################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI BamHI BclI BglII BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BsrFI BssHII Bst1107I BstAPI BstBI BstYI BstZ17I Cfr10I ClaI CpoI DraI DraIII Eco47III EcoRI EcoRV EcoT22I FbaI FseI HindIII HpaI KpnI KspI MamI MfeI MflI MluI MroI MunI NaeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 1478 GAATTT [GA]^AATT[CT] AflII 1828 CTTAAG C^TTAAG AflIII 1294 ACATGT A^C[GA][CT]GT AhdI 1992 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1022 CCTCAGG CC^T[ACGT]AGG ApoI 1478 GAATTT [GA]^AATT[CT] AspEI 1992 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 999 CCTAGG C^CTAGG BalI 1082 TGGCCA TGG^CCA BbeI 1933 GGCGCC GGCGC^C BbrPI 773 CACGTG CAC^GTG BcgI 558 CGAGCAAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1138 GTATCC GTATCC(6/5) BfrI 1828 CTTAAG C^TTAAG BlnI 999 CCTAGG C^CTAGG BlpI 1716 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1716 GCTCAGC GC^T[ACGT]AGC BsaAI 773 CACGTG [CT]AC^GT[GA] BsaWI 519 ACCGGA [AT]^CCGG[AT] BsgI 1225 GTGCAG GTGCAG(16/14) BsiEI 652 CGGCCG CG[GA][CT]^CG BspLU11I 1294 ACATGT A^CATGT BsrDI 2190 CATTGC GCAATG(2/0) BssSI 587 CTCGTG CACGAG(-5/-1) BstEII 1739 GGTGACC G^GT[ACGT]ACC BstPI 1739 GGTGACC G^GT[ACGT]ACC Bsu36I 1022 CCTCAGG CC^T[ACGT]AGG CelII 1716 GCTCAGC GC^T[ACGT]AGC DsaI 744 CCATGG C^C[GA][CT]GG EagI 652 CGGCCG C^GGCCG Eam1105I 1992 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 652 CGGCCG C^GGCCG Eco52I 652 CGGCCG C^GGCCG Eco81I 1022 CCTCAGG CC^T[ACGT]AGG EcoO65I 1739 GGTGACC G^GT[ACGT]ACC KasI 1933 GGCGCC G^GCGCC MaeII 774 ACGT A^CGT MluNI 1082 TGGCCA TGG^CCA MscI 1082 TGGCCA TGG^CCA NarI 1933 GGCGCC GG^CGCC NcoI 744 CCATGG C^CATGG NdeI 636 CATATG CA^TATG NheI 2184 GCTAGC G^CTAGC PmaCI 773 CACGTG CAC^GTG PmlI 773 CACGTG CAC^GTG SapI 2107 GCTCTTC GCTCTTC(1/4) SbfI 59 CCTGCAGG CCTGCA^GG SfoI 1933 GGCGCC GGC^GCC Sse8387I 59 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AfaI 393 GTAC GT^AC 2253 GTAC AlwI 142 GATCC GGATC(4/5) 598 GATCC AlwNI 1154 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1232 CAGGGTCTG AspHI 727 GAGCAC G[AT]GC[AT]^C 896 GAGCAC AvaI 556 CCCGAG C^[CT]CG[GA]G 1662 CTCGGG AviII 1239 TGCGCA TGC^GCA 1778 TGCGCA BbsI 200 GTCTTC GAAGAC(2/6) 944 GTCTTC BpuAI 200 GTCTTC GAAGAC(2/6) 944 GTCTTC BsiHKAI 727 GAGCAC G[AT]GC[AT]^C 896 GAGCAC BsmBI 643 CGTCTC CGTCTC(1/5) 689 GAGACG BsoBI 556 CCCGAG C^[CT]CG[GA]G 1662 CTCGGG Bsp1407I 392 TGTACA T^GTACA 2252 TGTACA BspMI 58 ACCTGC ACCTGC(4/8) 348 ACCTGC BsrBI 380 GAGCGG CCGCTC(-3/-3) 606 GAGCGG BsrGI 392 TGTACA T^GTACA 2252 TGTACA DrdI 269 GACGGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 869 GACGAATGTGTC EarI 677 CTCTTC CTCTTC(1/4) 2108 CTCTTC EcoNI 1125 CCTTGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1902 CCTCTAGCAGG FspI 1239 TGCGCA TGC^GCA 1778 TGCGCA HaeII 11 GGCGCT [GA]GCGC^[CT] 1933 GGCGCC HincII 242 GTCAAC GT[CT]^[GA]AC 515 GTCAAC HindII 242 GTCAAC GT[CT]^[GA]AC 515 GTCAAC Ksp632I 677 CTCTTC CTCTTC(1/4) 2108 CTCTTC PstI 60 CTGCAG CTGCA^G 808 CTGCAG RsaI 393 GTAC GT^AC 2253 GTAC SphI 657 GCATGC GCATG^C 834 GCATGC SspBI 392 TGTACA T^GTACA 2252 TGTACA StuI 1311 AGGCCT AGG^CCT 1886 AGGCCT TaqI 378 TCGA T^CGA 1990 TCGA TfiI 1165 GAATC G^A[AT]TC 1177 GAATC TthHB8I 378 TCGA T^CGA 1990 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcyI AluI ApaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI