##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AspEI
AvrII BbeI BbrPI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FseI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KspI MaeII MamI
MfeI MluI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 342 GGTACC G^GTACC
AcsI 1025 GAATTC [GA]^AATT[CT]
ApaI 15 GGGCCC GGGCC^C
ApoI 1025 GAATTC [GA]^AATT[CT]
AseI 1684 ATTAAT AT^TAAT
AsnI 1684 ATTAAT AT^TAAT
Asp700I 1530 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 342 GGTACC G^GTACC
AspI 1975 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 48 CTCGGG C^[CT]CG[GA]G
AviII 1310 TGCGCA TGC^GCA
BalI 1793 TGGCCA TGG^CCA
BamHI 477 GGATCC G^GATCC
BbvCI 1884 GCTGAGG CCTCAGC(-5/-2)
BciVI 1619 GTATCC GTATCC(6/5)
BclI 810 TGATCA T^GATCA
BglII 39 AGATCT A^GATCT
BsgI 1947 CTGCAC GTGCAG(16/14)
BsoBI 48 CTCGGG C^[CT]CG[GA]G
BsrDI 554 GCAATG GCAATG(2/0)
BsrFI 784 GCCGGT [GA]^CCGG[CT]
BssSI 1766 CACGAG CACGAG(-5/-1)
BstAPI 1916 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1735 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 784 GCCGGT [GA]^CCGG[CT]
EcoNI 354 CCTTCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1025 GAATTC G^AATTC
FbaI 810 TGATCA T^GATCA
FspI 1310 TGCGCA TGC^GCA
HindIII 148 AAGCTT A^AGCTT
KpnI 342 GGTACC GGTAC^C
MluNI 1793 TGGCCA TGG^CCA
MscI 1793 TGGCCA TGG^CCA
MspA1I 172 CAGCTG C[AC]G^C[GT]G
PflFI 1975 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1217 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1483 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 1684 ATTAAT AT^TAAT
PvuII 172 CAGCTG CAG^CTG
SacI 1454 GAGCTC GAGCT^C
SapI 860 GAAGAGC GCTCTTC(1/4)
Tth111I 1975 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1217 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1408 CCAACCCAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1530 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 616 CGCG CG^CG
1940 CGCG
AccIII 480 TCCGGA T^CCGGA
1877 TCCGGA
AspHI 629 GTGCTC G[AT]GC[AT]^C
1454 GAGCTC
BcgI 146 GCAAGCTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1890 GCAGGAGAATCG
BcnI 262 CCGGG CC^[GC]GG
1707 CCGGG
BseAI 480 TCCGGA T^CCGGA
1877 TCCGGA
BsiHKAI 629 GTGCTC G[AT]GC[AT]^C
1454 GAGCTC
BspEI 480 TCCGGA T^CCGGA
1877 TCCGGA
BspMI 515 ACCTGC ACCTGC(4/8)
1155 GCAGGT
BstUI 616 CGCG CG^CG
1940 CGCG
CfoI 1311 GCGC GCG^C
1941 GCGC
EaeI 1704 TGGCCG [CT]^GGCC[GA]
1793 TGGCCA
FauI 111 GCGGG CCCGC(4/6)
1756 GCGGG
HhaI 1311 GCGC GCG^C
1941 GCGC
HinP1I 1311 GCGC G^CGC
1941 GCGC
MroI 480 TCCGGA T^CCGGA
1877 TCCGGA
MseI 1685 TTAA T^TAA
1837 TTAA
MslI 328 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1846 CATAGTGGTG
MvnI 616 CGCG CG^CG
1940 CGCG
NciI 262 CCGGG CC^[GC]GG
1707 CCGGG
PstI 638 CTGCAG CTGCA^G
671 CTGCAG
SfaNI 446 GATGC GCATC(5/9)
977 GCATC
Tru9I 1685 TTAA T^TAA
1837 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BsaWI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII