##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
BamHI BclI BglII BsaBI BseAI
BsiWI BsmI BspDI BspEI BspHI
BsrFI BssHII Bst1107I BstAPI BstBI
BstYI BstZ17I Cfr10I ClaI CpoI
DraI DraIII Eco47III EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI KspI MamI MfeI MflI
MluI MroI MunI NaeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SspI SwaI Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 1478 GAATTT [GA]^AATT[CT]
AflII 1828 CTTAAG C^TTAAG
AflIII 1294 ACATGT A^C[GA][CT]GT
AhdI 1992 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1022 CCTCAGG CC^T[ACGT]AGG
ApoI 1478 GAATTT [GA]^AATT[CT]
AspEI 1992 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 999 CCTAGG C^CTAGG
BalI 1082 TGGCCA TGG^CCA
BbeI 1933 GGCGCC GGCGC^C
BbrPI 773 CACGTG CAC^GTG
BcgI 558 CGAGCAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1138 GTATCC GTATCC(6/5)
BfrI 1828 CTTAAG C^TTAAG
BlnI 999 CCTAGG C^CTAGG
BlpI 1716 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1716 GCTCAGC GC^T[ACGT]AGC
BsaAI 773 CACGTG [CT]AC^GT[GA]
BsaWI 519 ACCGGA [AT]^CCGG[AT]
BsgI 1225 GTGCAG GTGCAG(16/14)
BsiEI 652 CGGCCG CG[GA][CT]^CG
BspLU11I 1294 ACATGT A^CATGT
BsrDI 2190 CATTGC GCAATG(2/0)
BssSI 587 CTCGTG CACGAG(-5/-1)
BstEII 1739 GGTGACC G^GT[ACGT]ACC
BstPI 1739 GGTGACC G^GT[ACGT]ACC
Bsu36I 1022 CCTCAGG CC^T[ACGT]AGG
CelII 1716 GCTCAGC GC^T[ACGT]AGC
DsaI 744 CCATGG C^C[GA][CT]GG
EagI 652 CGGCCG C^GGCCG
Eam1105I 1992 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 652 CGGCCG C^GGCCG
Eco52I 652 CGGCCG C^GGCCG
Eco81I 1022 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1739 GGTGACC G^GT[ACGT]ACC
KasI 1933 GGCGCC G^GCGCC
MaeII 774 ACGT A^CGT
MluNI 1082 TGGCCA TGG^CCA
MscI 1082 TGGCCA TGG^CCA
NarI 1933 GGCGCC GG^CGCC
NcoI 744 CCATGG C^CATGG
NdeI 636 CATATG CA^TATG
NheI 2184 GCTAGC G^CTAGC
PmaCI 773 CACGTG CAC^GTG
PmlI 773 CACGTG CAC^GTG
SapI 2107 GCTCTTC GCTCTTC(1/4)
SbfI 59 CCTGCAGG CCTGCA^GG
SfoI 1933 GGCGCC GGC^GCC
Sse8387I 59 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 393 GTAC GT^AC
2253 GTAC
AlwI 142 GATCC GGATC(4/5)
598 GATCC
AlwNI 1154 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1232 CAGGGTCTG
AspHI 727 GAGCAC G[AT]GC[AT]^C
896 GAGCAC
AvaI 556 CCCGAG C^[CT]CG[GA]G
1662 CTCGGG
AviII 1239 TGCGCA TGC^GCA
1778 TGCGCA
BbsI 200 GTCTTC GAAGAC(2/6)
944 GTCTTC
BpuAI 200 GTCTTC GAAGAC(2/6)
944 GTCTTC
BsiHKAI 727 GAGCAC G[AT]GC[AT]^C
896 GAGCAC
BsmBI 643 CGTCTC CGTCTC(1/5)
689 GAGACG
BsoBI 556 CCCGAG C^[CT]CG[GA]G
1662 CTCGGG
Bsp1407I 392 TGTACA T^GTACA
2252 TGTACA
BspMI 58 ACCTGC ACCTGC(4/8)
348 ACCTGC
BsrBI 380 GAGCGG CCGCTC(-3/-3)
606 GAGCGG
BsrGI 392 TGTACA T^GTACA
2252 TGTACA
DrdI 269 GACGGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
869 GACGAATGTGTC
EarI 677 CTCTTC CTCTTC(1/4)
2108 CTCTTC
EcoNI 1125 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1902 CCTCTAGCAGG
FspI 1239 TGCGCA TGC^GCA
1778 TGCGCA
HaeII 11 GGCGCT [GA]GCGC^[CT]
1933 GGCGCC
HincII 242 GTCAAC GT[CT]^[GA]AC
515 GTCAAC
HindII 242 GTCAAC GT[CT]^[GA]AC
515 GTCAAC
Ksp632I 677 CTCTTC CTCTTC(1/4)
2108 CTCTTC
PstI 60 CTGCAG CTGCA^G
808 CTGCAG
RsaI 393 GTAC GT^AC
2253 GTAC
SphI 657 GCATGC GCATG^C
834 GCATGC
SspBI 392 TGTACA T^GTACA
2252 TGTACA
StuI 1311 AGGCCT AGG^CCT
1886 AGGCCT
TaqI 378 TCGA T^CGA
1990 TCGA
TfiI 1165 GAATC G^A[AT]TC
1177 GAATC
TthHB8I 378 TCGA T^CGA
1990 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AluI ApaI
AvaII BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI