##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AocI Aor51HI ApaI AscI AspI
AviII AvrII BanII BbeI BbrPI
BbvCI BcgI BfrI BlnI BsaAI
BsiEI BsiWI BsmBI BspDI BspLU11I
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI FspI KasI KspI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SacII SalI SapI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
StuI SwaI Tsp45I Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 137 GGTACC G^GTACC
AccIII 849 TCCGGA T^CCGGA
AcyI 930 GACGCC G[GA]^CG[CT]C
AflIII 2165 ACGTGT A^C[GA][CT]GT
AseI 2293 ATTAAT AT^TAAT
AsnI 2293 ATTAAT AT^TAAT
Asp718I 137 GGTACC G^GTACC
AvaI 1006 CCCGGG C^[CT]CG[GA]G
BamHI 922 GGATCC G^GATCC
BciVI 2415 GTATCC GTATCC(6/5)
BclI 2394 TGATCA T^GATCA
BglI 1096 GCCCCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 738 AGATCT A^GATCT
BsaBI 733 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 930 GACGCC G[GA]^CG[CT]C
BsaWI 849 TCCGGA [AT]^CCGG[AT]
BseAI 849 TCCGGA T^CCGGA
BsgI 188 GTGCAG GTGCAG(16/14)
BsoBI 1006 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1585 TGTACA T^GTACA
BspEI 849 TCCGGA T^CCGGA
BspHI 1848 TCATGA T^CATGA
BspMI 158 ACCTGC ACCTGC(4/8)
BsrBI 968 CCGCTC CCGCTC(-3/-3)
BsrGI 1585 TGTACA T^GTACA
BstAPI 808 GCAGGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1176 CCAGCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 108 GACAGGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT14I 1100 CCATGG C^C[AT][AT]GG
FbaI 2394 TGATCA T^GATCA
HaeII 648 AGCGCC [GA]GCGC^[CT]
Hin1I 930 GACGCC G[GA]^CG[CT]C
HincII 307 GTTAAC GT[CT]^[GA]AC
HindII 307 GTTAAC GT[CT]^[GA]AC
HindIII 1777 AAGCTT A^AGCTT
HpaI 307 GTTAAC GTT^AAC
KpnI 137 GGTACC GGTAC^C
MaeIII 1012 GTTAC ^GT[ACGT]AC
MamI 733 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 849 TCCGGA T^CCGGA
NcoI 1100 CCATGG C^CATGG
PflMI 1275 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 478 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2293 ATTAAT AT^TAAT
RcaI 1848 TCATGA T^CATGA
SbfI 1111 CCTGCAGG CCTGCA^GG
ScaI 1871 AGTACT AGT^ACT
SmaI 1006 CCCGGG CCC^GGG
Sse8387I 1111 CCTGCAGG CCTGCA^GG
SspBI 1585 TGTACA T^GTACA
SspI 2603 AATATT AAT^ATT
StyI 1100 CCATGG C^C[AT][AT]GG
Van91I 1275 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1006 CCCGGG C^CCGGG
XmaI 1006 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AccII 72 CGCG CG^CG
212 CGCG
AhdI 18 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1368 GACTCTCAGTC
Alw44I 283 GTGCAC G^TGCAC
1685 GTGCAC
ApaLI 283 GTGCAC G^TGCAC
1685 GTGCAC
Asp700I 331 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
541 GAACCAGTTC
AspEI 18 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1368 GACTCTCAGTC
BbsI 607 GAAGAC GAAGAC(2/6)
1561 GAAGAC
BfaI 724 CTAG C^TAG
1920 CTAG
BlpI 1069 GCTCAGC GC^T[ACGT]AGC
1240 GCTCAGC
BpmI 166 CTGGAG CTGGAG(16/14)
2127 CTGGAG
Bpu1102I 1069 GCTCAGC GC^T[ACGT]AGC
1240 GCTCAGC
BpuAI 607 GAAGAC GAAGAC(2/6)
1561 GAAGAC
BsaI 400 GAGACC GGTCTC(1/5)
51 GGTCTC
BseRI 64 GAGGAG GAGGAG(10/8)
169 GAGGAG
BsmI 2151 GAATGC GAATGC(1/-1)
248 GCATTC
BsrFI 91 GCCGGC [GA]^CCGG[CT]
817 GCCGGC
BstUI 72 CGCG CG^CG
212 CGCG
CelII 1069 GCTCAGC GC^T[ACGT]AGC
1240 GCTCAGC
CfoI 71 GCGC GCG^C
649 GCGC
Cfr10I 91 GCCGGC [GA]^CCGG[CT]
817 GCCGGC
DraI 1823 TTTAAA TTT^AAA
2336 TTTAAA
DraII 1785 AGGTCCT [GA]G^G[ACGT]CC[CT]
1899 GGGTCCT
DsaI 1100 CCATGG C^C[GA][CT]GG
2121 CCGTGG
Eam1105I 18 GACCGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1368 GACTCTCAGTC
EarI 1423 CTCTTC CTCTTC(1/4)
1974 CTCTTC
EcoNI 723 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2102 CCTTCAGTAGG
EcoO109I 1785 AGGTCCT [GA]G^G[ACGT]CC[CT]
1899 GGGTCCT
HgaI 930 GACGC GACGC(5/10)
73 GCGTC
HhaI 71 GCGC GCG^C
649 GCGC
HinP1I 71 GCGC G^CGC
649 GCGC
Ksp632I 1423 CTCTTC CTCTTC(1/4)
1974 CTCTTC
MaeI 724 CTAG C^TAG
1920 CTAG
MspA1I 428 CAGCTG C[AC]G^C[GT]G
1246 CAGCTG
MvnI 72 CGCG CG^CG
212 CGCG
NaeI 91 GCCGGC GCC^GGC
817 GCCGGC
NgoMIV 91 GCCGGC G^CCGGC
817 GCCGGC
PpuMI 1785 AGGTCCT [GA]G^G[AT]CC[CT]
1899 GGGTCCT
PstI 806 CTGCAG CTGCA^G
1112 CTGCAG
PvuII 428 CAGCTG CAG^CTG
1246 CAGCTG
SphI 344 GCATGC GCATG^C
1130 GCATGC
XmnI 331 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
541 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BalI
BanI BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XcmI
XhoII