##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvrII BbeI BbrPI
BbsI BbvCI BciVI BfrI BlnI
BpuAI BsaI BsiWI Bsp1407I BspHI
BspLU11I BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I CpoI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FseI
HincII HindII HindIII HpaI KasI
KpnI MfeI MluI MunI NarI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuII
RcaI RsrII SalI SbfI SfiI
SfoI SfuI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 115 TCCGGA T^CCGGA
Asp700I 918 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 992 TGCGCA TGC^GCA
BalI 206 TGGCCA TGG^CCA
BamHI 140 GGATCC G^GATCC
BcgI 1638 CGACATCGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 2184 CTAG C^TAG
BglI 1312 GCCTACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1584 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1584 GCTGAGC GC^T[ACGT]AGC
BsaAI 325 TACGTG [CT]AC^GT[GA]
BseAI 115 TCCGGA T^CCGGA
BsmBI 1414 GAGACG CGTCTC(1/5)
BsmI 541 GCATTC GAATGC(1/-1)
BspDI 1642 ATCGAT AT^CGAT
BspEI 115 TCCGGA T^CCGGA
BsrDI 2223 GCAATG GCAATG(2/0)
BssSI 1938 CTCGTG CACGAG(-5/-1)
CelII 1584 GCTGAGC GC^T[ACGT]AGC
ClaI 1642 ATCGAT AT^CGAT
DraI 2379 TTTAAA TTT^AAA
DraIII 922 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1020 GACCCTGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 992 TGCGCA TGC^GCA
KspI 215 CCGCGG CCGC^GG
MaeI 2184 CTAG C^TAG
MluNI 206 TGGCCA TGG^CCA
MroI 115 TCCGGA T^CCGGA
MscI 206 TGGCCA TGG^CCA
NaeI 642 GCCGGC GCC^GGC
NcoI 566 CCATGG C^CATGG
NgoMIV 642 GCCGGC G^CCGGC
NspI 2276 ACATGC [GA]CATG^[CT]
PflMI 921 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1918 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 127 GAGCTC GAGCT^C
SacII 215 CCGCGG CCGC^GG
ScaI 2246 AGTACT AGT^ACT
SgrAI 91 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1876 CTCAAG C^T[CT][GA]AG
TfiI 1194 GATTC G^A[AT]TC
Van91I 921 CCACTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 918 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 821 ACGTGT A^C[GA][CT]GT
1085 ACACGT
AlwNI 1243 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1369 CAGGACCTG
AspI 1020 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1201 GACCTGGTC
BglII 557 AGATCT A^GATCT
1481 AGATCT
BsaBI 222 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1704 GATCACCATC
BsaWI 115 TCCGGA [AT]^CCGG[AT]
2266 TCCGGT
BsmAI 497 GAGAC GTCTC(1/5)
1414 GAGAC
BsrBI 346 GAGCGG CCGCTC(-3/-3)
1587 GAGCGG
BsrI 470 ACTGG ACTGG(1/-1)
144 CCAGT
BstEII 618 GGTGACC G^GT[ACGT]ACC
1629 GGTGACC
BstPI 618 GGTGACC G^GT[ACGT]ACC
1629 GGTGACC
EarI 130 CTCTTC CTCTTC(1/4)
1851 GAAGAG
EcoO65I 618 GGTGACC G^GT[ACGT]ACC
1629 GGTGACC
Ksp632I 130 CTCTTC CTCTTC(1/4)
1851 GAAGAG
MamI 222 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1704 GATCACCATC
MslI 883 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1338 CATCCTGATG
MspA1I 215 CCGCGG C[AC]G^C[GT]G
2025 CAGCGG
PflFI 1020 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1201 GACCTGGTC
PstI 84 CTGCAG CTGCA^G
1252 CTGCAG
PvuI 221 CGATCG CGAT^CG
836 CGATCG
SapI 1851 GAAGAGC GCTCTTC(1/4)
129 GCTCTTC
SexAI 374 ACCAGGT A^CC[AT]GGT
1202 ACCTGGT
SfcI 84 CTGCAG C^T[GA][CT]AG
1252 CTGCAG
SmaI 1510 CCCGGG CCC^GGG
2053 CCCGGG
TspRI 145 CAGTG CA[GC]TG[ACGT][ACGT]^
469 CACTG
Tth111I 1020 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1201 GACCTGGTC
XmaCI 1510 CCCGGG C^CCGGG
2053 CCCGGG
XmaI 1510 CCCGGG C^CCGGG
2053 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
AvaII BanI BanII BbvI BclI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrFI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EagI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TthHB8I XcmI XhoII