##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AvaII AviII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BcnI BfrI BglI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AflIII 67 ACATGT A^C[GA][CT]GT
AlwI 1815 GATCC GGATC(4/5)
Asp700I 115 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1594 GAGCAC G[AT]GC[AT]^C
AvrII 329 CCTAGG C^CTAGG
BanII 651 GGGCTC G[GA]GC[CT]^C
BbsI 762 GTCTTC GAAGAC(2/6)
BciVI 1721 GGATAC GTATCC(6/5)
BlnI 329 CCTAGG C^CTAGG
BpuAI 762 GTCTTC GAAGAC(2/6)
BsiHKAI 1594 GAGCAC G[AT]GC[AT]^C
BsiYI 418 CCATTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 418 CCATTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 14 GGGAC GGGAC(10/14)
BspHI 1061 TCATGA T^CATGA
BspLU11I 67 ACATGT A^CATGT
BsrDI 1624 GCAATG GCAATG(2/0)
BstAPI 277 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 751 TTCGAA TT^CGAA
CfoI 51 GCGC GCG^C
DraIII 395 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 716 TGGCCG [CT]^GGCC[GA]
EarI 658 CTCTTC CTCTTC(1/4)
EcoT14I 329 CCTAGG C^C[AT][AT]GG
HapII 719 CCGG C^CGG
HhaI 51 GCGC GCG^C
HinP1I 51 GCGC G^CGC
HindIII 1078 AAGCTT A^AGCTT
HpaII 719 CCGG C^CGG
Ksp632I 658 CTCTTC CTCTTC(1/4)
MfeI 1372 CAATTG C^AATTG
MslI 1603 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 767 CAGCGG C[AC]G^C[GT]G
MspI 719 CCGG C^CGG
MunI 1372 CAATTG C^AATTG
NlaIV 721 GGAACC GG[ACGT]^[ACGT]CC
NspI 67 ACATGT [GA]CATG^[CT]
NspV 751 TTCGAA TT^CGAA
PflMI 418 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 436 CTGCAG CTGCA^G
RcaI 1061 TCATGA T^CATGA
SexAI 509 ACCAGGT A^CC[AT]GGT
SfaNI 600 GATGC GCATC(5/9)
SfuI 751 TTCGAA TT^CGAA
SmlI 1075 CTCAAG C^T[CT][GA]AG
SpeI 1725 ACTAGT A^CTAGT
StyI 329 CCTAGG C^C[AT][AT]GG
Van91I 418 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 115 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 769 GCGG CCGC(-3/-1)
1571 GCGG
BbvI 435 GCTGC GCAGC(8/12)
1138 GCTGC
BclI 860 TGATCA T^GATCA
1288 TGATCA
BglII 1906 AGATCT A^GATCT
1928 AGATCT
BmyI 651 GGGCTC G[AGT]GC[ACT]^C
1594 GAGCAC
BsaI 200 GAGACC GGTCTC(1/5)
854 GGTCTC
BsaJI 329 CCTAGG C^C[ACGT][ACGT]GG
838 CCTGGG
Bsp1286I 651 GGGCTC G[AGT]GC[ACT]^C
1594 GAGCAC
BsrI 1046 ACTGG ACTGG(1/-1)
44 CCAGT
BstYI 1906 AGATCT [GA]^GATC[CT]
1928 AGATCT
DrdI 318 GACACTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1354 GACTCATCTGTC
FbaI 860 TGATCA T^GATCA
1288 TGATCA
HaeIII 585 GGCC GG^CC
717 GGCC
MaeII 811 ACGT A^CGT
1745 ACGT
MflI 1906 AGATCT [GA]^GATC[CT]
1928 AGATCT
SspI 125 AATATT AAT^ATT
2013 AATATT
TseI 435 GCTGC G^C[AT]GC
1138 GCTGC
XhoII 1906 AGATCT [GA]^GATC[CT]
1928 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AcsI AfaI AluI ApoI AseI
AsnI BfaI BsmAI BssKI BstF5I
BstNI Cac8I DdeI DpnII DraI
EcoRII Fnu4HI FokI HinfI HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MvaI MwoI NdeII
NlaIII PleI PshBI RsaI Sau3AI
ScrFI SfcI TaqI TfiI Tru9I
Tsp45I Tsp509I TspRI TthHB8I