################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AspEI AvrII BbeI BbrPI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI HaeII HgaI Hin1I HincII HindII HpaI KasI KspI MaeII MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 342 GGTACC G^GTACC AcsI 1025 GAATTC [GA]^AATT[CT] ApaI 15 GGGCCC GGGCC^C ApoI 1025 GAATTC [GA]^AATT[CT] AseI 1684 ATTAAT AT^TAAT AsnI 1684 ATTAAT AT^TAAT Asp700I 1530 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 342 GGTACC G^GTACC AspI 1975 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 48 CTCGGG C^[CT]CG[GA]G AviII 1310 TGCGCA TGC^GCA BalI 1793 TGGCCA TGG^CCA BamHI 477 GGATCC G^GATCC BbvCI 1884 GCTGAGG CCTCAGC(-5/-2) BciVI 1619 GTATCC GTATCC(6/5) BclI 810 TGATCA T^GATCA BglII 39 AGATCT A^GATCT BsgI 1947 CTGCAC GTGCAG(16/14) BsoBI 48 CTCGGG C^[CT]CG[GA]G BsrDI 554 GCAATG GCAATG(2/0) BsrFI 784 GCCGGT [GA]^CCGG[CT] BssSI 1766 CACGAG CACGAG(-5/-1) BstAPI 1916 GCAGAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1735 CCAGCACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 784 GCCGGT [GA]^CCGG[CT] EcoNI 354 CCTTCTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1025 GAATTC G^AATTC FbaI 810 TGATCA T^GATCA FspI 1310 TGCGCA TGC^GCA HindIII 148 AAGCTT A^AGCTT KpnI 342 GGTACC GGTAC^C MluNI 1793 TGGCCA TGG^CCA MscI 1793 TGGCCA TGG^CCA MspA1I 172 CAGCTG C[AC]G^C[GT]G PflFI 1975 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1217 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1483 GGGACCT [GA]G^G[AT]CC[CT] PshBI 1684 ATTAAT AT^TAAT PvuII 172 CAGCTG CAG^CTG SacI 1454 GAGCTC GAGCT^C SapI 860 GAAGAGC GCTCTTC(1/4) Tth111I 1975 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1217 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1408 CCAACCCAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1530 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 616 CGCG CG^CG 1940 CGCG AccIII 480 TCCGGA T^CCGGA 1877 TCCGGA AspHI 629 GTGCTC G[AT]GC[AT]^C 1454 GAGCTC BcgI 146 GCAAGCTTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1890 GCAGGAGAATCG BcnI 262 CCGGG CC^[GC]GG 1707 CCGGG BseAI 480 TCCGGA T^CCGGA 1877 TCCGGA BsiHKAI 629 GTGCTC G[AT]GC[AT]^C 1454 GAGCTC BspEI 480 TCCGGA T^CCGGA 1877 TCCGGA BspMI 515 ACCTGC ACCTGC(4/8) 1155 GCAGGT BstUI 616 CGCG CG^CG 1940 CGCG CfoI 1311 GCGC GCG^C 1941 GCGC EaeI 1704 TGGCCG [CT]^GGCC[GA] 1793 TGGCCA FauI 111 GCGGG CCCGC(4/6) 1756 GCGGG HhaI 1311 GCGC GCG^C 1941 GCGC HinP1I 1311 GCGC G^CGC 1941 GCGC MroI 480 TCCGGA T^CCGGA 1877 TCCGGA MseI 1685 TTAA T^TAA 1837 TTAA MslI 328 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1846 CATAGTGGTG MvnI 616 CGCG CG^CG 1940 CGCG NciI 262 CCGGG CC^[GC]GG 1707 CCGGG PstI 638 CTGCAG CTGCA^G 671 CTGCAG SfaNI 446 GATGC GCATC(5/9) 977 GCATC Tru9I 1685 TTAA T^TAA 1837 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII