##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AccII AccIII AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvrII BamHI
BbeI BbrPI BcgI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BspDI BspEI BspLU11I BspMI
BsrBI BssHII BssSI BstBI BstUI
BstYI Bsu36I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KasI KpnI KspI MamI MfeI
MflI MluI MroI MseI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
StuI SwaI TaqI TfiI Tru9I
Tth111I TthHB8I XbaI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AclI 692 AACGTT AA^CGTT
AcyI 319 GGCGTC G[GA]^CG[CT]C
AviII 73 TGCGCA TGC^GCA
BbsI 1305 GTCTTC GAAGAC(2/6)
BbvCI 1087 GCTGAGG CCTCAGC(-5/-2)
BciVI 1189 GTATCC GTATCC(6/5)
BpuAI 1305 GTCTTC GAAGAC(2/6)
BsaHI 319 GGCGTC G[GA]^CG[CT]C
BsmI 179 GCATTC GAATGC(1/-1)
Bsp1407I 731 TGTACA T^GTACA
BspHI 1380 TCATGA T^CATGA
BsrFI 260 GCCGGC [GA]^CCGG[CT]
BsrGI 731 TGTACA T^GTACA
BstAPI 1021 GCACTCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 792 GGTGACC G^GT[ACGT]ACC
BstPI 792 GGTGACC G^GT[ACGT]ACC
Cfr10I 260 GCCGGC [GA]^CCGG[CT]
DrdI 897 GACCTTCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 842 CCATGG C^C[GA][CT]GG
EcoO65I 792 GGTGACC G^GT[ACGT]ACC
FauI 1083 GCGGG CCCGC(4/6)
FspI 73 TGCGCA TGC^GCA
Hin1I 319 GGCGTC G[GA]^CG[CT]C
HincII 418 GTTGAC GT[CT]^[GA]AC
HindII 418 GTTGAC GT[CT]^[GA]AC
MslI 399 CATCGTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 260 GCCGGC GCC^GGC
NcoI 842 CCATGG C^CATGG
NgoMIV 260 GCCGGC G^CCGGC
PflMI 1353 CCATAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 692 AACGTT AA^CGTT
PstI 8 CTGCAG CTGCA^G
PvuII 881 CAGCTG CAG^CTG
RcaI 1380 TCATGA T^CATGA
SspBI 731 TGTACA T^GTACA
SspI 663 AATATT AAT^ATT
Van91I 1353 CCATAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 464 CCACTCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 757 GTATAC GT^[AC][GT]AC
822 GTATAC
AlwI 28 GGATC GGATC(4/5)
1519 GGATC
AlwNI 274 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
618 CAGTATCTG
BalI 212 TGGCCA TGG^CCA
1250 TGGCCA
BanI 432 GGTGCC G^G[CT][GA]CC
1294 GGCACC
BfaI 439 CTAG C^TAG
1405 CTAG
BlpI 121 GCTGAGC GC^T[ACGT]AGC
222 GCTGAGC
BpmI 586 CTGGAG CTGGAG(16/14)
763 CTGGAG
Bpu1102I 121 GCTGAGC GC^T[ACGT]AGC
222 GCTGAGC
BsrI 67 ACTGG ACTGG(1/-1)
617 CCAGT
Bst1107I 757 GTATAC GTA^TAC
822 GTATAC
BstXI 596 CCAACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
627 CCAGCAGAATGG
BstZ17I 757 GTATAC GTA^TAC
822 GTATAC
CelII 121 GCTGAGC GC^T[ACGT]AGC
222 GCTGAGC
DpnII 29 GATC ^GATC
1520 GATC
EaeI 212 TGGCCA [CT]^GGCC[GA]
1250 TGGCCA
EcoT14I 842 CCATGG C^C[AT][AT]GG
1210 CCTTGG
HaeII 536 GGCGCT [GA]GCGC^[CT]
981 GGCGCT
HgaI 320 GCGTC GACGC(5/10)
864 GCGTC
HinfI 196 GAGTC G^A[ACGT]TC
1545 GACTC
MaeI 439 CTAG C^TAG
1405 CTAG
MaeII 599 ACGT A^CGT
693 ACGT
MboI 29 GATC ^GATC
1520 GATC
MluNI 212 TGGCCA TGG^CCA
1250 TGGCCA
MscI 212 TGGCCA TGG^CCA
1250 TGGCCA
MspA1I 881 CAGCTG C[AC]G^C[GT]G
957 CCGCTG
NdeII 29 GATC ^GATC
1520 GATC
PleI 1545 GACTC GAGTC(4/5)
196 GAGTC
PpuMI 275 AGGTCCT [GA]G^G[AT]CC[CT]
769 GGGACCC
Sau3AI 29 GATC ^GATC
1520 GATC
SfcI 8 CTGCAG C^T[GA][CT]AG
200 CTGTAG
SmaI 168 CCCGGG CCC^GGG
798 CCCGGG
StyI 842 CCATGG C^C[AT][AT]GG
1210 CCTTGG
XmaCI 168 CCCGGG C^CCGGG
798 CCCGGG
XmaI 168 CCCGGG C^CCGGG
798 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AvaI AvaII
BanII BbvI BcnI BmyI BsaI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII EarI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
Ksp632I MaeIII MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
RsaI Sau96I ScrFI SfaNI SmlI
TseI Tsp45I Tsp509I TspRI