##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BanI BbeI BbsI BcgI
BciVI BfrI BglII BlnI BpuAI
BsaBI BseAI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstYI BstZ17I
ClaI CpoI DraI DrdI Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HpaI KasI KpnI
MamI MflI MluI MroI NaeI
NarI NcoI NdeI NgoMIV NheI
NruI NsiI NspV PacI PaeR7I
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI XbaI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 620 GTCGAC GT^[AC][GT]AC
AcsI 1638 GAATTT [GA]^AATT[CT]
AgeI 109 ACCGGT A^CCGGT
AlwI 295 GATCC GGATC(4/5)
AocI 1735 CCTGAGG CC^T[ACGT]AGG
ApoI 1638 GAATTT [GA]^AATT[CT]
BbrPI 1297 CACGTG CAC^GTG
BsaAI 1297 CACGTG [CT]AC^GT[GA]
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsiWI 763 CGTACG C^GTACG
BsrBI 532 GAGCGG CCGCTC(-3/-3)
BssSI 1304 CTCGTG CACGAG(-5/-1)
BstAPI 185 GCAGCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1735 CCTGAGG CC^T[ACGT]AGG
DraIII 1262 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 5 CGGCCG C^GGCCG
EarI 1467 CTCTTC CTCTTC(1/4)
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
Eco81I 1735 CCTGAGG CC^T[ACGT]AGG
HindIII 824 AAGCTT A^AGCTT
Ksp632I 1467 CTCTTC CTCTTC(1/4)
KspI 517 CCGCGG CCGC^GG
MfeI 491 CAATTG C^AATTG
MseI 1654 TTAA T^TAA
MunI 491 CAATTG C^AATTG
NotI 4 GCGGCCGC GC^GGCCGC
NspI 147 GCATGC [GA]CATG^[CT]
PinAI 109 ACCGGT A^CCGGT
PmaCI 1297 CACGTG CAC^GTG
PmlI 1297 CACGTG CAC^GTG
SacII 517 CCGCGG CCGC^GG
SalI 620 GTCGAC G^TCGAC
SapI 1466 GCTCTTC GCTCTTC(1/4)
SexAI 1125 ACCAGGT A^CC[AT]GGT
SphI 147 GCATGC GCATG^C
Tru9I 1654 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 21 GACGTC GACGT^C
719 GACGTC
AspHI 809 GAGCAC G[AT]GC[AT]^C
1406 GTGCTC
BalI 141 TGGCCA TGG^CCA
483 TGGCCA
BbvCI 1717 GCTGAGG CCTCAGC(-5/-2)
1852 GCTGAGG
BclI 156 TGATCA T^GATCA
913 TGATCA
BlpI 287 GCTCAGC GC^T[ACGT]AGC
958 GCTCAGC
Bpu1102I 287 GCTCAGC GC^T[ACGT]AGC
958 GCTCAGC
BsaI 50 GAGACC GGTCTC(1/5)
1752 GAGACC
BsaWI 25 TCCGGT [AT]^CCGG[AT]
109 ACCGGT
BsiHKAI 809 GAGCAC G[AT]GC[AT]^C
1406 GTGCTC
BsmBI 1388 CGTCTC CGTCTC(1/5)
1782 CGTCTC
BsmI 1095 GCATTC GAATGC(1/-1)
1209 GCATTC
BsrDI 1051 CATTGC GCAATG(2/0)
1913 CATTGC
BsrFI 109 ACCGGT [GA]^CCGG[CT]
563 ACCGGC
CelII 287 GCTCAGC GC^T[ACGT]AGC
958 GCTCAGC
Cfr10I 109 ACCGGT [GA]^CCGG[CT]
563 ACCGGC
DsaI 393 CCACGG C^C[GA][CT]GG
517 CCGCGG
FbaI 156 TGATCA T^GATCA
913 TGATCA
MluNI 141 TGGCCA TGG^CCA
483 TGGCCA
MscI 141 TGGCCA TGG^CCA
483 TGGCCA
PflMI 451 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1261 CCACATGGTGG
SmaI 1026 CCCGGG CCC^GGG
1424 CCCGGG
TfiI 1326 GAATC G^A[AT]TC
1864 GAATC
Tsp509I 492 AATT ^AATT
1639 AATT
Van91I 451 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1261 CCACATGGTGG
XmaCI 1026 CCCGGG C^CCGGG
1424 CCCGGG
XmaI 1026 CCCGGG C^CCGGG
1424 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwNI ApaI AspEI AspI
AvaI AvaII BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI Eam1105I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflFI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TseI Tsp45I TspRI
Tth111I TthHB8I XcmI