################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbrPI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRV FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SpeI Sse8387I StuI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AlwNI 706 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 833 AGCGCT AGC^GCT AspHI 405 GTGCTC G[AT]GC[AT]^C BalI 886 TGGCCA TGG^CCA BanI 239 GGCGCC G^G[CT][GA]CC BbeI 239 GGCGCC GGCGC^C BbvCI 791 GCTGAGG CCTCAGC(-5/-2) BclI 1175 TGATCA T^GATCA BpmI 682 CTCCAG CTGGAG(16/14) BsaAI 1961 TACGTA [CT]AC^GT[GA] BsaI 211 GGTCTC GGTCTC(1/5) BsiHKAI 405 GTGCTC G[AT]GC[AT]^C BsmI 751 GAATGC GAATGC(1/-1) Bsp1407I 2054 TGTACA T^GTACA BsrDI 2286 GCAATG GCAATG(2/0) BsrGI 2054 TGTACA T^GTACA BstYI 345 AGATCC [GA]^GATC[CT] DraII 1574 AGGTCCT [GA]G^G[ACGT]CC[CT] Eco47III 833 AGCGCT AGC^GCT EcoO109I 1574 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRI 1145 GAATTC G^AATTC FauI 80 CCCGC CCCGC(4/6) FbaI 1175 TGATCA T^GATCA HincII 368 GTCAAC GT[CT]^[GA]AC HindII 368 GTCAAC GT[CT]^[GA]AC KasI 239 GGCGCC G^GCGCC KspI 42 CCGCGG CCGC^GG MflI 345 AGATCC [GA]^GATC[CT] MluNI 886 TGGCCA TGG^CCA MscI 886 TGGCCA TGG^CCA NarI 239 GGCGCC GG^CGCC NcoI 999 CCATGG C^CATGG PpuMI 1574 AGGTCCT [GA]G^G[AT]CC[CT] SacII 42 CCGCGG CCGC^GG ScaI 1296 AGTACT AGT^ACT SfoI 239 GGCGCC GGC^GCC SmlI 541 CTTGAG C^T[CT][GA]AG SnaBI 1961 TACGTA TAC^GTA SspBI 2054 TGTACA T^GTACA SwaI 1556 ATTTAAAT ATTT^AAAT XhoII 345 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 43 CGCG CG^CG 70 CGCG AcyI 239 GGCGCC G[GA]^CG[CT]C 299 GACGCC AspI 320 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1004 GACATTGTC BanII 331 GAGCCC G[GA]GC[CT]^C 573 GAGCCC BbsI 1088 GAAGAC GAAGAC(2/6) 147 GTCTTC BpuAI 1088 GAAGAC GAAGAC(2/6) 147 GTCTTC BsaHI 239 GGCGCC G[GA]^CG[CT]C 299 GACGCC BseRI 224 CTCCTC GAGGAG(10/8) 14 GAGGAG BsgI 508 GTGCAG GTGCAG(16/14) 1841 GTGCAG BsmAI 639 GAGAC GTCTC(1/5) 212 GTCTC BspMI 1074 ACCTGC ACCTGC(4/8) 1343 ACCTGC BsrBI 243 CCGCTC CCGCTC(-3/-3) 870 CCGCTC BstUI 43 CGCG CG^CG 70 CGCG BstXI 183 CCAGCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 756 CCAGCAACATGG DsaI 42 CCGCGG C^C[GA][CT]GG 999 CCATGG EaeI 886 TGGCCA [CT]^GGCC[GA] 1021 TGGCCG EcoT22I 1595 ATGCAT ATGCA^T 2249 ATGCAT Hin1I 239 GGCGCC G[GA]^CG[CT]C 299 GACGCC MaeII 321 ACGT A^CGT 1962 ACGT MvnI 43 CGCG CG^CG 70 CGCG NsiI 1595 ATGCAT ATGCA^T 2249 ATGCAT PflFI 320 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1004 GACATTGTC PflMI 558 CCATAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1930 CCACTGAATGG PleI 491 GACTC GAGTC(4/5) 380 GAGTC PstI 665 CTGCAG CTGCA^G 1076 CTGCAG PvuII 392 CAGCTG CAG^CTG 1621 CAGCTG SphI 1420 GCATGC GCATG^C 1597 GCATGC SspI 1553 AATATT AAT^ATT 2131 AATATT TaqI 571 TCGA T^CGA 637 TCGA Tth111I 320 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1004 GACATTGTC TthHB8I 571 TCGA T^CGA 637 TCGA Van91I 558 CCATAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1930 CCACTGAATGG XcmI 341 CCAGAGATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 962 CCAGACTGGATATGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AvaII BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI