################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI AvaII AviII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BcnI BfrI BglI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AflIII 67 ACATGT A^C[GA][CT]GT AlwI 1815 GATCC GGATC(4/5) Asp700I 115 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1594 GAGCAC G[AT]GC[AT]^C AvrII 329 CCTAGG C^CTAGG BanII 651 GGGCTC G[GA]GC[CT]^C BbsI 762 GTCTTC GAAGAC(2/6) BciVI 1721 GGATAC GTATCC(6/5) BlnI 329 CCTAGG C^CTAGG BpuAI 762 GTCTTC GAAGAC(2/6) BsiHKAI 1594 GAGCAC G[AT]GC[AT]^C BsiYI 418 CCATTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 418 CCATTACTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 14 GGGAC GGGAC(10/14) BspHI 1061 TCATGA T^CATGA BspLU11I 67 ACATGT A^CATGT BsrDI 1624 GCAATG GCAATG(2/0) BstAPI 277 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 751 TTCGAA TT^CGAA CfoI 51 GCGC GCG^C DraIII 395 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 716 TGGCCG [CT]^GGCC[GA] EarI 658 CTCTTC CTCTTC(1/4) EcoT14I 329 CCTAGG C^C[AT][AT]GG HapII 719 CCGG C^CGG HhaI 51 GCGC GCG^C HinP1I 51 GCGC G^CGC HindIII 1078 AAGCTT A^AGCTT HpaII 719 CCGG C^CGG Ksp632I 658 CTCTTC CTCTTC(1/4) MfeI 1372 CAATTG C^AATTG MslI 1603 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 767 CAGCGG C[AC]G^C[GT]G MspI 719 CCGG C^CGG MunI 1372 CAATTG C^AATTG NlaIV 721 GGAACC GG[ACGT]^[ACGT]CC NspI 67 ACATGT [GA]CATG^[CT] NspV 751 TTCGAA TT^CGAA PflMI 418 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 436 CTGCAG CTGCA^G RcaI 1061 TCATGA T^CATGA SexAI 509 ACCAGGT A^CC[AT]GGT SfaNI 600 GATGC GCATC(5/9) SfuI 751 TTCGAA TT^CGAA SmlI 1075 CTCAAG C^T[CT][GA]AG SpeI 1725 ACTAGT A^CTAGT StyI 329 CCTAGG C^C[AT][AT]GG Van91I 418 CCATTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 115 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 769 GCGG CCGC(-3/-1) 1571 GCGG BbvI 435 GCTGC GCAGC(8/12) 1138 GCTGC BclI 860 TGATCA T^GATCA 1288 TGATCA BglII 1906 AGATCT A^GATCT 1928 AGATCT BmyI 651 GGGCTC G[AGT]GC[ACT]^C 1594 GAGCAC BsaI 200 GAGACC GGTCTC(1/5) 854 GGTCTC BsaJI 329 CCTAGG C^C[ACGT][ACGT]GG 838 CCTGGG Bsp1286I 651 GGGCTC G[AGT]GC[ACT]^C 1594 GAGCAC BsrI 1046 ACTGG ACTGG(1/-1) 44 CCAGT BstYI 1906 AGATCT [GA]^GATC[CT] 1928 AGATCT DrdI 318 GACACTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1354 GACTCATCTGTC FbaI 860 TGATCA T^GATCA 1288 TGATCA HaeIII 585 GGCC GG^CC 717 GGCC MaeII 811 ACGT A^CGT 1745 ACGT MflI 1906 AGATCT [GA]^GATC[CT] 1928 AGATCT SspI 125 AATATT AAT^ATT 2013 AATATT TseI 435 GCTGC G^C[AT]GC 1138 GCTGC XhoII 1906 AGATCT [GA]^GATC[CT] 1928 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AcsI AfaI AluI ApoI AseI AsnI BfaI BsmAI BssKI BstF5I BstNI Cac8I DdeI DpnII DraI EcoRII Fnu4HI FokI HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII PleI PshBI RsaI Sau3AI ScrFI SfcI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI TthHB8I