##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmFI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I CelII Cfr10I
ClaI CpoI DraIII EaeI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HaeII Hin1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI StuI Tth111I
Van91I XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AhdI 1568 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 480 GATCC GGATC(4/5)
AocI 1009 CCTAAGG CC^T[ACGT]AGG
AspEI 1568 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 498 GGTGCC G^G[CT][GA]CC
BciVI 1062 GTATCC GTATCC(6/5)
BcnI 64 CCCGG CC^[GC]GG
BsaAI 1032 TACGTG [CT]AC^GT[GA]
BsaJI 910 CCATGG C^C[ACGT][ACGT]GG
BsiYI 58 CCGCGACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 58 CCGCGACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 44 GAGACG CGTCTC(1/5)
BsrDI 136 CATTGC GCAATG(2/0)
BstNI 1694 CCAGG CC^[AT]GG
BstXI 832 CCATAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1009 CCTAAGG CC^T[ACGT]AGG
CfoI 31 GCGC GCG^C
DraII 1566 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 1485 GACTAAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 910 CCATGG C^C[GA][CT]GG
Eam1105I 1568 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1009 CCTAAGG CC^T[ACGT]AGG
EcoO109I 1566 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 1694 CCAGG ^CC[AT]GG
EcoT14I 910 CCATGG C^C[AT][AT]GG
EcoT22I 1073 ATGCAT ATGCA^T
HgaI 46 GACGC GACGC(5/10)
HhaI 31 GCGC GCG^C
HinP1I 31 GCGC G^CGC
HindIII 865 AAGCTT A^AGCTT
MvaI 1694 CCAGG CC^[AT]GG
NciI 64 CCCGG CC^[GC]GG
NcoI 910 CCATGG C^CATGG
NdeI 1076 CATATG CA^TATG
NsiI 1073 ATGCAT ATGCA^T
NspI 1621 GCATGT [GA]CATG^[CT]
PpuMI 1566 AGGACCT [GA]G^G[AT]CC[CT]
PstI 50 CTGCAG CTGCA^G
SapI 94 GCTCTTC GCTCTTC(1/4)
ScaI 218 AGTACT AGT^ACT
SpeI 736 ACTAGT A^CTAGT
SspI 253 AATATT AAT^ATT
StyI 910 CCATGG C^C[AT][AT]GG
SwaI 1253 ATTTAAAT ATTT^AAAT
TaqI 227 TCGA T^CGA
Tsp45I 742 GTGAC ^GT[GC]AC
TthHB8I 227 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 32 CGCG CG^CG
59 CGCG
AfaI 219 GTAC GT^AC
379 GTAC
AflIII 230 ACGTGT A^C[GA][CT]GT
1033 ACGTGT
AlwNI 190 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
280 CAGATGCTG
Asp700I 336 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1197 GAAAATTTTC
AvaII 1014 GGACC G^G[AT]CC
1567 GGACC
BsmAI 44 GAGAC GTCTC(1/5)
626 GAGAC
BsmI 638 GCATTC GAATGC(1/-1)
1103 GCATTC
BssKI 64 CCCGG ^CC[ACGT]GG
1694 CCAGG
BstUI 32 CGCG CG^CG
59 CGCG
DpnII 225 GATC ^GATC
480 GATC
FauI 57 CCCGC CCCGC(4/6)
1440 GCGGG
HapII 41 CCGG C^CGG
65 CCGG
HpaII 41 CCGG C^CGG
65 CCGG
MaeII 230 ACGT A^CGT
1033 ACGT
MboI 225 GATC ^GATC
480 GATC
MspI 41 CCGG C^CGG
65 CCGG
MvnI 32 CGCG CG^CG
59 CGCG
NdeII 225 GATC ^GATC
480 GATC
NlaIV 299 GGAACC GG[ACGT]^[ACGT]CC
498 GGTGCC
RsaI 219 GTAC GT^AC
379 GTAC
Sau3AI 225 GATC ^GATC
480 GATC
ScrFI 64 CCCGG CC^[ACGT]GG
1694 CCAGG
SmlI 19 CTTGAG C^T[CT][GA]AG
1664 CTTGAG
XbaI 1021 TCTAGA T^CTAGA
1240 TCTAGA
XmnI 336 GAAGCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1197 GAAAATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BfaI BspHI BspMI BsrI BstF5I
Cac8I Cfr13I DdeI DraI EarI
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboII
MnlI MseI MslI MwoI NlaIII
RcaI Sau96I SfaNI SfcI TfiI
Tru9I TseI Tsp509I TspRI