##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspI AvrII
BamHI BbrPI BcgI BciVI BclI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsiWI BsmBI Bsp1407I BspDI
BspLU11I BsrBI BsrGI BssHII BstAPI
BstBI Bsu36I CelII ClaI CpoI
DrdI Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KpnI KspI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AccI 1762 GTATAC GT^[AC][GT]AC
AccIII 626 TCCGGA T^CCGGA
AcsI 1671 AAATTT [GA]^AATT[CT]
AcyI 329 GGCGCC G[GA]^CG[CT]C
AflII 205 CTTAAG C^TTAAG
AhdI 607 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1386 GGGCCC GGGCC^C
ApoI 1671 AAATTT [GA]^AATT[CT]
Asp700I 102 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 607 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 149 TGCGCA TGC^GCA
BbeI 329 GGCGCC GGCGC^C
BbsI 183 GAAGAC GAAGAC(2/6)
BbvCI 1580 CCTCAGC CCTCAGC(-5/-2)
BfrI 205 CTTAAG C^TTAAG
BpuAI 183 GAAGAC GAAGAC(2/6)
BsaHI 329 GGCGCC G[GA]^CG[CT]C
BsaI 1100 GAGACC GGTCTC(1/5)
BsaWI 626 TCCGGA [AT]^CCGG[AT]
BseAI 626 TCCGGA T^CCGGA
BsgI 529 CTGCAC GTGCAG(16/14)
BsmAI 1100 GAGAC GTCTC(1/5)
BspEI 626 TCCGGA T^CCGGA
BspHI 416 TCATGA T^CATGA
BspMI 568 ACCTGC ACCTGC(4/8)
BsrDI 377 GCAATG GCAATG(2/0)
BsrFI 973 GCCGGC [GA]^CCGG[CT]
Bst1107I 1762 GTATAC GTA^TAC
BstZ17I 1762 GTATAC GTA^TAC
Cfr10I 973 GCCGGC [GA]^CCGG[CT]
DraI 1051 TTTAAA TTT^AAA
EagI 503 CGGCCG C^GGCCG
Eam1105I 607 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 118 CTCTTC CTCTTC(1/4)
EclXI 503 CGGCCG C^GGCCG
Eco52I 503 CGGCCG C^GGCCG
EcoNI 117 CCTCTTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 149 TGCGCA TGC^GCA
HaeII 329 GGCGCC [GA]GCGC^[CT]
Hin1I 329 GGCGCC G[GA]^CG[CT]C
HindIII 202 AAGCTT A^AGCTT
KasI 329 GGCGCC G^GCGCC
Ksp632I 118 CTCTTC CTCTTC(1/4)
MroI 626 TCCGGA T^CCGGA
NaeI 973 GCCGGC GCC^GGC
NarI 329 GGCGCC GG^CGCC
NcoI 933 CCATGG C^CATGG
NgoMIV 973 GCCGGC G^CCGGC
PleI 954 GAGTC GAGTC(4/5)
PpuMI 479 AGGACCT [GA]G^G[AT]CC[CT]
PstI 176 CTGCAG CTGCA^G
PvuII 1154 CAGCTG CAG^CTG
RcaI 416 TCATGA T^CATGA
SfcI 176 CTGCAG C^T[GA][CT]AG
SfoI 329 GGCGCC GGC^GCC
SmlI 205 CTTAAG C^T[CT][GA]AG
SspI 861 AATATT AAT^ATT
StuI 354 AGGCCT AGG^CCT
TfiI 1065 GAATC G^A[AT]TC
XmnI 102 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 644 CGCG CG^CG
1496 CGCG
AvaI 1165 CTCGGG C^[CT]CG[GA]G
1538 CTCGGG
BanI 329 GGCGCC G^G[CT][GA]CC
938 GGCACC
BcnI 444 CCGGG CC^[GC]GG
1506 CCGGG
BfaI 1110 CTAG C^TAG
1523 CTAG
BglI 321 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1505 GCCGGGCAGGC
BpmI 948 CTCCAG CTGGAG(16/14)
1660 CTGGAG
BseRI 664 CTCCTC GAGGAG(10/8)
409 GAGGAG
BsiEI 503 CGGCCG CG[GA][CT]^CG
1403 CGGTCG
BsmI 498 GAATGC GAATGC(1/-1)
1262 GCATTC
BsoBI 1165 CTCGGG C^[CT]CG[GA]G
1538 CTCGGG
BssSI 707 CTCGTG CACGAG(-5/-1)
1443 CTCGTG
BstEII 744 GGTCACC G^GT[ACGT]ACC
1253 GGTCACC
BstPI 744 GGTCACC G^GT[ACGT]ACC
1253 GGTCACC
BstUI 644 CGCG CG^CG
1496 CGCG
BstYI 314 AGATCC [GA]^GATC[CT]
1227 AGATCC
DraIII 285 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
747 CACCGTGTG
EcoO65I 744 GGTCACC G^GT[ACGT]ACC
1253 GGTCACC
FauI 1105 CCCGC CCCGC(4/6)
1232 CCCGC
HgaI 782 GACGC GACGC(5/10)
1380 GACGC
HinfI 954 GAGTC G^A[ACGT]TC
1065 GAATC
MaeI 1110 CTAG C^TAG
1523 CTAG
MflI 314 AGATCC [GA]^GATC[CT]
1227 AGATCC
MspA1I 579 CAGCGG C[AC]G^C[GT]G
1154 CAGCTG
MvnI 644 CGCG CG^CG
1496 CGCG
NciI 444 CCGGG CC^[GC]GG
1506 CCGGG
PflMI 9 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
28 CCAACGCCTGG
SacI 412 GAGCTC GAGCT^C
439 GAGCTC
Van91I 9 CCATCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
28 CCAACGCCTGG
XhoII 314 AGATCC [GA]^GATC[CT]
1227 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanII BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I