##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BcgI
BciVI BfrI BlnI BsaWI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BssHII BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoT22I FseI FspI HindIII
HpaI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SgrAI SnaBI
Sse8387I SwaI Tth111I XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 289 GGTACC G^GTACC
AflIII 1801 ACACGT A^C[GA][CT]GT
Asp718I 289 GGTACC G^GTACC
BbeI 148 GGCGCC GGCGC^C
BbrPI 1802 CACGTG CAC^GTG
BclI 1473 TGATCA T^GATCA
BlpI 192 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 192 GCTCAGC GC^T[ACGT]AGC
BsaAI 1802 CACGTG [CT]AC^GT[GA]
BsaBI 576 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 222 CGACCG CG[GA][CT]^CG
BstAPI 2017 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 620 TTCGAA TT^CGAA
CelII 192 GCTCAGC GC^T[ACGT]AGC
DraI 744 TTTAAA TTT^AAA
DraIII 1755 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 529 GACCTTCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 730 GATATC GAT^ATC
FbaI 1473 TGATCA T^GATCA
HincII 1419 GTCAAC GT[CT]^[GA]AC
HindII 1419 GTCAAC GT[CT]^[GA]AC
KasI 148 GGCGCC G^GCGCC
KpnI 289 GGTACC GGTAC^C
KspI 66 CCGCGG CCGC^GG
MamI 576 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 148 GGCGCC GG^CGCC
NcoI 312 CCATGG C^CATGG
NspI 905 GCATGC [GA]CATG^[CT]
NspV 620 TTCGAA TT^CGAA
PmaCI 1802 CACGTG CAC^GTG
PmlI 1802 CACGTG CAC^GTG
PvuII 725 CAGCTG CAG^CTG
SacII 66 CCGCGG CCGC^GG
SapI 1515 GCTCTTC GCTCTTC(1/4)
SfoI 148 GGCGCC GGC^GCC
SfuI 620 TTCGAA TT^CGAA
SmaI 1312 CCCGGG CCC^GGG
SpeI 2178 ACTAGT A^CTAGT
SphI 905 GCATGC GCATG^C
SspI 636 AATATT AAT^ATT
XmaCI 1312 CCCGGG C^CCGGG
XmaI 1312 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcyI 148 GGCGCC G[GA]^CG[CT]C
1596 GGCGTC
ApaI 1147 GGGCCC GGGCC^C
1320 GGGCCC
Asp700I 1000 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1174 GAATGTTTTC
AvaI 952 CCCGAG C^[CT]CG[GA]G
1312 CCCGGG
BalI 1344 TGGCCA TGG^CCA
1546 TGGCCA
BbsI 628 GAAGAC GAAGAC(2/6)
1536 GTCTTC
BbvCI 1704 CCTCAGC CCTCAGC(-5/-2)
175 GCTGAGG
BglI 257 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1588 GCCTGGTTGGC
BglII 215 AGATCT A^GATCT
551 AGATCT
BpuAI 628 GAAGAC GAAGAC(2/6)
1536 GTCTTC
BsaHI 148 GGCGCC G[GA]^CG[CT]C
1596 GGCGTC
BsgI 834 CTGCAC GTGCAG(16/14)
1667 GTGCAG
BsmI 617 GCATTC GAATGC(1/-1)
1277 GCATTC
BsoBI 952 CCCGAG C^[CT]CG[GA]G
1312 CCCGGG
Bsp1407I 1448 TGTACA T^GTACA
1994 TGTACA
BspMI 1423 ACCTGC ACCTGC(4/8)
1035 GCAGGT
BsrBI 471 CCGCTC CCGCTC(-3/-3)
477 CCGCTC
BsrGI 1448 TGTACA T^GTACA
1994 TGTACA
BstF5I 1327 CATCC GGATG(2/0)
1316 GGATG
DsaI 66 CCGCGG C^C[GA][CT]GG
312 CCATGG
EarI 1516 CTCTTC CTCTTC(1/4)
547 GAAGAG
EcoRI 1529 GAATTC G^AATTC
2049 GAATTC
FokI 1327 CATCC GGATG(9/13)
1316 GGATG
HaeII 111 GGCGCT [GA]GCGC^[CT]
148 GGCGCC
HgaI 73 GCGTC GACGC(5/10)
1597 GCGTC
Hin1I 148 GGCGCC G[GA]^CG[CT]C
1596 GGCGTC
Ksp632I 1516 CTCTTC CTCTTC(1/4)
547 GAAGAG
MluNI 1344 TGGCCA TGG^CCA
1546 TGGCCA
MscI 1344 TGGCCA TGG^CCA
1546 TGGCCA
MslI 453 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
645 CATCGAAGTG
PpuMI 965 GGGACCT [GA]G^G[AT]CC[CT]
1081 GGGACCT
PstI 1425 CTGCAG CTGCA^G
1578 CTGCAG
SacI 1060 GAGCTC GAGCT^C
1093 GAGCTC
SfaNI 2211 GATGC GCATC(5/9)
1248 GCATC
SmlI 1030 CTTGAG C^T[CT][GA]AG
1096 CTCAAG
SspBI 1448 TGTACA T^GTACA
1994 TGTACA
TfiI 1005 GATTC G^A[AT]TC
1435 GATTC
XmnI 1000 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1174 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfcI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII