##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsiWI BsmBI BsmI BsoBI BspDI
BspLU11I BspMI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeI MfeI MluI MseI
MunI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccIII 1728 TCCGGA T^CCGGA
ApaI 1596 GGGCCC GGGCC^C
Asp700I 188 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 582 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 1557 GCTGAGG CCTCAGC(-5/-2)
BsaBI 1226 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 34 GAGACC GGTCTC(1/5)
BsaWI 1728 TCCGGA [AT]^CCGG[AT]
BseAI 1728 TCCGGA T^CCGGA
BseRI 1673 CTCCTC GAGGAG(10/8)
BsgI 1410 CTGCAC GTGCAG(16/14)
BsiEI 719 CGGCCG CG[GA][CT]^CG
BspEI 1728 TCCGGA T^CCGGA
BspHI 931 TCATGA T^CATGA
BsrFI 625 GCCGGC [GA]^CCGG[CT]
BstEII 1450 GGTGACC G^GT[ACGT]ACC
BstPI 1450 GGTGACC G^GT[ACGT]ACC
CfoI 1043 GCGC GCG^C
Cfr10I 625 GCCGGC [GA]^CCGG[CT]
DraIII 960 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 579 GACGACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 642 CCGTGG C^C[GA][CT]GG
EagI 719 CGGCCG C^GGCCG
EarI 921 CTCTTC CTCTTC(1/4)
EclXI 719 CGGCCG C^GGCCG
Eco52I 719 CGGCCG C^GGCCG
EcoNI 113 CCTGTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1450 GGTGACC G^GT[ACGT]ACC
HaeII 1042 AGCGCC [GA]GCGC^[CT]
HhaI 1043 GCGC GCG^C
HinP1I 1043 GCGC G^CGC
HindIII 189 AAGCTT A^AGCTT
Ksp632I 921 CTCTTC CTCTTC(1/4)
MamI 1226 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1728 TCCGGA T^CCGGA
NaeI 625 GCCGGC GCC^GGC
NgoMIV 625 GCCGGC G^CCGGC
PflFI 582 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 931 TCATGA T^CATGA
TfiI 990 GAATC G^A[AT]TC
Tth111I 582 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 188 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 1769 AAATTT [GA]^AATT[CT]
1820 AAATTT
AlwNI 852 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1456 CAGACACTG
ApoI 1769 AAATTT [GA]^AATT[CT]
1820 AAATTT
AspHI 1623 GAGCTC G[AT]GC[AT]^C
1739 GAGCTC
BamHI 57 GGATCC G^GATCC
1725 GGATCC
BbsI 999 GAAGAC GAAGAC(2/6)
736 GTCTTC
BglI 438 GCCCGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1476 GCCAAGACGGC
BpuAI 999 GAAGAC GAAGAC(2/6)
736 GTCTTC
BsiHKAI 1623 GAGCTC G[AT]GC[AT]^C
1739 GAGCTC
Bsp1407I 1711 TGTACA T^GTACA
1745 TGTACA
BsrDI 679 CATTGC GCAATG(2/0)
781 GCAATG
BsrGI 1711 TGTACA T^GTACA
1745 TGTACA
HgaI 242 GACGC GACGC(5/10)
743 GCGTC
MslI 590 CACCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1467 CACTGCAGTG
MspA1I 1652 CAGCTG C[AC]G^C[GT]G
1705 CAGCTG
PflMI 959 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1423 CCAAGGACTGG
PpuMI 321 GGGTCCC [GA]G^G[AT]CC[CT]
1110 GGGACCC
PvuII 1652 CAGCTG CAG^CTG
1705 CAGCTG
SacI 1623 GAGCTC GAGCT^C
1739 GAGCTC
SfaNI 1018 GCATC GCATC(5/9)
1258 GCATC
SspBI 1711 TGTACA T^GTACA
1745 TGTACA
StuI 1585 AGGCCT AGG^CCT
1669 AGGCCT
Van91I 959 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1423 CCAAGGACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BalI BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrBI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII