##################################
# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BclI
BglI BglII BlnI BpuAI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Cfr10I CpoI DraIII
DrdI EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 1495 GTAGAC GT^[AC][GT]AC
AcyI 1415 GGCGTC G[GA]^CG[CT]C
AflII 15 CTTAAG C^TTAAG
AflIII 1545 ACGTGT A^C[GA][CT]GT
AhdI 1427 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1532 AGCGCT AGC^GCT
AspEI 1427 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 496 CCTCAGC CCTCAGC(-5/-2)
BciVI 622 GGATAC GTATCC(6/5)
BfaI 743 CTAG C^TAG
BfrI 15 CTTAAG C^TTAAG
BlpI 1277 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1277 GCTCAGC GC^T[ACGT]AGC
BsaAI 1544 TACGTG [CT]AC^GT[GA]
BsaBI 514 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1415 GGCGTC G[GA]^CG[CT]C
BsaI 137 GAGACC GGTCTC(1/5)
BsmBI 1434 CGTCTC CGTCTC(1/5)
BspDI 933 ATCGAT AT^CGAT
BspMI 80 GCAGGT ACCTGC(4/8)
BstYI 1028 GGATCT [GA]^GATC[CT]
CelII 1277 GCTCAGC GC^T[ACGT]AGC
ClaI 933 ATCGAT AT^CGAT
EaeI 114 CGGCCG [CT]^GGCC[GA]
EagI 114 CGGCCG C^GGCCG
Eam1105I 1427 GACTTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1232 GAAGAG CTCTTC(1/4)
EclXI 114 CGGCCG C^GGCCG
Eco47III 1532 AGCGCT AGC^GCT
Eco52I 114 CGGCCG C^GGCCG
EcoRI 761 GAATTC G^AATTC
HaeII 1532 AGCGCT [GA]GCGC^[CT]
Hin1I 1415 GGCGTC G[GA]^CG[CT]C
HphI 918 TCACC GGTGA(8/7)
Ksp632I 1232 GAAGAG CTCTTC(1/4)
KspI 1420 CCGCGG CCGC^GG
MaeI 743 CTAG C^TAG
MamI 514 GATACAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII 1232 GAAGA GAAGA(8/7)
MflI 1028 GGATCT [GA]^GATC[CT]
NspI 201 GCATGT [GA]CATG^[CT]
PleI 1270 GACTC GAGTC(4/5)
PstI 714 CTGCAG CTGCA^G
PvuI 629 CGATCG CGAT^CG
SacII 1420 CCGCGG CCGC^GG
SapI 1232 GAAGAGC GCTCTTC(1/4)
SmlI 15 CTTAAG C^T[CT][GA]AG
SspI 1556 AATATT AAT^ATT
Tsp45I 438 GTGAC ^GT[GC]AC
XhoII 1028 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 1317 GTAC GT^AC
1543 GTAC
AlwNI 1045 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1398 CAGCAGCTG
AocI 174 CCTCAGG CC^T[ACGT]AGG
962 CCTCAGG
BanI 1010 GGCACC G^G[CT][GA]CC
1512 GGCACC
BseRI 93 CTCCTC GAGGAG(10/8)
960 CTCCTC
BsiEI 114 CGGCCG CG[GA][CT]^CG
629 CGATCG
BstF5I 550 CATCC GGATG(2/0)
998 CATCC
BstXI 696 CCATGGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1353 CCATGGACATGG
Bsu36I 174 CCTCAGG CC^T[ACGT]AGG
962 CCTCAGG
Eco81I 174 CCTCAGG CC^T[ACGT]AGG
962 CCTCAGG
EcoT14I 696 CCATGG C^C[AT][AT]GG
1353 CCATGG
FauI 902 CCCGC CCCGC(4/6)
1509 GCGGG
FokI 550 CATCC GGATG(9/13)
998 CATCC
HgaI 1416 GCGTC GACGC(5/10)
1568 GCGTC
NcoI 696 CCATGG C^CATGG
1353 CCATGG
PflMI 483 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
549 CCATCCATTGG
PpuMI 492 GGGACCT [GA]G^G[AT]CC[CT]
1327 GGGTCCC
PvuII 577 CAGCTG CAG^CTG
1401 CAGCTG
RsaI 1317 GTAC GT^AC
1543 GTAC
StyI 696 CCATGG C^C[AT][AT]GG
1353 CCATGG
Van91I 483 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
549 CCATCCATTGG
XcmI 696 CCATGGCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
997 CCATCCTGTTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EcoO109I EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MaeIII MboI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I