################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AscI AseI AsnI Asp718I AspEI AvrII BbrPI BbsI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MroI MunI NdeI NheI NruI NsiI NspI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 842 AAATTC [GA]^AATT[CT] AcyI 1342 GGCGCC G[GA]^CG[CT]C Alw44I 1769 GTGCAC G^TGCAC AlwNI 1316 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1377 CCTCAGG CC^T[ACGT]AGG Aor51HI 2196 AGCGCT AGC^GCT ApaI 1446 GGGCCC GGGCC^C ApaLI 1769 GTGCAC G^TGCAC ApoI 842 AAATTC [GA]^AATT[CT] Asp700I 160 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 956 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1294 GGATCC G^GATCC BbeI 1342 GGCGCC GGCGC^C BsaAI 1904 TACGTG [CT]AC^GT[GA] BsaBI 2456 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1342 GGCGCC G[GA]^CG[CT]C BsaWI 2175 TCCGGT [AT]^CCGG[AT] BsmFI 417 GGGAC GGGAC(10/14) BspHI 1824 TCATGA T^CATGA BsrBI 793 CCGCTC CCGCTC(-3/-3) BsrDI 1555 CATTGC GCAATG(2/0) Bsu36I 1377 CCTCAGG CC^T[ACGT]AGG DsaI 1863 CCATGG C^C[GA][CT]GG EagI 54 CGGCCG C^GGCCG EarI 2079 CTCTTC CTCTTC(1/4) EclXI 54 CGGCCG C^GGCCG Eco47III 2196 AGCGCT AGC^GCT Eco52I 54 CGGCCG C^GGCCG Eco81I 1377 CCTCAGG CC^T[ACGT]AGG EcoNI 2362 CCTTGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 966 GCGTC GACGC(5/10) Hin1I 1342 GGCGCC G[GA]^CG[CT]C KasI 1342 GGCGCC G^GCGCC Ksp632I 2079 CTCTTC CTCTTC(1/4) MamI 2456 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1342 GGCGCC GG^CGCC NcoI 1863 CCATGG C^CATGG NotI 53 GCGGCCGC GC^GGCCGC PaeR7I 1248 CTCGAG C^TCGAG PflFI 956 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 815 CTGCAG CTGCA^G PvuII 1398 CAGCTG CAG^CTG RcaI 1824 TCATGA T^CATGA SfcI 815 CTGCAG C^T[GA][CT]AG SfoI 1342 GGCGCC GGC^GCC SmlI 1248 CTCGAG C^T[CT][GA]AG Tth111I 956 GACCACGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1248 CTCGAG C^TCGAG XmnI 160 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AviII 468 TGCGCA TGC^GCA 993 TGCGCA BalI 687 TGGCCA TGG^CCA 1305 TGGCCA BbvCI 1120 GCTGAGG CCTCAGC(-5/-2) 1502 GCTGAGG BcgI 59 GCACGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 406 GCAGCGGAGTCG BclI 759 TGATCA T^GATCA 874 TGATCA BsgI 1677 CTGCAC GTGCAG(16/14) 2420 CTGCAC BsmI 280 GAATGC GAATGC(1/-1) 1569 GCATTC BspMI 1672 ACCTGC ACCTGC(4/8) 2272 GCAGGT BstXI 675 CCAAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1989 CCACTGCTCTGG BstYI 521 GGATCT [GA]^GATC[CT] 1294 GGATCC FbaI 759 TGATCA T^GATCA 874 TGATCA FspI 468 TGCGCA TGC^GCA 993 TGCGCA HphI 1107 TCACC GGTGA(8/7) 1575 TCACC MflI 521 GGATCT [GA]^GATC[CT] 1294 GGATCC MluNI 687 TGGCCA TGG^CCA 1305 TGGCCA MscI 687 TGGCCA TGG^CCA 1305 TGGCCA MseI 114 TTAA T^TAA 2489 TTAA NaeI 1565 GCCGGC GCC^GGC 1782 GCCGGC NgoMIV 1565 GCCGGC G^CCGGC 1782 GCCGGC PpuMI 417 GGGACCC [GA]G^G[AT]CC[CT] 2334 GGGTCCT SacI 1103 GAGCTC GAGCT^C 2258 GAGCTC Tru9I 114 TTAA T^TAA 2489 TTAA XcmI 675 CCAAGATGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2049 CCATCAGGCTAATGG XhoII 521 GGATCT [GA]^GATC[CT] 1294 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I