##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BlnI BsaAI
BsaBI BsaI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspMI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FbaI FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI NarI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 2001 GTAGAC GT^[AC][GT]AC
AcyI 1019 GACGCC G[GA]^CG[CT]C
AflIII 369 ACATGT A^C[GA][CT]GT
ApaI 60 GGGCCC GGGCC^C
Asp700I 244 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1784 CTCGGG C^[CT]CG[GA]G
BcnI 63 CCCGG CC^[GC]GG
BglII 2051 AGATCT A^GATCT
BlpI 1900 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1900 GCTCAGC GC^T[ACGT]AGC
BsaHI 1019 GACGCC G[GA]^CG[CT]C
BsaWI 706 TCCGGT [AT]^CCGG[AT]
BsgI 755 CTGCAC GTGCAG(16/14)
BsoBI 1784 CTCGGG C^[CT]CG[GA]G
BspLU11I 369 ACATGT A^CATGT
BsrBI 78 CCGCTC CCGCTC(-3/-3)
BsrDI 1802 GCAATG GCAATG(2/0)
BstAPI 292 GCAATTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1900 GCTCAGC GC^T[ACGT]AGC
EcoNI 1666 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 895 GAATTC G^AATTC
FauI 77 CCCGC CCCGC(4/6)
Hin1I 1019 GACGCC G[GA]^CG[CT]C
MfeI 293 CAATTG C^AATTG
MspA1I 1259 CAGCTG C[AC]G^C[GT]G
MunI 293 CAATTG C^AATTG
NciI 63 CCCGG CC^[GC]GG
PleI 451 GAGTC GAGTC(4/5)
PstI 88 CTGCAG CTGCA^G
PvuII 1259 CAGCTG CAG^CTG
SacI 326 GAGCTC GAGCT^C
SfaNI 298 GATGC GCATC(5/9)
SmlI 493 CTTGAG C^T[CT][GA]AG
SphI 1424 GCATGC GCATG^C
XbaI 2048 TCTAGA T^CTAGA
XmnI 244 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
BamHI 696 GGATCC G^GATCC
853 GGATCC
BanI 645 GGTGCC G^G[CT][GA]CC
1701 GGCACC
BanII 60 GGGCCC G[GA]GC[CT]^C
326 GAGCTC
BbsI 408 GAAGAC GAAGAC(2/6)
1246 GAAGAC
BciVI 159 GGATAC GTATCC(6/5)
1977 GGATAC
BpmI 1377 CTCCAG CTGGAG(16/14)
1484 CTCCAG
BpuAI 408 GAAGAC GAAGAC(2/6)
1246 GAAGAC
BsmFI 462 GGGAC GGGAC(10/14)
1710 GGGAC
BsrFI 70 GCCGGC [GA]^CCGG[CT]
1022 GCCGGC
Cfr10I 70 GCCGGC [GA]^CCGG[CT]
1022 GCCGGC
DraII 462 GGGACCT [GA]G^G[ACGT]CC[CT]
1743 GGGTCCT
DsaI 1767 CCGTGG C^C[GA][CT]GG
1776 CCGTGG
EarI 142 GAAGAG CTCTTC(1/4)
1604 GAAGAG
EcoO109I 462 GGGACCT [GA]G^G[ACGT]CC[CT]
1743 GGGTCCT
EcoT22I 725 ATGCAT ATGCA^T
1229 ATGCAT
HaeII 38 AGCGCC [GA]GCGC^[CT]
1355 AGCGCC
HgaI 1019 GACGC GACGC(5/10)
1854 GCGTC
Ksp632I 142 GAAGAG CTCTTC(1/4)
1604 GAAGAG
MaeII 684 ACGT A^CGT
926 ACGT
MslI 389 CATTTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
822 CATTTCTGTG
NaeI 70 GCCGGC GCC^GGC
1022 GCCGGC
NgoMIV 70 GCCGGC G^CCGGC
1022 GCCGGC
NsiI 725 ATGCAT ATGCA^T
1229 ATGCAT
NspI 369 ACATGT [GA]CATG^[CT]
1424 GCATGC
PpuMI 462 GGGACCT [GA]G^G[AT]CC[CT]
1743 GGGTCCT
SspI 246 AATATT AAT^ATT
1167 AATATT
TaqI 275 TCGA T^CGA
1202 TCGA
TthHB8I 275 TCGA T^CGA
1202 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BbvI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI Bsp1286I BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfcI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII