##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MfeI MluI
MluNI MroI MscI MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI StuI
SwaI Tth111I XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 393 GTCTAC GT^[AC][GT]AC
AcsI 417 AAATTT [GA]^AATT[CT]
Alw44I 408 GTGCAC G^TGCAC
ApaLI 408 GTGCAC G^TGCAC
ApoI 417 AAATTT [GA]^AATT[CT]
AspHI 408 GTGCAC G[AT]GC[AT]^C
AvaI 565 CCCGAG C^[CT]CG[GA]G
BamHI 52 GGATCC G^GATCC
BbvCI 1594 GCTGAGG CCTCAGC(-5/-2)
BglII 1458 AGATCT A^GATCT
BsaBI 357 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 897 GTGCAG GTGCAG(16/14)
BsiHKAI 408 GTGCAC G[AT]GC[AT]^C
BsoBI 565 CCCGAG C^[CT]CG[GA]G
BsrBI 847 CCGCTC CCGCTC(-3/-3)
BsrDI 1328 GCAATG GCAATG(2/0)
BstXI 786 CCAAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 38 GCGC GCG^C
EcoNI 423 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 702 GATATC GAT^ATC
HaeII 37 GGCGCT [GA]GCGC^[CT]
HhaI 38 GCGC GCG^C
HinP1I 38 GCGC G^CGC
MamI 357 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 611 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 1240 GCATGT [GA]CATG^[CT]
PflMI 263 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1469 GAGTC GAGTC(4/5)
PpuMI 141 AGGTCCC [GA]G^G[AT]CC[CT]
SbfI 1099 CCTGCAGG CCTGCA^GG
ScaI 1013 AGTACT AGT^ACT
SmlI 1017 CTCAAG C^T[CT][GA]AG
Sse8387I 1099 CCTGCAGG CCTGCA^GG
SspI 1110 AATATT AAT^ATT
TaqI 1544 TCGA T^CGA
TthHB8I 1544 TCGA T^CGA
Van91I 263 CCACACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1075 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AocI 153 CCTCAGG CC^T[ACGT]AGG
423 CCTGAGG
BanII 62 GAGCCC G[GA]GC[CT]^C
122 GGGCTC
BcnI 632 CCGGG CC^[GC]GG
833 CCGGG
BpmI 850 CTCCAG CTGGAG(16/14)
971 CTCCAG
BsrFI 148 GCCGGC [GA]^CCGG[CT]
594 GCCGGC
Bsu36I 153 CCTCAGG CC^T[ACGT]AGG
423 CCTGAGG
Cfr10I 148 GCCGGC [GA]^CCGG[CT]
594 GCCGGC
DraII 141 AGGTCCC [GA]G^G[ACGT]CC[CT]
1598 AGGCCCC
EarI 1213 CTCTTC CTCTTC(1/4)
1128 GAAGAG
Eco81I 153 CCTCAGG CC^T[ACGT]AGG
423 CCTGAGG
EcoO109I 141 AGGTCCC [GA]G^G[ACGT]CC[CT]
1598 AGGCCCC
Ksp632I 1213 CTCTTC CTCTTC(1/4)
1128 GAAGAG
MspA1I 624 CAGCTG C[AC]G^C[GT]G
900 CAGCTG
NaeI 148 GCCGGC GCC^GGC
594 GCCGGC
NciI 632 CCGGG CC^[GC]GG
833 CCGGG
NgoMIV 148 GCCGGC G^CCGGC
594 GCCGGC
PvuII 624 CAGCTG CAG^CTG
900 CAGCTG
Tsp509I 311 AATT ^AATT
418 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BfaI BmyI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I TspRI XhoII