################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BanI BbeI BbsI BcgI BciVI BfrI BglII BlnI BpuAI BsaBI BseAI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I ClaI CpoI DraI DrdI Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HpaI KasI KpnI MamI MflI MluI MroI NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 620 GTCGAC GT^[AC][GT]AC AcsI 1638 GAATTT [GA]^AATT[CT] AgeI 109 ACCGGT A^CCGGT AlwI 295 GATCC GGATC(4/5) AocI 1735 CCTGAGG CC^T[ACGT]AGG ApoI 1638 GAATTT [GA]^AATT[CT] BbrPI 1297 CACGTG CAC^GTG BsaAI 1297 CACGTG [CT]AC^GT[GA] BsiEI 5 CGGCCG CG[GA][CT]^CG BsiWI 763 CGTACG C^GTACG BsrBI 532 GAGCGG CCGCTC(-3/-3) BssSI 1304 CTCGTG CACGAG(-5/-1) BstAPI 185 GCAGCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1735 CCTGAGG CC^T[ACGT]AGG DraIII 1262 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 5 CGGCCG C^GGCCG EarI 1467 CTCTTC CTCTTC(1/4) EclXI 5 CGGCCG C^GGCCG Eco52I 5 CGGCCG C^GGCCG Eco81I 1735 CCTGAGG CC^T[ACGT]AGG HindIII 824 AAGCTT A^AGCTT Ksp632I 1467 CTCTTC CTCTTC(1/4) KspI 517 CCGCGG CCGC^GG MfeI 491 CAATTG C^AATTG MseI 1654 TTAA T^TAA MunI 491 CAATTG C^AATTG NotI 4 GCGGCCGC GC^GGCCGC NspI 147 GCATGC [GA]CATG^[CT] PinAI 109 ACCGGT A^CCGGT PmaCI 1297 CACGTG CAC^GTG PmlI 1297 CACGTG CAC^GTG SacII 517 CCGCGG CCGC^GG SalI 620 GTCGAC G^TCGAC SapI 1466 GCTCTTC GCTCTTC(1/4) SexAI 1125 ACCAGGT A^CC[AT]GGT SphI 147 GCATGC GCATG^C Tru9I 1654 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AatII 21 GACGTC GACGT^C 719 GACGTC AspHI 809 GAGCAC G[AT]GC[AT]^C 1406 GTGCTC BalI 141 TGGCCA TGG^CCA 483 TGGCCA BbvCI 1717 GCTGAGG CCTCAGC(-5/-2) 1852 GCTGAGG BclI 156 TGATCA T^GATCA 913 TGATCA BlpI 287 GCTCAGC GC^T[ACGT]AGC 958 GCTCAGC Bpu1102I 287 GCTCAGC GC^T[ACGT]AGC 958 GCTCAGC BsaI 50 GAGACC GGTCTC(1/5) 1752 GAGACC BsaWI 25 TCCGGT [AT]^CCGG[AT] 109 ACCGGT BsiHKAI 809 GAGCAC G[AT]GC[AT]^C 1406 GTGCTC BsmBI 1388 CGTCTC CGTCTC(1/5) 1782 CGTCTC BsmI 1095 GCATTC GAATGC(1/-1) 1209 GCATTC BsrDI 1051 CATTGC GCAATG(2/0) 1913 CATTGC BsrFI 109 ACCGGT [GA]^CCGG[CT] 563 ACCGGC CelII 287 GCTCAGC GC^T[ACGT]AGC 958 GCTCAGC Cfr10I 109 ACCGGT [GA]^CCGG[CT] 563 ACCGGC DsaI 393 CCACGG C^C[GA][CT]GG 517 CCGCGG FbaI 156 TGATCA T^GATCA 913 TGATCA MluNI 141 TGGCCA TGG^CCA 483 TGGCCA MscI 141 TGGCCA TGG^CCA 483 TGGCCA PflMI 451 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1261 CCACATGGTGG SmaI 1026 CCCGGG CCC^GGG 1424 CCCGGG TfiI 1326 GAATC G^A[AT]TC 1864 GAATC Tsp509I 492 AATT ^AATT 1639 AATT Van91I 451 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1261 CCACATGGTGG XmaCI 1026 CCCGGG C^CCGGG 1424 CCCGGG XmaI 1026 CCCGGG C^CCGGG 1424 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AcyI AfaI AhdI AluI AlwNI ApaI AspEI AspI AvaI AvaII BanII BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI Eam1105I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflFI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I TspRI Tth111I TthHB8I XcmI