################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BbeI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsiWI BsmBI BsmI BsoBI BspDI BspLU11I BspMI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI Eam1105I Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MaeI MfeI MluI MseI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 1728 TCCGGA T^CCGGA ApaI 1596 GGGCCC GGGCC^C Asp700I 188 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 582 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbvCI 1557 GCTGAGG CCTCAGC(-5/-2) BsaBI 1226 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 34 GAGACC GGTCTC(1/5) BsaWI 1728 TCCGGA [AT]^CCGG[AT] BseAI 1728 TCCGGA T^CCGGA BseRI 1673 CTCCTC GAGGAG(10/8) BsgI 1410 CTGCAC GTGCAG(16/14) BsiEI 719 CGGCCG CG[GA][CT]^CG BspEI 1728 TCCGGA T^CCGGA BspHI 931 TCATGA T^CATGA BsrFI 625 GCCGGC [GA]^CCGG[CT] BstEII 1450 GGTGACC G^GT[ACGT]ACC BstPI 1450 GGTGACC G^GT[ACGT]ACC CfoI 1043 GCGC GCG^C Cfr10I 625 GCCGGC [GA]^CCGG[CT] DraIII 960 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 579 GACGACATCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 642 CCGTGG C^C[GA][CT]GG EagI 719 CGGCCG C^GGCCG EarI 921 CTCTTC CTCTTC(1/4) EclXI 719 CGGCCG C^GGCCG Eco52I 719 CGGCCG C^GGCCG EcoNI 113 CCTGTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1450 GGTGACC G^GT[ACGT]ACC HaeII 1042 AGCGCC [GA]GCGC^[CT] HhaI 1043 GCGC GCG^C HinP1I 1043 GCGC G^CGC HindIII 189 AAGCTT A^AGCTT Ksp632I 921 CTCTTC CTCTTC(1/4) MamI 1226 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1728 TCCGGA T^CCGGA NaeI 625 GCCGGC GCC^GGC NgoMIV 625 GCCGGC G^CCGGC PflFI 582 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 931 TCATGA T^CATGA TfiI 990 GAATC G^A[AT]TC Tth111I 582 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 188 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 1769 AAATTT [GA]^AATT[CT] 1820 AAATTT AlwNI 852 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 1456 CAGACACTG ApoI 1769 AAATTT [GA]^AATT[CT] 1820 AAATTT AspHI 1623 GAGCTC G[AT]GC[AT]^C 1739 GAGCTC BamHI 57 GGATCC G^GATCC 1725 GGATCC BbsI 999 GAAGAC GAAGAC(2/6) 736 GTCTTC BglI 438 GCCCGTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1476 GCCAAGACGGC BpuAI 999 GAAGAC GAAGAC(2/6) 736 GTCTTC BsiHKAI 1623 GAGCTC G[AT]GC[AT]^C 1739 GAGCTC Bsp1407I 1711 TGTACA T^GTACA 1745 TGTACA BsrDI 679 CATTGC GCAATG(2/0) 781 GCAATG BsrGI 1711 TGTACA T^GTACA 1745 TGTACA HgaI 242 GACGC GACGC(5/10) 743 GCGTC MslI 590 CACCCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1467 CACTGCAGTG MspA1I 1652 CAGCTG C[AC]G^C[GT]G 1705 CAGCTG PflMI 959 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1423 CCAAGGACTGG PpuMI 321 GGGTCCC [GA]G^G[AT]CC[CT] 1110 GGGACCC PvuII 1652 CAGCTG CAG^CTG 1705 CAGCTG SacI 1623 GAGCTC GAGCT^C 1739 GAGCTC SfaNI 1018 GCATC GCATC(5/9) 1258 GCATC SspBI 1711 TGTACA T^GTACA 1745 TGTACA StuI 1585 AGGCCT AGG^CCT 1669 AGGCCT Van91I 959 CCACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1423 CCAAGGACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII