##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AclI AflIII AgeI AhdI
Alw44I ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbeI BbsI
BciVI BglI BlnI BpuAI BsaBI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssSI BstBI BstEII BstPI BstXI
ClaI CpoI DraIII DrdI Eam1105I
EcoO65I EcoRI EcoT14I FseI FspI
HpaI KasI KpnI MamI MfeI
MluI MluNI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI StuI StyI
Tth111I Van91I XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 641 GACGTC GACGT^C
AccI 899 GTATAC GT^[AC][GT]AC
AccIII 39 TCCGGA T^CCGGA
AcyI 641 GACGTC G[GA]^CG[CT]C
AflII 1574 CTTAAG C^TTAAG
AlwNI 599 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1799 CCTCAGG CC^T[ACGT]AGG
Aor51HI 400 AGCGCT AGC^GCT
ApaI 199 GGGCCC GGGCC^C
AvaI 203 CCCGAG C^[CT]CG[GA]G
BanII 199 GGGCCC G[GA]GC[CT]^C
BbrPI 755 CACGTG CAC^GTG
BbvCI 316 GCTGAGG CCTCAGC(-5/-2)
BclI 700 TGATCA T^GATCA
BfrI 1574 CTTAAG C^TTAAG
BglII 1283 AGATCT A^GATCT
BmyI 199 GGGCCC G[AGT]GC[ACT]^C
BsaAI 755 CACGTG [CT]AC^GT[GA]
BsaHI 641 GACGTC G[GA]^CG[CT]C
BsaWI 39 TCCGGA [AT]^CCGG[AT]
BseAI 39 TCCGGA T^CCGGA
BseRI 35 CTCCTC GAGGAG(10/8)
BsiEI 535 CGGCCG CG[GA][CT]^CG
BsoBI 203 CCCGAG C^[CT]CG[GA]G
Bsp1286I 199 GGGCCC G[AGT]GC[ACT]^C
BspEI 39 TCCGGA T^CCGGA
BsrFI 377 ACCGGC [GA]^CCGG[CT]
Bst1107I 899 GTATAC GTA^TAC
BstZ17I 899 GTATAC GTA^TAC
Bsu36I 1799 CCTCAGG CC^T[ACGT]AGG
Cfr10I 377 ACCGGC [GA]^CCGG[CT]
EagI 535 CGGCCG C^GGCCG
EclXI 535 CGGCCG C^GGCCG
Eco47III 400 AGCGCT AGC^GCT
Eco52I 535 CGGCCG C^GGCCG
Eco81I 1799 CCTCAGG CC^T[ACGT]AGG
EcoNI 1799 CCTCAGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 939 GATATC GAT^ATC
EcoT22I 1110 ATGCAT ATGCA^T
FbaI 700 TGATCA T^GATCA
HgaI 490 GCGTC GACGC(5/10)
Hin1I 641 GACGTC G[GA]^CG[CT]C
HincII 179 GTCAAC GT[CT]^[GA]AC
HindII 179 GTCAAC GT[CT]^[GA]AC
HindIII 1466 AAGCTT A^AGCTT
HphI 552 TCACC GGTGA(8/7)
MroI 39 TCCGGA T^CCGGA
MslI 247 CACCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 1110 ATGCAT ATGCA^T
NspI 1428 ACATGC [GA]CATG^[CT]
PmaCI 755 CACGTG CAC^GTG
PmlI 755 CACGTG CAC^GTG
SapI 502 GCTCTTC GCTCTTC(1/4)
SbfI 595 CCTGCAGG CCTGCA^GG
Sse8387I 595 CCTGCAGG CCTGCA^GG
Tsp45I 2141 GTCAC ^GT[GC]AC
XbaI 109 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 307 GATCC GGATC(4/5)
916 GATCC
Asp700I 1633 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2100 GAAGTTTTTC
BanI 155 GGCACC G^G[CT][GA]CC
281 GGCACC
BcgI 388 CGACCTGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
915 CGATCCAGCTGC
BlpI 139 GCTCAGC GC^T[ACGT]AGC
2119 GCTTAGC
Bpu1102I 139 GCTCAGC GC^T[ACGT]AGC
2119 GCTTAGC
BsaI 982 GGTCTC GGTCTC(1/5)
1131 GGTCTC
BspMI 242 ACCTGC ACCTGC(4/8)
845 GCAGGT
BsrDI 2115 CATTGC GCAATG(2/0)
1415 GCAATG
BssHII 21 GCGCGC G^CGCGC
95 GCGCGC
BstAPI 328 GCACGGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
466 GCAGGATATGC
BstYI 306 AGATCC [GA]^GATC[CT]
1283 AGATCT
CelII 139 GCTCAGC GC^T[ACGT]AGC
2119 GCTTAGC
EaeI 405 TGGCCG [CT]^GGCC[GA]
535 CGGCCG
KspI 60 CCGCGG CCGC^GG
346 CCGCGG
MaeII 642 ACGT A^CGT
756 ACGT
MflI 306 AGATCC [GA]^GATC[CT]
1283 AGATCT
SacII 60 CCGCGG CCGC^GG
346 CCGCGG
SfaNI 13 GATGC GCATC(5/9)
1112 GCATC
SmlI 1480 CTTGAG C^T[CT][GA]AG
1574 CTTAAG
SwaI 837 ATTTAAAT ATTT^AAAT
859 ATTTAAAT
TfiI 1409 GAATC G^A[AT]TC
2095 GAATC
XhoII 306 AGATCC [GA]^GATC[CT]
1283 AGATCT
XmnI 1633 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2100 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaII BbvI BcnI BfaI
BpmI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfcI SspI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I