##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbrPI BcgI BciVI BfrI BglI
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmBI Bsp1407I
BspDI BspEI BspMI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
Eam1105I Eco81I EcoO65I EcoRV EcoT22I
FspI HgaI HincII HindII HpaI
KpnI MaeII MamI MluI MluNI
MroI MscI NcoI NdeI NheI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI Tth111I Van91I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 171 GGCGCC G[GA]^CG[CT]C
AfaI 466 GTAC GT^AC
AflIII 556 ACATGT A^C[GA][CT]GT
AlwI 203 GATCC GGATC(4/5)
AlwNI 1635 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1312 AGCGCT AGC^GCT
AseI 1584 ATTAAT AT^TAAT
AsnI 1584 ATTAAT AT^TAAT
Asp700I 1677 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 1363 GGGCTC G[GA]GC[CT]^C
BbeI 171 GGCGCC GGCGC^C
BbvCI 1191 GCTGAGG CCTCAGC(-5/-2)
BclI 1753 TGATCA T^GATCA
BglII 716 AGATCT A^GATCT
BlpI 1308 GCTGAGC GC^T[ACGT]AGC
BpmI 1387 CTCCAG CTGGAG(16/14)
Bpu1102I 1308 GCTGAGC GC^T[ACGT]AGC
BsaHI 171 GGCGCC G[GA]^CG[CT]C
BseRI 1194 GAGGAG GAGGAG(10/8)
BsmAI 560 GTCTC GTCTC(1/5)
BsmFI 753 GGGAC GGGAC(10/14)
BspHI 1322 TCATGA T^CATGA
BspLU11I 556 ACATGT A^CATGT
BsrBI 131 GAGCGG CCGCTC(-3/-3)
BsrDI 440 CATTGC GCAATG(2/0)
CelII 1308 GCTGAGC GC^T[ACGT]AGC
DraII 523 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1432 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 551 GACTGACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 161 CCGCGG C^C[GA][CT]GG
Eco47III 1312 AGCGCT AGC^GCT
EcoNI 527 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 523 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1107 GAATTC G^AATTC
FbaI 1753 TGATCA T^GATCA
FseI 45 GGCCGGCC GGCCGG^CC
Hin1I 171 GGCGCC G[GA]^CG[CT]C
KasI 171 GGCGCC G^GCGCC
KspI 161 CCGCGG CCGC^GG
NarI 171 GGCGCC GG^CGCC
NotI 157 GCGGCCGC GC^GGCCGC
PshBI 1584 ATTAAT AT^TAAT
PstI 1576 CTGCAG CTGCA^G
RcaI 1322 TCATGA T^CATGA
RsaI 466 GTAC GT^AC
SacII 161 CCGCGG CCGC^GG
SfoI 171 GGCGCC GGC^GCC
SphI 1556 GCATGC GCATG^C
SwaI 1204 ATTTAAAT ATTT^AAAT
Tsp45I 580 GTGAC ^GT[GC]AC
XmnI 1677 GAAACTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccII 162 CGCG CG^CG
186 CGCG
AspHI 1384 GTGCTC G[AT]GC[AT]^C
1657 GTGCTC
AvaI 76 CCCGAG C^[CT]CG[GA]G
111 CCCGAG
BbsI 1098 GAAGAC GAAGAC(2/6)
1840 GTCTTC
BfaI 1500 CTAG C^TAG
1741 CTAG
BpuAI 1098 GAAGAC GAAGAC(2/6)
1840 GTCTTC
BsiEI 44 CGGCCG CG[GA][CT]^CG
158 CGGCCG
BsiHKAI 1384 GTGCTC G[AT]GC[AT]^C
1657 GTGCTC
BsmI 373 GAATGC GAATGC(1/-1)
1690 GCATTC
BsoBI 76 CCCGAG C^[CT]CG[GA]G
111 CCCGAG
BstUI 162 CGCG CG^CG
186 CGCG
BstYI 202 AGATCC [GA]^GATC[CT]
716 AGATCT
CfoI 172 GCGC GCG^C
1313 GCGC
DraI 956 TTTAAA TTT^AAA
1205 TTTAAA
EagI 44 CGGCCG C^GGCCG
158 CGGCCG
EarI 939 GAAGAG CTCTTC(1/4)
1358 GAAGAG
EclXI 44 CGGCCG C^GGCCG
158 CGGCCG
Eco52I 44 CGGCCG C^GGCCG
158 CGGCCG
EcoT14I 260 CCTTGG C^C[AT][AT]GG
1790 CCTTGG
FauI 133 GCGGG CCCGC(4/6)
163 GCGGG
HaeII 171 GGCGCC [GA]GCGC^[CT]
1312 AGCGCT
HhaI 172 GCGC GCG^C
1313 GCGC
HinP1I 172 GCGC G^CGC
1313 GCGC
HindIII 471 AAGCTT A^AGCTT
744 AAGCTT
Ksp632I 939 GAAGAG CTCTTC(1/4)
1358 GAAGAG
MaeI 1500 CTAG C^TAG
1741 CTAG
MflI 202 AGATCC [GA]^GATC[CT]
716 AGATCT
MslI 1745 CACCATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1788 CACCTTGGTG
MspA1I 94 CAGCGG C[AC]G^C[GT]G
161 CCGCGG
MvnI 162 CGCG CG^CG
186 CGCG
PleI 1664 GACTC GAGTC(4/5)
510 GAGTC
SfcI 1531 CTACAG C^T[GA][CT]AG
1576 CTGCAG
SmlI 397 CTCAAG C^T[CT][GA]AG
1227 CTCAAG
StyI 260 CCTTGG C^C[AT][AT]GG
1790 CCTTGG
TaqI 563 TCGA T^CGA
1721 TCGA
TthHB8I 563 TCGA T^CGA
1721 TCGA
XcmI 370 CCAGAATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
874 CCAAAACAGACATGG
XhoII 202 AGATCC [GA]^GATC[CT]
716 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BanI BbvI BcnI BmyI BsaJI
BsiYI BslI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr10I Cfr13I DdeI DpnII EaeI
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MboI MboII MfeI MnlI MseI
MspI MunI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI Sau3AI Sau96I ScrFI SfaNI
SspI TfiI Tru9I TseI Tsp509I
TspRI