##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BamHI BbrPI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRV FseI FspI HindIII
HpaI KpnI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SpeI Sse8387I StuI XbaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AlwNI 706 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 833 AGCGCT AGC^GCT
AspHI 405 GTGCTC G[AT]GC[AT]^C
BalI 886 TGGCCA TGG^CCA
BanI 239 GGCGCC G^G[CT][GA]CC
BbeI 239 GGCGCC GGCGC^C
BbvCI 791 GCTGAGG CCTCAGC(-5/-2)
BclI 1175 TGATCA T^GATCA
BpmI 682 CTCCAG CTGGAG(16/14)
BsaAI 1961 TACGTA [CT]AC^GT[GA]
BsaI 211 GGTCTC GGTCTC(1/5)
BsiHKAI 405 GTGCTC G[AT]GC[AT]^C
BsmI 751 GAATGC GAATGC(1/-1)
Bsp1407I 2054 TGTACA T^GTACA
BsrDI 2286 GCAATG GCAATG(2/0)
BsrGI 2054 TGTACA T^GTACA
BstYI 345 AGATCC [GA]^GATC[CT]
DraII 1574 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eco47III 833 AGCGCT AGC^GCT
EcoO109I 1574 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1145 GAATTC G^AATTC
FauI 80 CCCGC CCCGC(4/6)
FbaI 1175 TGATCA T^GATCA
HincII 368 GTCAAC GT[CT]^[GA]AC
HindII 368 GTCAAC GT[CT]^[GA]AC
KasI 239 GGCGCC G^GCGCC
KspI 42 CCGCGG CCGC^GG
MflI 345 AGATCC [GA]^GATC[CT]
MluNI 886 TGGCCA TGG^CCA
MscI 886 TGGCCA TGG^CCA
NarI 239 GGCGCC GG^CGCC
NcoI 999 CCATGG C^CATGG
PpuMI 1574 AGGTCCT [GA]G^G[AT]CC[CT]
SacII 42 CCGCGG CCGC^GG
ScaI 1296 AGTACT AGT^ACT
SfoI 239 GGCGCC GGC^GCC
SmlI 541 CTTGAG C^T[CT][GA]AG
SnaBI 1961 TACGTA TAC^GTA
SspBI 2054 TGTACA T^GTACA
SwaI 1556 ATTTAAAT ATTT^AAAT
XhoII 345 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 43 CGCG CG^CG
70 CGCG
AcyI 239 GGCGCC G[GA]^CG[CT]C
299 GACGCC
AspI 320 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1004 GACATTGTC
BanII 331 GAGCCC G[GA]GC[CT]^C
573 GAGCCC
BbsI 1088 GAAGAC GAAGAC(2/6)
147 GTCTTC
BpuAI 1088 GAAGAC GAAGAC(2/6)
147 GTCTTC
BsaHI 239 GGCGCC G[GA]^CG[CT]C
299 GACGCC
BseRI 224 CTCCTC GAGGAG(10/8)
14 GAGGAG
BsgI 508 GTGCAG GTGCAG(16/14)
1841 GTGCAG
BsmAI 639 GAGAC GTCTC(1/5)
212 GTCTC
BspMI 1074 ACCTGC ACCTGC(4/8)
1343 ACCTGC
BsrBI 243 CCGCTC CCGCTC(-3/-3)
870 CCGCTC
BstUI 43 CGCG CG^CG
70 CGCG
BstXI 183 CCAGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
756 CCAGCAACATGG
DsaI 42 CCGCGG C^C[GA][CT]GG
999 CCATGG
EaeI 886 TGGCCA [CT]^GGCC[GA]
1021 TGGCCG
EcoT22I 1595 ATGCAT ATGCA^T
2249 ATGCAT
Hin1I 239 GGCGCC G[GA]^CG[CT]C
299 GACGCC
MaeII 321 ACGT A^CGT
1962 ACGT
MvnI 43 CGCG CG^CG
70 CGCG
NsiI 1595 ATGCAT ATGCA^T
2249 ATGCAT
PflFI 320 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1004 GACATTGTC
PflMI 558 CCATAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1930 CCACTGAATGG
PleI 491 GACTC GAGTC(4/5)
380 GAGTC
PstI 665 CTGCAG CTGCA^G
1076 CTGCAG
PvuII 392 CAGCTG CAG^CTG
1621 CAGCTG
SphI 1420 GCATGC GCATG^C
1597 GCATGC
SspI 1553 AATATT AAT^ATT
2131 AATATT
TaqI 571 TCGA T^CGA
637 TCGA
Tth111I 320 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1004 GACATTGTC
TthHB8I 571 TCGA T^CGA
637 TCGA
Van91I 558 CCATAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1930 CCACTGAATGG
XcmI 341 CCAGAGATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
962 CCAGACTGGATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BcnI BfaI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI