##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspI AvaI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BciVI BfrI BglI BlnI BpuAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI EarI Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI Ksp632I
MamI MfeI MluI MluNI MroI
MscI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuII RcaI RsrII SapI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 855 GTCGAC GT^[AC][GT]AC
AcsI 1081 AAATTC [GA]^AATT[CT]
AhdI 1258 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1158 GATCC GGATC(4/5)
ApaI 1228 GGGCCC GGGCC^C
ApoI 1081 AAATTC [GA]^AATT[CT]
Asp700I 869 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1258 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 969 GAGCTC G[AT]GC[AT]^C
BbeI 942 GGCGCC GGCGC^C
BcgI 217 GCAAGCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1189 TGATCA T^GATCA
BlpI 13 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 13 GCTGAGC GC^T[ACGT]AGC
BsaAI 1041 CACGTA [CT]AC^GT[GA]
BsaI 1256 GAGACC GGTCTC(1/5)
BseRI 1000 CTCCTC GAGGAG(10/8)
BsgI 633 GTGCAG GTGCAG(16/14)
BsiHKAI 969 GAGCTC G[AT]GC[AT]^C
BsmFI 1217 GTCCC GGGAC(10/14)
Bsp1407I 841 TGTACA T^GTACA
BspMI 200 GCAGGT ACCTGC(4/8)
BsrBI 180 GAGCGG CCGCTC(-3/-3)
BsrDI 1152 CATTGC GCAATG(2/0)
BsrGI 841 TGTACA T^GTACA
BstAPI 284 GCAGGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 13 GCTGAGC GC^T[ACGT]AGC
DraI 1308 TTTAAA TTT^AAA
DrdI 258 GACTGCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1258 GACCTAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1189 TGATCA T^GATCA
HaeII 942 GGCGCC [GA]GCGC^[CT]
HincII 855 GTCGAC GT[CT]^[GA]AC
HindII 855 GTCGAC GT[CT]^[GA]AC
KasI 942 GGCGCC G^GCGCC
KspI 993 CCGCGG CCGC^GG
MaeIII 750 GTGAC ^GT[ACGT]AC
MseI 1309 TTAA T^TAA
NarI 942 GGCGCC GG^CGCC
NcoI 1232 CCATGG C^CATGG
NspI 691 GCATGC [GA]CATG^[CT]
PleI 1264 GAGTC GAGTC(4/5)
PstI 198 CTGCAG CTGCA^G
PvuI 175 CGATCG CGAT^CG
SacI 969 GAGCTC GAGCT^C
SacII 993 CCGCGG CCGC^GG
SalI 855 GTCGAC G^TCGAC
ScaI 1250 AGTACT AGT^ACT
SfoI 942 GGCGCC GGC^GCC
SphI 691 GCATGC GCATG^C
SspBI 841 TGTACA T^GTACA
Tru9I 1309 TTAA T^TAA
Tsp45I 750 GTGAC ^GT[GC]AC
Tsp509I 1082 AATT ^AATT
TspRI 623 CAGTG CA[GC]TG[ACGT][ACGT]^
XbaI 1137 TCTAGA T^CTAGA
XmnI 869 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BanI 849 GGCACC G^G[CT][GA]CC
942 GGCGCC
BanII 969 GAGCTC G[GA]GC[CT]^C
1228 GGGCCC
BfaI 1138 CTAG C^TAG
1261 CTAG
BglII 382 AGATCT A^GATCT
871 AGATCT
BsrFI 459 GCCGGC [GA]^CCGG[CT]
762 GCCGGC
BsrI 1086 CCAGT ACTGG(1/-1)
1248 CCAGT
BstYI 382 AGATCT [GA]^GATC[CT]
871 AGATCT
Cfr10I 459 GCCGGC [GA]^CCGG[CT]
762 GCCGGC
DraII 604 GGGGCCT [GA]G^G[ACGT]CC[CT]
1227 GGGGCCC
DraIII 717 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
1193 CACGGGGTG
EcoNI 298 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
608 CCTGTACCAGG
EcoO109I 604 GGGGCCT [GA]G^G[ACGT]CC[CT]
1227 GGGGCCC
HgaI 24 GACGC GACGC(5/10)
1215 GCGTC
MaeI 1138 CTAG C^TAG
1261 CTAG
MflI 382 AGATCT [GA]^GATC[CT]
871 AGATCT
MslI 719 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
803 CATGATGATG
NaeI 459 GCCGGC GCC^GGC
762 GCCGGC
NgoMIV 459 GCCGGC G^CCGGC
762 GCCGGC
SfcI 198 CTGCAG C^T[GA][CT]AG
1116 CTGTAG
SmlI 343 CTCAAG C^T[CT][GA]AG
972 CTCAAG
StuI 557 AGGCCT AGG^CCT
895 AGGCCT
XhoII 382 AGATCT [GA]^GATC[CT]
871 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaII BbvI BcnI BmyI BpmI
BsaHI BsaJI BsiEI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EagI EclXI
Eco52I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TfiI TseI TthHB8I XcmI