##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbvCI BciVI BclI
BfrI BlnI BsaBI BsaHI BseAI
BsiEI BsiWI BsmI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI FspI HaeII Hin1I HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Alw44I 359 GTGCAC G^TGCAC
AlwNI 1404 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 359 GTGCAC G^TGCAC
AvaI 95 CTCGAG C^[CT]CG[GA]G
BalI 397 TGGCCA TGG^CCA
BbsI 1315 GTCTTC GAAGAC(2/6)
BcgI 1200 CGAGGGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1413 CCCGG CC^[GC]GG
BglI 1851 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2048 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2048 GCTTAGC GC^T[ACGT]AGC
BpuAI 1315 GTCTTC GAAGAC(2/6)
BsaAI 945 CACGTA [CT]AC^GT[GA]
BsaWI 229 ACCGGA [AT]^CCGG[AT]
BsmBI 2295 GAGACG CGTCTC(1/5)
BsoBI 95 CTCGAG C^[CT]CG[GA]G
Bsp1407I 352 TGTACA T^GTACA
BsrGI 352 TGTACA T^GTACA
CelII 2048 GCTTAGC GC^T[ACGT]AGC
EcoNI 544 CCTATGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 582 ATGCAT ATGCA^T
HgaI 2297 GACGC GACGC(5/10)
MluNI 397 TGGCCA TGG^CCA
MscI 397 TGGCCA TGG^CCA
MspA1I 1552 CAGCTG C[AC]G^C[GT]G
NciI 1413 CCCGG CC^[GC]GG
NdeI 364 CATATG CA^TATG
NheI 986 GCTAGC G^CTAGC
NsiI 582 ATGCAT ATGCA^T
PaeR7I 95 CTCGAG C^TCGAG
PflMI 1836 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1680 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1552 CAGCTG CAG^CTG
SacI 1014 GAGCTC GAGCT^C
SapI 2121 GCTCTTC GCTCTTC(1/4)
SspBI 352 TGTACA T^GTACA
Van91I 1836 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 689 TCTAGA T^CTAGA
XhoI 95 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 277 ACATGT A^C[GA][CT]GT
355 ACATGT
AocI 12 CCTGAGG CC^T[ACGT]AGG
476 CCTCAGG
Asp700I 1485 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1542 GAAACATTTC
BamHI 656 GGATCC G^GATCC
1781 GGATCC
BanI 615 GGTGCC G^G[CT][GA]CC
1832 GGTGCC
BpmI 2112 CTCCAG CTGGAG(16/14)
1987 CTGGAG
BseRI 1704 CTCCTC GAGGAG(10/8)
1191 GAGGAG
BspLU11I 277 ACATGT A^CATGT
355 ACATGT
BspMI 1604 ACCTGC ACCTGC(4/8)
1181 GCAGGT
BstXI 433 CCAATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1336 CCACAGTGGTGG
Bsu36I 12 CCTGAGG CC^T[ACGT]AGG
476 CCTCAGG
CfoI 224 GCGC GCG^C
1586 GCGC
EaeI 397 TGGCCA [CT]^GGCC[GA]
2172 CGGCCA
Eco81I 12 CCTGAGG CC^T[ACGT]AGG
476 CCTCAGG
EcoRI 1489 GAATTC G^AATTC
2094 GAATTC
HhaI 224 GCGC GCG^C
1586 GCGC
HinP1I 224 GCGC G^CGC
1586 GCGC
MfeI 661 CAATTG C^AATTG
2341 CAATTG
MunI 661 CAATTG C^AATTG
2341 CAATTG
NcoI 1478 CCATGG C^CATGG
2175 CCATGG
PpuMI 977 GGGACCT [GA]G^G[AT]CC[CT]
1934 AGGACCC
SexAI 1064 ACCAGGT A^CC[AT]GGT
1363 ACCTGGT
SfcI 319 CTGTAG C^T[GA][CT]AG
1131 CTACAG
XmnI 1485 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1542 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BfaI BglII BmyI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII