CT]
PmeI 1575 GTTTAAAC GTTT^AAAC
PshAI 391 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 122 CTGCAG CTGCA^G
RcaI 1658 TCATGA T^CATGA
SfaNI 979 GCATC GCATC(5/9)
SfcI 122 CTGCAG C^T[GA][CT]AG
SfoI 203 GGCGCC GGC^GCC
SgrAI 235 CACCGGCG C[GA]^CCGG[CT]G
SspBI 1763 TGTACA T^GTACA
StuI 345 AGGCCT AGG^CCT
StyI 1506 CCTTGG C^C[AT][AT]GG
Tsp45I 932 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
BanI 203 GGCGCC G^G[CT][GA]CC
259 GGTGCC
BbsI 1114 GAAGAC GAAGAC(2/6)
133 GTCTTC
BmyI 278 GGGCCC G[AGT]GC[ACT]^C
1628 GAGCAC
BpuAI 1114 GAAGAC GAAGAC(2/6)
133 GTCTTC
BsmAI 483 GTCTC GTCTC(1/5)
654 GTCTC
Bsp1286I 278 GGGCCC G[AGT]GC[ACT]^C
1628 GAGCAC
BsrDI 1053 CATTGC GCAATG(2/0)
938 GCAATG
BstNI 214 CCAGG CC^[AT]GG
404 CCAGG
BstYI 126 AGATCC [GA]^GATC[CT]
1425 AGATCC
CfoI 204 GCGC GCG^C
810 GCGC
DraII 27 GGGACCC [GA]G^G[ACGT]CC[CT]
377 GGGACCC
EarI 1378 CTCTTC CTCTTC(1/4)
624 GAAGAG
EcoO109I 27 GGGACCC [GA]G^G[ACGT]CC[CT]
377 GGGACCC
EcoRII 214 CCAGG ^CC[AT]GG
404 CCAGG
HaeII 203 GGCGCC [GA]GCGC^[CT]
809 AGCGCT
HhaI 204 GCGC GCG^C
810 GCGC
HinP1I 204 GCGC G^CGC
810 GCGC
HinfI 517 GATTC G^A[ACGT]TC
1740 GAATC
Ksp632I 1378 CTCTTC CTCTTC(1/4)
624 GAAGAG
MflI 126 AGATCC [GA]^GATC[CT]
1425 AGATCC
MvaI 214 CCAGG CC^[AT]GG
404 CCAGG
PpuMI 27 GGGACCC [GA]G^G[AT]CC[CT]
377 GGGACCC
PvuII 416 CAGCTG CAG^CTG
1390 CAGCTG
TfiI 517 GATTC G^A[AT]TC
1740 GAATC
XhoII 126 AGATCC [GA]^GATC[CT]
1425 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BbvCI
BbvI BcnI BfaI BpmI BsaJI
BsaWI BseRI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstUI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
FauI Fnu4HI FokI HaeIII HapII
HpaII ItaI KspI MaeI MaeII
MboI MboII MnlI MseI MspA1I
MspI MvnI MwoI NciI NdeII
NlaIII NlaIV RsaI SacII Sau3AI
Sau96I ScrFI SmlI SspI Tru9I
TseI Tsp509I TspRI