################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BglII BlnI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 1139 GTCTAC GT^[AC][GT]AC AflII 1111 CTTAAG C^TTAAG Alw44I 1394 GTGCAC G^TGCAC ApaI 1343 GGGCCC GGGCC^C ApaLI 1394 GTGCAC G^TGCAC AspI 221 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 35 CCCGGG C^[CT]CG[GA]G BamHI 143 GGATCC G^GATCC BbsI 1106 GTCTTC GAAGAC(2/6) BfrI 1111 CTTAAG C^TTAAG BglI 124 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 622 GCTTAGC GC^T[ACGT]AGC BpmI 239 CTCCAG CTGGAG(16/14) Bpu1102I 622 GCTTAGC GC^T[ACGT]AGC BpuAI 1106 GTCTTC GAAGAC(2/6) BsaI 1764 GGTCTC GGTCTC(1/5) BsmI 637 GAATGC GAATGC(1/-1) BsoBI 35 CCCGGG C^[CT]CG[GA]G BspHI 150 TCATGA T^CATGA BsrDI 974 GCAATG GCAATG(2/0) BstEII 530 GGTGACC G^GT[ACGT]ACC BstPI 530 GGTGACC G^GT[ACGT]ACC BstYI 143 GGATCC [GA]^GATC[CT] CelII 622 GCTTAGC GC^T[ACGT]AGC DpnII 144 GATC ^GATC EcoNI 766 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 530 GGTGACC G^GT[ACGT]ACC EcoRI 569 GAATTC G^AATTC HgaI 334 GCGTC GACGC(5/10) MboI 144 GATC ^GATC MflI 143 GGATCC [GA]^GATC[CT] NdeI 833 CATATG CA^TATG NdeII 144 GATC ^GATC PflFI 221 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1423 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 815 CAGCTG CAG^CTG RcaI 150 TCATGA T^CATGA Sau3AI 144 GATC ^GATC SmaI 35 CCCGGG CCC^GGG SmlI 1111 CTTAAG C^T[CT][GA]AG SphI 40 GCATGC GCATG^C Tth111I 221 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1423 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 143 GGATCC [GA]^GATC[CT] XmaCI 35 CCCGGG C^CCGGG XmaI 35 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 285 GTAC GT^AC 856 GTAC AlwI 144 GATCC GGATC(4/5) 143 GGATC AlwNI 997 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG 1500 CAGTCCCTG BanII 372 GAGCCC G[GA]GC[CT]^C 1343 GGGCCC BsaAI 408 CACGTA [CT]AC^GT[GA] 1397 CACGTA BseRI 470 CTCCTC GAGGAG(10/8) 326 GAGGAG BspMI 1143 ACCTGC ACCTGC(4/8) 1226 GCAGGT BsrBI 1331 CCGCTC CCGCTC(-3/-3) 386 GAGCGG DsaI 97 CCGCGG C^C[GA][CT]GG 125 CCGCGG HaeII 77 AGCGCC [GA]GCGC^[CT] 1040 AGCGCC KspI 97 CCGCGG CCGC^GG 125 CCGCGG NspI 40 GCATGC [GA]CATG^[CT] 732 ACATGC PpuMI 243 AGGACCT [GA]G^G[AT]CC[CT] 1326 AGGACCC RsaI 285 GTAC GT^AC 856 GTAC SacII 97 CCGCGG CCGC^GG 125 CCGCGG SapI 369 GAAGAGC GCTCTTC(1/4) 736 GCTCTTC SfaNI 302 GATGC GCATC(5/9) 1195 GCATC TfiI 258 GAATC G^A[AT]TC 309 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 72 enzymes) # ######################################### AccII AciI AcsI AluI ApoI AspHI AvaII BbvI BcnI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI Sau96I ScrFI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I