##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvrII BcgI BciVI
BfrI BlnI BlpI Bpu1102I BsiWI
BsmI BspDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoT22I
FseI HincII HindII HindIII HpaI
KpnI KspI MluI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 1876 GACGTC GACGT^C
AccI 1654 GTCTAC GT^[AC][GT]AC
AccIII 2478 TCCGGA T^CCGGA
AspI 2182 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 554 TGCGCA TGC^GCA
BamHI 1389 GGATCC G^GATCC
BbrPI 828 CACGTG CAC^GTG
BclI 692 TGATCA T^GATCA
BglI 2001 GCCCCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1442 AGATCT A^GATCT
BsaBI 2346 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2478 TCCGGA T^CCGGA
BsgI 229 GTGCAG GTGCAG(16/14)
BsmBI 2604 CGTCTC CGTCTC(1/5)
Bsp1407I 2486 TGTACA T^GTACA
BspEI 2478 TCCGGA T^CCGGA
BspMI 1658 ACCTGC ACCTGC(4/8)
BsrGI 2486 TGTACA T^GTACA
BstAPI 824 GCAACACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2315 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 2596 GACAACAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2696 CGGCCG C^GGCCG
EclXI 2696 CGGCCG C^GGCCG
Eco52I 2696 CGGCCG C^GGCCG
EcoRV 1582 GATATC GAT^ATC
FbaI 692 TGATCA T^GATCA
FspI 554 TGCGCA TGC^GCA
MamI 2346 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1977 CAATTG C^AATTG
MroI 2478 TCCGGA T^CCGGA
MunI 1977 CAATTG C^AATTG
NcoI 1385 CCATGG C^CATGG
PflFI 2182 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 828 CACGTG CAC^GTG
PmlI 828 CACGTG CAC^GTG
SapI 2238 GCTCTTC GCTCTTC(1/4)
ScaI 884 AGTACT AGT^ACT
SmaI 1745 CCCGGG CCC^GGG
SphI 2528 GCATGC GCATG^C
SspBI 2486 TGTACA T^GTACA
Tth111I 2182 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 393 TCTAGA T^CTAGA
XcmI 355 CCAGCAGCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1745 CCCGGG C^CCGGG
XmaI 1745 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
ApaI 1885 GGGCCC GGGCC^C
1933 GGGCCC
AspHI 913 GAGCTC G[AT]GC[AT]^C
2236 GAGCTC
BbvCI 858 CCTCAGC CCTCAGC(-5/-2)
2161 GCTGAGG
BsaWI 1179 ACCGGA [AT]^CCGG[AT]
2478 TCCGGA
BsiHKAI 913 GAGCTC G[AT]GC[AT]^C
2236 GAGCTC
BspHI 749 TCATGA T^CATGA
836 TCATGA
BspLU11I 761 ACATGT A^CATGT
1017 ACATGT
BsrBI 1002 CCGCTC CCGCTC(-3/-3)
568 GAGCGG
DsaI 1254 CCACGG C^C[GA][CT]GG
1385 CCATGG
EcoNI 312 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2259 CCTGGAGAAGG
MseI 1127 TTAA T^TAA
2765 TTAA
NaeI 172 GCCGGC GCC^GGC
2698 GCCGGC
NgoMIV 172 GCCGGC G^CCGGC
2698 GCCGGC
PflMI 1053 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2667 CCAGGACGTGG
RcaI 749 TCATGA T^CATGA
836 TCATGA
SacI 913 GAGCTC GAGCT^C
2236 GAGCTC
SexAI 374 ACCTGGT A^CC[AT]GGT
971 ACCTGGT
StuI 108 AGGCCT AGG^CCT
959 AGGCCT
Tru9I 1127 TTAA T^TAA
2765 TTAA
Van91I 1053 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2667 CCAGGACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII