##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI BamHI BbrPI BbvCI BciVI
BclI BfrI BglI BglII BlpI
Bpu1102I BsaAI BsaWI BseAI BsiEI
BsiWI BsmBI BspEI BspLU11I BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoO65I
EcoRI EcoRV FbaI FseI HincII
HindII HpaI KpnI KspI MfeI
MflI MluI MroI MspA1I MunI
MvnI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
StuI SwaI Van91I XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AcyI 174 GGCGCC G[GA]^CG[CT]C
AlwI 739 GGATC GGATC(4/5)
AspHI 6 GAGCTC G[AT]GC[AT]^C
AspI 1491 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 220 TGCGCA TGC^GCA
AvrII 440 CCTAGG C^CTAGG
BanI 174 GGCGCC G^G[CT][GA]CC
BanII 6 GAGCTC G[GA]GC[CT]^C
BbeI 174 GGCGCC GGCGC^C
BcgI 703 GCAATGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 106 CCCGG CC^[GC]GG
BlnI 440 CCTAGG C^CTAGG
BpmI 231 CTGGAG CTGGAG(16/14)
BsaHI 174 GGCGCC G[GA]^CG[CT]C
BsaI 1002 GGTCTC GGTCTC(1/5)
BsiHKAI 6 GAGCTC G[AT]GC[AT]^C
BsmFI 469 GTCCC GGGAC(10/14)
BsmI 1123 GAATGC GAATGC(1/-1)
Bsp1407I 1023 TGTACA T^GTACA
BspDI 711 ATCGAT AT^CGAT
BspHI 1284 TCATGA T^CATGA
BsrBI 659 GAGCGG CCGCTC(-3/-3)
BsrGI 1023 TGTACA T^GTACA
BssSI 553 CTCGTG CACGAG(-5/-1)
ClaI 711 ATCGAT AT^CGAT
EarI 309 GAAGAG CTCTTC(1/4)
FauI 454 GCGGG CCCGC(4/6)
FspI 220 TGCGCA TGC^GCA
HapII 107 CCGG C^CGG
HgaI 210 GACGC GACGC(5/10)
Hin1I 174 GGCGCC G[GA]^CG[CT]C
HindIII 480 AAGCTT A^AGCTT
HpaII 107 CCGG C^CGG
KasI 174 GGCGCC G^GCGCC
Ksp632I 309 GAAGAG CTCTTC(1/4)
MslI 1412 CATACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 107 CCGG C^CGG
NarI 174 GGCGCC GG^CGCC
NciI 106 CCCGG CC^[GC]GG
NcoI 199 CCATGG C^CATGG
PflFI 1491 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 97 CTGCAG CTGCA^G
RcaI 1284 TCATGA T^CATGA
SacI 6 GAGCTC GAGCT^C
SfcI 97 CTGCAG C^T[GA][CT]AG
SfoI 174 GGCGCC GGC^GCC
SphI 1475 GCATGC GCATG^C
SspBI 1023 TGTACA T^GTACA
SspI 1953 AATATT AAT^ATT
Tth111I 1491 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1836 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 1024 GTAC GT^AC
1815 GTAC
AlwNI 44 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1218 CAGGCTCTG
BalI 538 TGGCCA TGG^CCA
580 TGGCCA
BbsI 22 GAAGAC GAAGAC(2/6)
207 GAAGAC
BmyI 6 GAGCTC G[AGT]GC[ACT]^C
824 GTGCCC
BpuAI 22 GAAGAC GAAGAC(2/6)
207 GAAGAC
BsaBI 578 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1931 GATAAATATC
BsgI 904 CTGCAC GTGCAG(16/14)
1523 GTGCAG
Bsp1286I 6 GAGCTC G[AGT]GC[ACT]^C
824 GTGCCC
BspMI 95 ACCTGC ACCTGC(4/8)
653 GCAGGT
DdeI 1480 CTCAG C^T[ACGT]AG
1860 CTGAG
DsaI 199 CCATGG C^C[GA][CT]GG
1200 CCACGG
EcoNI 32 CCTCGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
647 CCTAGAGCAGG
EcoT14I 199 CCATGG C^C[AT][AT]GG
440 CCTAGG
EcoT22I 1342 ATGCAT ATGCA^T
1350 ATGCAT
HaeII 46 GGCGCT [GA]GCGC^[CT]
174 GGCGCC
HphI 656 GGTGA GGTGA(8/7)
1625 TCACC
MaeII 12 ACGT A^CGT
791 ACGT
MamI 578 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1931 GATAAATATC
MluNI 538 TGGCCA TGG^CCA
580 TGGCCA
MscI 538 TGGCCA TGG^CCA
580 TGGCCA
NsiI 1342 ATGCAT ATGCA^T
1350 ATGCAT
PleI 1775 GACTC GAGTC(4/5)
270 GAGTC
RsaI 1024 GTAC GT^AC
1815 GTAC
SmlI 1055 CTTGAG C^T[CT][GA]AG
1063 CTTGAG
StyI 199 CCATGG C^C[AT][AT]GG
440 CCTAGG
TaqI 712 TCGA T^CGA
733 TCGA
TthHB8I 712 TCGA T^CGA
733 TCGA
XcmI 190 CCACCGCTACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1790 CCATTTCCCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaI
AvaII BbvI BfaI BsaJI BseRI
BsiYI BslI BsmAI BsoBI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DpnII DraI EaeI
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HinfI ItaI MaeI MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
Sau3AI Sau96I ScrFI SfaNI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI