##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Aor51HI ApaI AscI
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbsI BcgI
BclI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspMI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII CfoI Cfr10I ClaI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
KasI KpnI MamI MfeI MluI
MluNI MscI MunI NaeI NarI
NcoI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI Psp1406I PvuI
SacI SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI StuI
StyI SwaI TfiI Tth111I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 379 GTCTAC GT^[AC][GT]AC
AccIII 230 TCCGGA T^CCGGA
AflIII 2414 ACATGT A^C[GA][CT]GT
Alw44I 800 GTGCAC G^TGCAC
AocI 1230 CCTTAGG CC^T[ACGT]AGG
ApaLI 800 GTGCAC G^TGCAC
AseI 2441 ATTAAT AT^TAAT
AsnI 2441 ATTAAT AT^TAAT
AspHI 800 GTGCAC G[AT]GC[AT]^C
AvaI 62 CCCGGG C^[CT]CG[GA]G
BbrPI 639 CACGTG CAC^GTG
BbvCI 1728 GCTGAGG CCTCAGC(-5/-2)
BglII 944 AGATCT A^GATCT
BpmI 958 CTCCAG CTGGAG(16/14)
BsaWI 230 TCCGGA [AT]^CCGG[AT]
BseAI 230 TCCGGA T^CCGGA
BseRI 203 CTCCTC GAGGAG(10/8)
BsiEI 300 CGACCG CG[GA][CT]^CG
BsiHKAI 800 GTGCAC G[AT]GC[AT]^C
BsoBI 62 CCCGGG C^[CT]CG[GA]G
BspEI 230 TCCGGA T^CCGGA
BspHI 2224 TCATGA T^CATGA
BspLU11I 2414 ACATGT A^CATGT
BsrBI 77 GAGCGG CCGCTC(-3/-3)
BsrDI 1225 CATTGC GCAATG(2/0)
BssSI 286 CACGAG CACGAG(-5/-1)
Bsu36I 1230 CCTTAGG CC^T[ACGT]AGG
CpoI 165 CGGACCG CG^G[AT]CCG
DraII 563 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 1230 CCTTAGG CC^T[ACGT]AGG
EcoO109I 563 GGGACCC [GA]G^G[ACGT]CC[CT]
FauI 35 CCCGC CCCGC(4/6)
HincII 985 GTTAAC GT[CT]^[GA]AC
HindII 985 GTTAAC GT[CT]^[GA]AC
HpaI 985 GTTAAC GTT^AAC
MroI 230 TCCGGA T^CCGGA
PmaCI 639 CACGTG CAC^GTG
PmlI 639 CACGTG CAC^GTG
PpuMI 563 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2441 ATTAAT AT^TAAT
PstI 833 CTGCAG CTGCA^G
RcaI 2224 TCATGA T^CATGA
RsrII 165 CGGACCG CG^G[AT]CCG
ScaI 2273 AGTACT AGT^ACT
SmaI 62 CCCGGG CCC^GGG
SphI 587 GCATGC GCATG^C
TaqI 573 TCGA T^CGA
TthHB8I 573 TCGA T^CGA
XcmI 733 CCACCATTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 62 CCCGGG C^CCGGG
XmaI 62 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
Asp700I 361 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2149 GAAGTGTTTC
AvaII 166 GGACC G^G[AT]CC
564 GGACC
BanI 92 GGCACC G^G[CT][GA]CC
1596 GGCACC
BciVI 1701 GGATAC GTATCC(6/5)
2305 GTATCC
BsaAI 639 CACGTG [CT]AC^GT[GA]
855 TACGTG
BsmFI 322 GGGAC GGGAC(10/14)
563 GGGAC
BstXI 733 CCACCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
736 CCATTTTGGTGG
HindIII 1182 AAGCTT A^AGCTT
1966 AAGCTT
KspI 36 CCGCGG CCGC^GG
169 CCGCGG
PvuII 418 CAGCTG CAG^CTG
993 CAGCTG
SacII 36 CCGCGG CCGC^GG
169 CCGCGG
Tsp45I 760 GTCAC ^GT[GC]AC
2065 GTGAC
XmnI 361 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2149 GAAGTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI BanII BbvI
BcnI BfaI BmyI BsaI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DsaI EarI EcoRI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NheI NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspI Tru9I TseI Tsp509I
TspRI XhoII