##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AscI
AseI AsnI Asp718I AspEI AvrII
BbrPI BbsI BciVI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FseI HincII HindII HindIII
HpaI KpnI KspI MfeI MluI
MroI MunI NdeI NheI NruI
NsiI NspI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 842 AAATTC [GA]^AATT[CT]
AcyI 1342 GGCGCC G[GA]^CG[CT]C
Alw44I 1769 GTGCAC G^TGCAC
AlwNI 1316 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1377 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2196 AGCGCT AGC^GCT
ApaI 1446 GGGCCC GGGCC^C
ApaLI 1769 GTGCAC G^TGCAC
ApoI 842 AAATTC [GA]^AATT[CT]
Asp700I 160 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 956 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1294 GGATCC G^GATCC
BbeI 1342 GGCGCC GGCGC^C
BsaAI 1904 TACGTG [CT]AC^GT[GA]
BsaBI 2456 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1342 GGCGCC G[GA]^CG[CT]C
BsaWI 2175 TCCGGT [AT]^CCGG[AT]
BsmFI 417 GGGAC GGGAC(10/14)
BspHI 1824 TCATGA T^CATGA
BsrBI 793 CCGCTC CCGCTC(-3/-3)
BsrDI 1555 CATTGC GCAATG(2/0)
Bsu36I 1377 CCTCAGG CC^T[ACGT]AGG
DsaI 1863 CCATGG C^C[GA][CT]GG
EagI 54 CGGCCG C^GGCCG
EarI 2079 CTCTTC CTCTTC(1/4)
EclXI 54 CGGCCG C^GGCCG
Eco47III 2196 AGCGCT AGC^GCT
Eco52I 54 CGGCCG C^GGCCG
Eco81I 1377 CCTCAGG CC^T[ACGT]AGG
EcoNI 2362 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 966 GCGTC GACGC(5/10)
Hin1I 1342 GGCGCC G[GA]^CG[CT]C
KasI 1342 GGCGCC G^GCGCC
Ksp632I 2079 CTCTTC CTCTTC(1/4)
MamI 2456 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1342 GGCGCC GG^CGCC
NcoI 1863 CCATGG C^CATGG
NotI 53 GCGGCCGC GC^GGCCGC
PaeR7I 1248 CTCGAG C^TCGAG
PflFI 956 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 815 CTGCAG CTGCA^G
PvuII 1398 CAGCTG CAG^CTG
RcaI 1824 TCATGA T^CATGA
SfcI 815 CTGCAG C^T[GA][CT]AG
SfoI 1342 GGCGCC GGC^GCC
SmlI 1248 CTCGAG C^T[CT][GA]AG
Tth111I 956 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1248 CTCGAG C^TCGAG
XmnI 160 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AviII 468 TGCGCA TGC^GCA
993 TGCGCA
BalI 687 TGGCCA TGG^CCA
1305 TGGCCA
BbvCI 1120 GCTGAGG CCTCAGC(-5/-2)
1502 GCTGAGG
BcgI 59 GCACGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
406 GCAGCGGAGTCG
BclI 759 TGATCA T^GATCA
874 TGATCA
BsgI 1677 CTGCAC GTGCAG(16/14)
2420 CTGCAC
BsmI 280 GAATGC GAATGC(1/-1)
1569 GCATTC
BspMI 1672 ACCTGC ACCTGC(4/8)
2272 GCAGGT
BstXI 675 CCAAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1989 CCACTGCTCTGG
BstYI 521 GGATCT [GA]^GATC[CT]
1294 GGATCC
FbaI 759 TGATCA T^GATCA
874 TGATCA
FspI 468 TGCGCA TGC^GCA
993 TGCGCA
HphI 1107 TCACC GGTGA(8/7)
1575 TCACC
MflI 521 GGATCT [GA]^GATC[CT]
1294 GGATCC
MluNI 687 TGGCCA TGG^CCA
1305 TGGCCA
MscI 687 TGGCCA TGG^CCA
1305 TGGCCA
MseI 114 TTAA T^TAA
2489 TTAA
NaeI 1565 GCCGGC GCC^GGC
1782 GCCGGC
NgoMIV 1565 GCCGGC G^CCGGC
1782 GCCGGC
PpuMI 417 GGGACCC [GA]G^G[AT]CC[CT]
2334 GGGTCCT
SacI 1103 GAGCTC GAGCT^C
2258 GAGCTC
Tru9I 114 TTAA T^TAA
2489 TTAA
XcmI 675 CCAAGATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2049 CCATCAGGCTAATGG
XhoII 521 GGATCT [GA]^GATC[CT]
1294 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaI BsaJI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsoBI Bsp1286I
BsrFI BsrI BssKI BssSI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I