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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspI AvaI
BanI BbeI BbsI BbvCI BcgI
BciVI BfrI BglI BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstUI BstXI BstZ17I Cfr10I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV FseI
HaeII HgaI Hin1I KasI KpnI
KspI MamI MluI MroI MspA1I
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
Psp1406I PvuI PvuII RsrII SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AocI 51 CCTCAGG CC^T[ACGT]AGG
AseI 1680 ATTAAT AT^TAAT
AsnI 1680 ATTAAT AT^TAAT
AspHI 185 GAGCTC G[AT]GC[AT]^C
AvaII 1251 GGACC G^G[AT]CC
AviII 1225 TGCGCA TGC^GCA
AvrII 1994 CCTAGG C^CTAGG
BalI 532 TGGCCA TGG^CCA
BamHI 263 GGATCC G^GATCC
BbrPI 653 CACGTG CAC^GTG
BcnI 816 CCGGG CC^[GC]GG
BglII 108 AGATCT A^GATCT
BlnI 1994 CCTAGG C^CTAGG
BsaAI 653 CACGTG [CT]AC^GT[GA]
BsiHKAI 185 GAGCTC G[AT]GC[AT]^C
BspHI 1267 TCATGA T^CATGA
BspLU11I 1705 ACATGT A^CATGT
BspMI 443 GCAGGT ACCTGC(4/8)
BsrBI 1017 GAGCGG CCGCTC(-3/-3)
BstBI 1003 TTCGAA TT^CGAA
BstEII 838 GGTGACC G^GT[ACGT]ACC
BstPI 838 GGTGACC G^GT[ACGT]ACC
Bsu36I 51 CCTCAGG CC^T[ACGT]AGG
EaeI 532 TGGCCA [CT]^GGCC[GA]
Eco81I 51 CCTCAGG CC^T[ACGT]AGG
EcoNI 722 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 838 GGTGACC G^GT[ACGT]ACC
EcoT22I 393 ATGCAT ATGCA^T
FauI 1019 GCGGG CCCGC(4/6)
FspI 1225 TGCGCA TGC^GCA
HapII 816 CCGG C^CGG
HindIII 2061 AAGCTT A^AGCTT
HpaI 1927 GTTAAC GTT^AAC
HpaII 816 CCGG C^CGG
MfeI 480 CAATTG C^AATTG
MluNI 532 TGGCCA TGG^CCA
MscI 532 TGGCCA TGG^CCA
MslI 1812 CATAGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 816 CCGG C^CGG
MunI 480 CAATTG C^AATTG
NciI 816 CCGGG CC^[GC]GG
NcoI 919 CCATGG C^CATGG
NsiI 393 ATGCAT ATGCA^T
NspI 1705 ACATGT [GA]CATG^[CT]
NspV 1003 TTCGAA TT^CGAA
PflMI 479 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 653 CACGTG CAC^GTG
PmlI 653 CACGTG CAC^GTG
PshBI 1680 ATTAAT AT^TAAT
RcaI 1267 TCATGA T^CATGA
SacI 185 GAGCTC GAGCT^C
SapI 1574 GCTCTTC GCTCTTC(1/4)
ScaI 1911 AGTACT AGT^ACT
SfuI 1003 TTCGAA TT^CGAA
SmlI 1066 CTCAAG C^T[CT][GA]AG
Van91I 479 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1430 CCAGTGCCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1705 ACATGT A^C[GA][CT]GT
1923 ACACGT
Asp700I 1571 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1985 GAAAAGTTTC
BanII 185 GAGCTC G[GA]GC[CT]^C
862 GAGCCC
BclI 801 TGATCA T^GATCA
1294 TGATCA
BfaI 1802 CTAG C^TAG
1995 CTAG
BlpI 5 GCTGAGC GC^T[ACGT]AGC
1585 GCTAAGC
BpmI 690 CTGGAG CTGGAG(16/14)
845 CTGGAG
Bpu1102I 5 GCTGAGC GC^T[ACGT]AGC
1585 GCTAAGC
BsrDI 429 CATTGC GCAATG(2/0)
1229 CATTGC
CelII 5 GCTGAGC GC^T[ACGT]AGC
1585 GCTAAGC
CfoI 433 GCGC GCG^C
1226 GCGC
DraII 583 AGGGCCT [GA]G^G[ACGT]CC[CT]
1070 AGGCCCT
EcoO109I 583 AGGGCCT [GA]G^G[ACGT]CC[CT]
1070 AGGCCCT
FbaI 801 TGATCA T^GATCA
1294 TGATCA
HhaI 433 GCGC GCG^C
1226 GCGC
HinP1I 433 GCGC G^CGC
1226 GCGC
HincII 221 GTTGAC GT[CT]^[GA]AC
1927 GTTAAC
HindII 221 GTTGAC GT[CT]^[GA]AC
1927 GTTAAC
MaeI 1802 CTAG C^TAG
1995 CTAG
PstI 1383 CTGCAG CTGCA^G
1932 CTGCAG
SspI 701 AATATT AAT^ATT
2036 AATATT
TaqI 330 TCGA T^CGA
1004 TCGA
TthHB8I 330 TCGA T^CGA
1004 TCGA
XmnI 1571 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1985 GAAAAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BmyI BsaJI BseRI
BsgI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DrdI
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII