################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AvaI BanI BbeI BbsI BbvCI BcgI BciVI BfrI BglI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FseI HaeII HgaI Hin1I KasI KpnI KspI MamI MluI MroI MspA1I MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI Psp1406I PvuI PvuII RsrII SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AocI 51 CCTCAGG CC^T[ACGT]AGG AseI 1680 ATTAAT AT^TAAT AsnI 1680 ATTAAT AT^TAAT AspHI 185 GAGCTC G[AT]GC[AT]^C AvaII 1251 GGACC G^G[AT]CC AviII 1225 TGCGCA TGC^GCA AvrII 1994 CCTAGG C^CTAGG BalI 532 TGGCCA TGG^CCA BamHI 263 GGATCC G^GATCC BbrPI 653 CACGTG CAC^GTG BcnI 816 CCGGG CC^[GC]GG BglII 108 AGATCT A^GATCT BlnI 1994 CCTAGG C^CTAGG BsaAI 653 CACGTG [CT]AC^GT[GA] BsiHKAI 185 GAGCTC G[AT]GC[AT]^C BspHI 1267 TCATGA T^CATGA BspLU11I 1705 ACATGT A^CATGT BspMI 443 GCAGGT ACCTGC(4/8) BsrBI 1017 GAGCGG CCGCTC(-3/-3) BstBI 1003 TTCGAA TT^CGAA BstEII 838 GGTGACC G^GT[ACGT]ACC BstPI 838 GGTGACC G^GT[ACGT]ACC Bsu36I 51 CCTCAGG CC^T[ACGT]AGG EaeI 532 TGGCCA [CT]^GGCC[GA] Eco81I 51 CCTCAGG CC^T[ACGT]AGG EcoNI 722 CCTACAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 838 GGTGACC G^GT[ACGT]ACC EcoT22I 393 ATGCAT ATGCA^T FauI 1019 GCGGG CCCGC(4/6) FspI 1225 TGCGCA TGC^GCA HapII 816 CCGG C^CGG HindIII 2061 AAGCTT A^AGCTT HpaI 1927 GTTAAC GTT^AAC HpaII 816 CCGG C^CGG MfeI 480 CAATTG C^AATTG MluNI 532 TGGCCA TGG^CCA MscI 532 TGGCCA TGG^CCA MslI 1812 CATAGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 816 CCGG C^CGG MunI 480 CAATTG C^AATTG NciI 816 CCGGG CC^[GC]GG NcoI 919 CCATGG C^CATGG NsiI 393 ATGCAT ATGCA^T NspI 1705 ACATGT [GA]CATG^[CT] NspV 1003 TTCGAA TT^CGAA PflMI 479 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 653 CACGTG CAC^GTG PmlI 653 CACGTG CAC^GTG PshBI 1680 ATTAAT AT^TAAT RcaI 1267 TCATGA T^CATGA SacI 185 GAGCTC GAGCT^C SapI 1574 GCTCTTC GCTCTTC(1/4) ScaI 1911 AGTACT AGT^ACT SfuI 1003 TTCGAA TT^CGAA SmlI 1066 CTCAAG C^T[CT][GA]AG Van91I 479 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1430 CCAGTGCCCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflIII 1705 ACATGT A^C[GA][CT]GT 1923 ACACGT Asp700I 1571 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1985 GAAAAGTTTC BanII 185 GAGCTC G[GA]GC[CT]^C 862 GAGCCC BclI 801 TGATCA T^GATCA 1294 TGATCA BfaI 1802 CTAG C^TAG 1995 CTAG BlpI 5 GCTGAGC GC^T[ACGT]AGC 1585 GCTAAGC BpmI 690 CTGGAG CTGGAG(16/14) 845 CTGGAG Bpu1102I 5 GCTGAGC GC^T[ACGT]AGC 1585 GCTAAGC BsrDI 429 CATTGC GCAATG(2/0) 1229 CATTGC CelII 5 GCTGAGC GC^T[ACGT]AGC 1585 GCTAAGC CfoI 433 GCGC GCG^C 1226 GCGC DraII 583 AGGGCCT [GA]G^G[ACGT]CC[CT] 1070 AGGCCCT EcoO109I 583 AGGGCCT [GA]G^G[ACGT]CC[CT] 1070 AGGCCCT FbaI 801 TGATCA T^GATCA 1294 TGATCA HhaI 433 GCGC GCG^C 1226 GCGC HinP1I 433 GCGC G^CGC 1226 GCGC HincII 221 GTTGAC GT[CT]^[GA]AC 1927 GTTAAC HindII 221 GTTGAC GT[CT]^[GA]AC 1927 GTTAAC MaeI 1802 CTAG C^TAG 1995 CTAG PstI 1383 CTGCAG CTGCA^G 1932 CTGCAG SspI 701 AATATT AAT^ATT 2036 AATATT TaqI 330 TCGA T^CGA 1004 TCGA TthHB8I 330 TCGA T^CGA 1004 TCGA XmnI 1571 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1985 GAAAAGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BmyI BsaJI BseRI BsgI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DrdI DsaI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII