##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BclI BfrI BglII BlnI BseAI
BsgI BsiWI BspDI BspEI BspHI
BspLU11I Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MfeI MluI MroI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SpeI SphI Sse8387I
SspI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 1910 GTCTAC GT^[AC][GT]AC
Aor51HI 1634 AGCGCT AGC^GCT
ApaI 756 GGGCCC GGGCC^C
AspHI 2001 GTGCTC G[AT]GC[AT]^C
BalI 940 TGGCCA TGG^CCA
BbeI 952 GGCGCC GGCGC^C
BcgI 29 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 511 GTATCC GTATCC(6/5)
BlpI 1952 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1952 GCTCAGC GC^T[ACGT]AGC
BsaBI 650 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 909 GAGACC GGTCTC(1/5)
BsaWI 1540 ACCGGA [AT]^CCGG[AT]
BsiEI 1814 CGGTCG CG[GA][CT]^CG
BsiHKAI 2001 GTGCTC G[AT]GC[AT]^C
BsmBI 505 GAGACG CGTCTC(1/5)
BsmFI 1891 GTCCC GGGAC(10/14)
BsrBI 50 CCGCTC CCGCTC(-3/-3)
BsrDI 1686 CATTGC GCAATG(2/0)
BsrFI 112 GCCGGC [GA]^CCGG[CT]
BssHII 285 GCGCGC G^CGCGC
BssSI 1332 CACGAG CACGAG(-5/-1)
BstEII 733 GGTTACC G^GT[ACGT]ACC
BstPI 733 GGTTACC G^GT[ACGT]ACC
CelII 1952 GCTCAGC GC^T[ACGT]AGC
Cfr10I 112 GCCGGC [GA]^CCGG[CT]
Eco47III 1634 AGCGCT AGC^GCT
EcoO65I 733 GGTTACC G^GT[ACGT]ACC
KasI 952 GGCGCC G^GCGCC
MamI 650 GATAGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 940 TGGCCA TGG^CCA
MscI 940 TGGCCA TGG^CCA
NaeI 112 GCCGGC GCC^GGC
NarI 952 GGCGCC GG^CGCC
NgoMIV 112 GCCGGC G^CCGGC
PflMI 488 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 993 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 820 AGTACT AGT^ACT
SfoI 952 GGCGCC GGC^GCC
SnaBI 395 TACGTA TAC^GTA
Van91I 488 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1973 CCAGGCTGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 952 GGCGCC G[GA]^CG[CT]C
1323 GACGCC
AvaII 1625 GGACC G^G[AT]CC
1980 GGTCC
BanII 16 GGGCTC G[GA]GC[CT]^C
756 GGGCCC
BglI 98 GCCGTTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1407 GCCATCGAGGC
BsaAI 84 TACGTG [CT]AC^GT[GA]
395 TACGTA
BsaHI 952 GGCGCC G[GA]^CG[CT]C
1323 GACGCC
BsmAI 505 GAGAC GTCTC(1/5)
909 GAGAC
BsmI 586 GCATTC GAATGC(1/-1)
1416 GCATTC
Bsp1407I 926 TGTACA T^GTACA
1535 TGTACA
BsrGI 926 TGTACA T^GTACA
1535 TGTACA
BsrI 683 ACTGG ACTGG(1/-1)
1907 CCAGT
BstXI 1171 CCATCAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1465 CCAAGGGCCTGG
BstYI 331 AGATCC [GA]^GATC[CT]
610 AGATCC
HgaI 148 GACGC GACGC(5/10)
1323 GACGC
Hin1I 952 GGCGCC G[GA]^CG[CT]C
1323 GACGCC
MflI 331 AGATCC [GA]^GATC[CT]
610 AGATCC
MseI 268 TTAA T^TAA
2048 TTAA
PaeR7I 1260 CTCGAG C^TCGAG
1779 CTCGAG
SmaI 196 CCCGGG CCC^GGG
947 CCCGGG
SspBI 926 TGTACA T^GTACA
1535 TGTACA
StuI 569 AGGCCT AGG^CCT
1883 AGGCCT
TfiI 1130 GATTC G^A[AT]TC
1938 GAATC
Tru9I 268 TTAA T^TAA
2048 TTAA
Tsp509I 1503 AATT ^AATT
2012 AATT
XhoI 1260 CTCGAG C^TCGAG
1779 CTCGAG
XhoII 331 AGATCC [GA]^GATC[CT]
610 AGATCC
XmaCI 196 CCCGGG C^CCGGG
947 CCCGGG
XmaI 196 CCCGGG C^CCGGG
947 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI BanI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsiYI BslI BsoBI
Bsp1286I BspMI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I