##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Aor51HI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BamHI BbeI BcgI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrGI BssHII Bst1107I BstBI BstZ17I
CelII ClaI CpoI Eam1105I Eco47III
EcoRI EcoRV FseI HindIII KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 2494 AAATTT [GA]^AATT[CT]
AcyI 1653 GGCGTC G[GA]^CG[CT]C
AflIII 910 ACATGT A^C[GA][CT]GT
Alw44I 230 GTGCAC G^TGCAC
ApaLI 230 GTGCAC G^TGCAC
ApoI 2494 AAATTT [GA]^AATT[CT]
AviII 126 TGCGCA TGC^GCA
BbrPI 1680 CACGTG CAC^GTG
BbvCI 1903 CCTCAGC CCTCAGC(-5/-2)
BciVI 1395 GGATAC GTATCC(6/5)
BclI 886 TGATCA T^GATCA
BfaI 2112 CTAG C^TAG
BsaAI 1680 CACGTG [CT]AC^GT[GA]
BsaHI 1653 GGCGTC G[GA]^CG[CT]C
BsiEI 1 CGGCCG CG[GA][CT]^CG
BspLU11I 910 ACATGT A^CATGT
BsrDI 1483 GCAATG GCAATG(2/0)
BstEII 674 GGTCACC G^GT[ACGT]ACC
BstPI 674 GGTCACC G^GT[ACGT]ACC
BstXI 470 CCACAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2542 TTTAAA TTT^AAA
DraIII 1077 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1 CGGCCG C^GGCCG
EclXI 1 CGGCCG C^GGCCG
Eco52I 1 CGGCCG C^GGCCG
EcoO65I 674 GGTCACC G^GT[ACGT]ACC
EcoT22I 861 ATGCAT ATGCA^T
FbaI 886 TGATCA T^GATCA
FspI 126 TGCGCA TGC^GCA
Hin1I 1653 GGCGTC G[GA]^CG[CT]C
HpaI 146 GTTAAC GTT^AAC
MaeI 2112 CTAG C^TAG
MspA1I 2027 CCGCTG C[AC]G^C[GT]G
NaeI 567 GCCGGC GCC^GGC
NgoMIV 567 GCCGGC G^CCGGC
NsiI 861 ATGCAT ATGCA^T
PleI 2467 GACTC GAGTC(4/5)
PmaCI 1680 CACGTG CAC^GTG
PmlI 1680 CACGTG CAC^GTG
PshAI 1352 GACCGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SspI 2560 AATATT AAT^ATT
SwaI 2541 ATTTAAAT ATTT^AAAT
TfiI 1974 GAATC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwI 1190 GATCC GGATC(4/5)
2167 GGATC
AocI 816 CCTGAGG CC^T[ACGT]AGG
2301 CCTCAGG
Asp700I 1004 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1457 GAAGCCCTTC
AvaI 33 CCCGGG C^[CT]CG[GA]G
1822 CCCGGG
BsgI 687 CTGCAC GTGCAG(16/14)
1647 CTGCAC
BsoBI 33 CCCGGG C^[CT]CG[GA]G
1822 CCCGGG
BsrFI 567 GCCGGC [GA]^CCGG[CT]
2357 GCCGGT
BstAPI 1274 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1640 GCACCTGCTGC
BstYI 1189 AGATCC [GA]^GATC[CT]
2167 GGATCT
Bsu36I 816 CCTGAGG CC^T[ACGT]AGG
2301 CCTCAGG
Cfr10I 567 GCCGGC [GA]^CCGG[CT]
2357 GCCGGT
DrdI 1148 GACGGACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1608 GACGTGGCCGTC
Eco81I 816 CCTGAGG CC^T[ACGT]AGG
2301 CCTCAGG
HaeII 564 AGCGCC [GA]GCGC^[CT]
759 AGCGCC
HincII 146 GTTAAC GT[CT]^[GA]AC
993 GTTGAC
HindII 146 GTTAAC GT[CT]^[GA]AC
993 GTTGAC
HinfI 1974 GAATC G^A[ACGT]TC
2467 GACTC
MflI 1189 AGATCC [GA]^GATC[CT]
2167 GGATCT
NcoI 1531 CCATGG C^CATGG
1633 CCATGG
NspI 910 ACATGT [GA]CATG^[CT]
1117 ACATGC
PstI 1756 CTGCAG CTGCA^G
2082 CTGCAG
SapI 1004 GAAGAGC GCTCTTC(1/4)
2401 GAAGAGC
ScaI 254 AGTACT AGT^ACT
316 AGTACT
SfcI 1756 CTGCAG C^T[GA][CT]AG
2082 CTGCAG
SmaI 33 CCCGGG CCC^GGG
1822 CCCGGG
StuI 820 AGGCCT AGG^CCT
2311 AGGCCT
TaqI 188 TCGA T^CGA
1105 TCGA
Tsp509I 1241 AATT ^AATT
2495 AATT
TthHB8I 188 TCGA T^CGA
1105 TCGA
XhoII 1189 AGATCC [GA]^GATC[CT]
2167 GGATCT
XmaCI 33 CCCGGG C^CCGGG
1822 CCCGGG
XmaI 33 CCCGGG C^CCGGG
1822 CCCGGG
XmnI 1004 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1457 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BssSI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
Tru9I TseI Tsp45I TspRI Van91I
XcmI