##################################
# NO CUT ENZYMES (  120 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AvaI        
AviII       AvrII       BbeI        BbrPI       BcgI        
BciVI       BclI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsiWI       BsmBI       BsmI        BsoBI       BspDI       
BspLU11I    BspMI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
Eam1105I    Eco47III    Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        Hin1I       
HincII      HindII      HpaI        KasI        KpnI        
KspI        MaeI        MfeI        MluI        MseI        
MunI        MvnI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tru9I       
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccIII       1728  TCCGGA               T^CCGGA
ApaI         1596  GGGCCC               GGGCC^C
Asp700I       188  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          582  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI        1557  GCTGAGG              CCTCAGC(-5/-2)
BsaBI        1226  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI           34  GAGACC               GGTCTC(1/5)
BsaWI        1728  TCCGGA               [AT]^CCGG[AT]
BseAI        1728  TCCGGA               T^CCGGA
BseRI        1673  CTCCTC               GAGGAG(10/8)
BsgI         1410  CTGCAC               GTGCAG(16/14)
BsiEI         719  CGGCCG               CG[GA][CT]^CG
BspEI        1728  TCCGGA               T^CCGGA
BspHI         931  TCATGA               T^CATGA
BsrFI         625  GCCGGC               [GA]^CCGG[CT]
BstEII       1450  GGTGACC              G^GT[ACGT]ACC
BstPI        1450  GGTGACC              G^GT[ACGT]ACC
CfoI         1043  GCGC                 GCG^C
Cfr10I        625  GCCGGC               [GA]^CCGG[CT]
DraIII        960  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          579  GACGACATCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          642  CCGTGG               C^C[GA][CT]GG
EagI          719  CGGCCG               C^GGCCG
EarI          921  CTCTTC               CTCTTC(1/4)
EclXI         719  CGGCCG               C^GGCCG
Eco52I        719  CGGCCG               C^GGCCG
EcoNI         113  CCTGTCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1450  GGTGACC              G^GT[ACGT]ACC
HaeII        1042  AGCGCC               [GA]GCGC^[CT]
HhaI         1043  GCGC                 GCG^C
HinP1I       1043  GCGC                 G^CGC
HindIII       189  AAGCTT               A^AGCTT
Ksp632I       921  CTCTTC               CTCTTC(1/4)
MamI         1226  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI         1728  TCCGGA               T^CCGGA
NaeI          625  GCCGGC               GCC^GGC
NgoMIV        625  GCCGGC               G^CCGGC
PflFI         582  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI          931  TCATGA               T^CATGA
TfiI          990  GAATC                G^A[AT]TC
Tth111I       582  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          188  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcsI         1769  AAATTT               [GA]^AATT[CT]
             1820  AAATTT              
AlwNI         852  CAGAACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1456  CAGACACTG           
ApoI         1769  AAATTT               [GA]^AATT[CT]
             1820  AAATTT              
AspHI        1623  GAGCTC               G[AT]GC[AT]^C
             1739  GAGCTC              
BamHI          57  GGATCC               G^GATCC
             1725  GGATCC              
BbsI          999  GAAGAC               GAAGAC(2/6)
              736  GTCTTC              
BglI          438  GCCCGTAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1476  GCCAAGACGGC         
BpuAI         999  GAAGAC               GAAGAC(2/6)
              736  GTCTTC              
BsiHKAI      1623  GAGCTC               G[AT]GC[AT]^C
             1739  GAGCTC              
Bsp1407I     1711  TGTACA               T^GTACA
             1745  TGTACA              
BsrDI         679  CATTGC               GCAATG(2/0)
              781  GCAATG              
BsrGI        1711  TGTACA               T^GTACA
             1745  TGTACA              
HgaI          242  GACGC                GACGC(5/10)
              743  GCGTC               
MslI          590  CACCCGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1467  CACTGCAGTG          
MspA1I       1652  CAGCTG               C[AC]G^C[GT]G
             1705  CAGCTG              
PflMI         959  CCACATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1423  CCAAGGACTGG         
PpuMI         321  GGGTCCC              [GA]G^G[AT]CC[CT]
             1110  GGGACCC             
PvuII        1652  CAGCTG               CAG^CTG
             1705  CAGCTG              
SacI         1623  GAGCTC               GAGCT^C
             1739  GAGCTC              
SfaNI        1018  GCATC                GCATC(5/9)
             1258  GCATC               
SspBI        1711  TGTACA               T^GTACA
             1745  TGTACA              
StuI         1585  AGGCCT               AGG^CCT
             1669  AGGCCT              
Van91I        959  CCACATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1423  CCAAGGACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   73 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrBI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfcI        SmlI        
StyI        TaqI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII