##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BbeI
BbrPI BbvCI BcgI BciVI BglII
BlnI BpmI BsaAI BsaBI BsaHI
BseAI BseRI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RsrII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AclI 970 AACGTT AA^CGTT
AcsI 652 AAATTT [GA]^AATT[CT]
AflII 166 CTTAAG C^TTAAG
AflIII 1265 ACGTGT A^C[GA][CT]GT
Aor51HI 1223 AGCGCT AGC^GCT
ApaI 110 GGGCCC GGGCC^C
ApoI 652 AAATTT [GA]^AATT[CT]
Asp700I 376 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 339 GGATCC G^GATCC
BanI 739 GGCACC G^G[CT][GA]CC
BclI 287 TGATCA T^GATCA
BcnI 23 CCCGG CC^[GC]GG
BfrI 166 CTTAAG C^TTAAG
BglI 1385 GCCGCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 1367 ACCGGA [AT]^CCGG[AT]
BsmBI 1043 GAGACG CGTCTC(1/5)
BspHI 830 TCATGA T^CATGA
BstAPI 4 GCAGAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1254 GGTGACC G^GT[ACGT]ACC
BstPI 1254 GGTGACC G^GT[ACGT]ACC
BstYI 339 GGATCC [GA]^GATC[CT]
DraIII 1165 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1223 AGCGCT AGC^GCT
EcoO65I 1254 GGTGACC G^GT[ACGT]ACC
FbaI 287 TGATCA T^GATCA
HaeII 1223 AGCGCT [GA]GCGC^[CT]
HgaI 71 GCGTC GACGC(5/10)
KspI 1275 CCGCGG CCGC^GG
MflI 339 GGATCC [GA]^GATC[CT]
MslI 1260 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 23 CCCGG CC^[GC]GG
NheI 672 GCTAGC G^CTAGC
Psp1406I 970 AACGTT AA^CGTT
PvuII 527 CAGCTG CAG^CTG
RcaI 830 TCATGA T^CATGA
SacII 1275 CCGCGG CCGC^GG
SapI 376 GAAGAGC GCTCTTC(1/4)
ScaI 1019 AGTACT AGT^ACT
SexAI 1166 ACCAGGT A^CC[AT]GGT
StuI 1058 AGGCCT AGG^CCT
Tsp45I 1255 GTGAC ^GT[GC]AC
XcmI 501 CCAATCTCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 339 GGATCC [GA]^GATC[CT]
XmnI 376 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AvaII 89 GGACC G^G[AT]CC
957 GGTCC
BalI 95 TGGCCA TGG^CCA
542 TGGCCA
BbsI 231 GAAGAC GAAGAC(2/6)
925 GAAGAC
BfaI 673 CTAG C^TAG
1130 CTAG
BlpI 475 GCTGAGC GC^T[ACGT]AGC
549 GCTTAGC
Bpu1102I 475 GCTGAGC GC^T[ACGT]AGC
549 GCTTAGC
BpuAI 231 GAAGAC GAAGAC(2/6)
925 GAAGAC
BsaI 997 GAGACC GGTCTC(1/5)
1354 GAGACC
BspMI 564 ACCTGC ACCTGC(4/8)
1361 ACCTGC
BsrDI 458 CATTGC GCAATG(2/0)
1089 CATTGC
BssSI 66 CACGAG CACGAG(-5/-1)
40 CTCGTG
CelII 475 GCTGAGC GC^T[ACGT]AGC
549 GCTTAGC
EaeI 95 TGGCCA [CT]^GGCC[GA]
542 TGGCCA
HapII 24 CCGG C^CGG
1368 CCGG
HpaII 24 CCGG C^CGG
1368 CCGG
MaeI 673 CTAG C^TAG
1130 CTAG
MaeIII 1067 GTAAC ^GT[ACGT]AC
1255 GTGAC
MluNI 95 TGGCCA TGG^CCA
542 TGGCCA
MscI 95 TGGCCA TGG^CCA
542 TGGCCA
MspI 24 CCGG C^CGG
1368 CCGG
NcoI 92 CCATGG C^CATGG
419 CCATGG
PflMI 948 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1164 CCACCAGGTGG
PstI 331 CTGCAG CTGCA^G
1211 CTGCAG
SacI 610 GAGCTC GAGCT^C
1147 GAGCTC
SfcI 331 CTGCAG C^T[GA][CT]AG
1211 CTGCAG
TaqI 53 TCGA T^CGA
687 TCGA
TthHB8I 53 TCGA T^CGA
687 TCGA
Van91I 948 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1164 CCACCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI BanII BbvI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeII
MboI MboII MnlI MseI MspA1I
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SmlI StyI TfiI
Tru9I TseI Tsp509I TspRI