##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI AocI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BglII BlnI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FbaI FseI FspI Hin1I HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 1139 GTCTAC GT^[AC][GT]AC
AflII 1111 CTTAAG C^TTAAG
Alw44I 1394 GTGCAC G^TGCAC
ApaI 1343 GGGCCC GGGCC^C
ApaLI 1394 GTGCAC G^TGCAC
AspI 221 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 35 CCCGGG C^[CT]CG[GA]G
BamHI 143 GGATCC G^GATCC
BbsI 1106 GTCTTC GAAGAC(2/6)
BfrI 1111 CTTAAG C^TTAAG
BglI 124 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 622 GCTTAGC GC^T[ACGT]AGC
BpmI 239 CTCCAG CTGGAG(16/14)
Bpu1102I 622 GCTTAGC GC^T[ACGT]AGC
BpuAI 1106 GTCTTC GAAGAC(2/6)
BsaI 1764 GGTCTC GGTCTC(1/5)
BsmI 637 GAATGC GAATGC(1/-1)
BsoBI 35 CCCGGG C^[CT]CG[GA]G
BspHI 150 TCATGA T^CATGA
BsrDI 974 GCAATG GCAATG(2/0)
BstEII 530 GGTGACC G^GT[ACGT]ACC
BstPI 530 GGTGACC G^GT[ACGT]ACC
BstYI 143 GGATCC [GA]^GATC[CT]
CelII 622 GCTTAGC GC^T[ACGT]AGC
DpnII 144 GATC ^GATC
EcoNI 766 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 530 GGTGACC G^GT[ACGT]ACC
EcoRI 569 GAATTC G^AATTC
HgaI 334 GCGTC GACGC(5/10)
MboI 144 GATC ^GATC
MflI 143 GGATCC [GA]^GATC[CT]
NdeI 833 CATATG CA^TATG
NdeII 144 GATC ^GATC
PflFI 221 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1423 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 815 CAGCTG CAG^CTG
RcaI 150 TCATGA T^CATGA
Sau3AI 144 GATC ^GATC
SmaI 35 CCCGGG CCC^GGG
SmlI 1111 CTTAAG C^T[CT][GA]AG
SphI 40 GCATGC GCATG^C
Tth111I 221 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1423 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 143 GGATCC [GA]^GATC[CT]
XmaCI 35 CCCGGG C^CCGGG
XmaI 35 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 285 GTAC GT^AC
856 GTAC
AlwI 144 GATCC GGATC(4/5)
143 GGATC
AlwNI 997 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1500 CAGTCCCTG
BanII 372 GAGCCC G[GA]GC[CT]^C
1343 GGGCCC
BsaAI 408 CACGTA [CT]AC^GT[GA]
1397 CACGTA
BseRI 470 CTCCTC GAGGAG(10/8)
326 GAGGAG
BspMI 1143 ACCTGC ACCTGC(4/8)
1226 GCAGGT
BsrBI 1331 CCGCTC CCGCTC(-3/-3)
386 GAGCGG
DsaI 97 CCGCGG C^C[GA][CT]GG
125 CCGCGG
HaeII 77 AGCGCC [GA]GCGC^[CT]
1040 AGCGCC
KspI 97 CCGCGG CCGC^GG
125 CCGCGG
NspI 40 GCATGC [GA]CATG^[CT]
732 ACATGC
PpuMI 243 AGGACCT [GA]G^G[AT]CC[CT]
1326 AGGACCC
RsaI 285 GTAC GT^AC
856 GTAC
SacII 97 CCGCGG CCGC^GG
125 CCGCGG
SapI 369 GAAGAGC GCTCTTC(1/4)
736 GCTCTTC
SfaNI 302 GATGC GCATC(5/9)
1195 GCATC
TfiI 258 GAATC G^A[AT]TC
309 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
AspHI AvaII BbvI BcnI BfaI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PleI
PstI Sau96I ScrFI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I