##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccIII AclI AcyI AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AvrII BbeI BbrPI
BcgI BclI BglII BlnI BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI Hin1I HincII HindII
HpaI KasI MamI MfeI MluI
MroI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
Acc65I 1312 GGTACC G^GTACC
AcsI 1001 AAATTC [GA]^AATT[CT]
AflII 1031 CTTAAG C^TTAAG
ApaI 1490 GGGCCC GGGCC^C
ApoI 1001 AAATTC [GA]^AATT[CT]
Asp718I 1312 GGTACC G^GTACC
AspHI 279 GAGCAC G[AT]GC[AT]^C
AspI 501 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 662 GGACC G^G[AT]CC
AviII 1269 TGCGCA TGC^GCA
BalI 1281 TGGCCA TGG^CCA
BamHI 906 GGATCC G^GATCC
BanI 1312 GGTACC G^G[CT][GA]CC
BbsI 1579 GTCTTC GAAGAC(2/6)
BbvCI 26 GCTGAGG CCTCAGC(-5/-2)
BfaI 1027 CTAG C^TAG
BfrI 1031 CTTAAG C^TTAAG
BglI 327 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1504 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1504 GCTGAGC GC^T[ACGT]AGC
BpuAI 1579 GTCTTC GAAGAC(2/6)
BsaWI 1264 TCCGGT [AT]^CCGG[AT]
BsgI 1345 GTGCAG GTGCAG(16/14)
BsiHKAI 279 GAGCAC G[AT]GC[AT]^C
BsmBI 1604 CGTCTC CGTCTC(1/5)
BsmI 529 GCATTC GAATGC(1/-1)
BsrBI 943 GAGCGG CCGCTC(-3/-3)
BsrDI 1584 CATTGC GCAATG(2/0)
BsrFI 701 GCCGGT [GA]^CCGG[CT]
BstXI 1538 CCAAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1504 GCTGAGC GC^T[ACGT]AGC
Cfr10I 701 GCCGGT [GA]^CCGG[CT]
DraIII 1116 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 990 CTCTTC CTCTTC(1/4)
FspI 1269 TGCGCA TGC^GCA
HgaI 1429 GCGTC GACGC(5/10)
KpnI 1312 GGTACC GGTAC^C
Ksp632I 990 CTCTTC CTCTTC(1/4)
KspI 2 CCGCGG CCGC^GG
MaeI 1027 CTAG C^TAG
MluNI 1281 TGGCCA TGG^CCA
MscI 1281 TGGCCA TGG^CCA
PflFI 501 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1115 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 661 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 330 CAGCTG CAG^CTG
SacII 2 CCGCGG CCGC^GG
SmaI 1292 CCCGGG CCC^GGG
SmlI 1031 CTTAAG C^T[CT][GA]AG
StuI 965 AGGCCT AGG^CCT
TaqI 832 TCGA T^CGA
Tth111I 501 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 832 TCGA T^CGA
Van91I 1115 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 232 CCATCAAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1292 CCCGGG C^CCGGG
XmaI 1292 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1136 GTAGAC GT^[AC][GT]AC
1403 GTAGAC
AccII 3 CGCG CG^CG
33 CGCG
AvaI 71 CTCGGG C^[CT]CG[GA]G
1292 CCCGGG
BanII 325 GAGCCC G[GA]GC[CT]^C
1490 GGGCCC
BciVI 468 GGATAC GTATCC(6/5)
1261 GTATCC
BpmI 582 CTCCAG CTGGAG(16/14)
633 CTGGAG
BsmFI 568 GGGAC GGGAC(10/14)
661 GGGAC
BsoBI 71 CTCGGG C^[CT]CG[GA]G
1292 CCCGGG
BspHI 922 TCATGA T^CATGA
1717 TCATGA
BstUI 3 CGCG CG^CG
33 CGCG
BstYI 610 AGATCC [GA]^GATC[CT]
906 GGATCC
HaeII 838 AGCGCC [GA]GCGC^[CT]
1286 AGCGCC
HindIII 318 AAGCTT A^AGCTT
360 AAGCTT
MflI 610 AGATCC [GA]^GATC[CT]
906 GGATCC
MslI 284 CATGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1369 CACGTTGATG
MvnI 3 CGCG CG^CG
33 CGCG
PstI 44 CTGCAG CTGCA^G
600 CTGCAG
RcaI 922 TCATGA T^CATGA
1717 TCATGA
Tsp509I 1002 AATT ^AATT
1445 AATT
XhoII 610 AGATCC [GA]^GATC[CT]
906 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I TspRI